细胞生物学

分类

    现刊
    Pea Aphid Rearing, Bacterial Infection and Hemocyte Phagocytosis Assay
    豌豆蚜虫饲养、细菌感染及血细胞吞噬功能测定
    作者:Li Ma, Lu Liu and Zhiqiang Lu日期:12/20/2020,浏览量:1198,Q&A: 0
    [Abstract]

    Insects rely on the simple but effective innate immune system to combat infection. Cellular and humoral responses are interconnected and synergistic in insects’ innate immune system. Phagocytosis is one major cellular response. It is difficult to collect clean hemolymph from the small insect like pea aphid. Here, we provide a practicable

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    Differentiation of Mouse Embryonic Stem Cells to Neuronal Cells Using Hanging Droplets and Retinoic Acid
    利用悬滴和维甲酸诱导小鼠胚胎干细胞向神经元细胞的分化
    作者:Jeroen Witteveldt and Sara Macias日期:11/05/2019,浏览量:3412,Q&A: 0
    [Abstract] Controlled differentiation of embryonic stem cells is an essential tool in stem cell research. In this protocol, we describe a simple differentiation protocol involving the induction of embryoid body formation in mouse embryonic stem cells (mESC) using hanging droplets, followed by differentiation into a neuronal lineage.
    Optogenetic Inactivation of Transcription Factors in the Early Embryo of Drosophila
    果蝇早期胚胎中的光遗传失活转录因子
    作者:Stephen L. McDaniel and Melissa M. Harrison日期:07/05/2019,浏览量:2735,Q&A: 0
    [Abstract] The early embryo of Drosophila melanogaster exists as a rapidly dividing syncytium of nuclei that are transcriptionally silent. Maternally deposited factors are required to awaken the genome and assist in the transition from maternal to zygotic control of development. Because many of these essential factors are maternally deposited and ...
    Measurement of Respiration Rate in Live Caenorhabditis elegans
    秀丽隐杆线虫活体呼吸速率的测定
    作者:Li Fang Ng and Jan Gruber日期:05/20/2019,浏览量:5391,Q&A: 0
    [Abstract] Mitochondrial function and dysfunction are at the core of aging and involved in many age-dependent diseases. Rate of oxygen consumption is a measure of mitochondrial function and energy production rate. The nematode Caenorhabditis elegans (C. elegans) offers an opportunity to study “living” mitochondria without the need for ...
    Eimeria vermiformis Infection Model of Murine Small Intestine
    蠕形艾美耳球虫对小鼠小肠的感染模型
    作者:Patrícia Figueiredo-Campos, Cristina Ferreira, Birte Blankenhaus and Marc Veldhoen日期:12/20/2018,浏览量:3506,Q&A: 0
    [Abstract] Eimeria vermiformis is a tissue specific, intracellular protozoan that infects the murine small intestinal epithelia, which has been widely used as a coccidian model to study mucosal immunology. This mouse infection model is valuable to investigate the mechanisms of host protection against primary and secondary infection in the small ...
    Maintenance of Schmidtea mediterranea in the Laboratory
    实验室真涡虫的保存
    作者:Nídia de Sousa and Teresa Adell日期:10/05/2018,浏览量:4592,Q&A: 0
    [Abstract] In the last years, planarians have emerged as a unique model animal for studying regeneration and stem cells biology. Although their remarkable regenerative abilities are known for a long time, only recently the molecular tools to understand the biology of planarian stem cells and the fundamentals of their regenerative process have been ...