微生物学

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    现刊
    COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR
    COVID-19样本汇集:从RNA提取到实时定量RT-PCR
    [Abstract]

    The COVID-19 pandemic requires mass screening to identify those infected for isolation and quarantine. Individually screening large populations for the novel pathogen, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is costly and requires a lot of resources. Sample pooling methods improve the efficiency of mass screening and consume

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    A Sensitive and Specific PCR-based Assay to Quantify Hepatitis B Virus Covalently Closed Circular (ccc) DNA while Preserving Cellular DNA
    一种在保留细胞DNA的同时定量检测乙型肝炎病毒共价闭合环状(ccc)DNA的灵敏、特异PCR方法
    作者:Benno Zehnder, Stephan Urban and Thomas Tu日期:04/20/2021,浏览量:2637,Q&A: 0
    [Abstract]

    Hepatitis B virus (HBV) is the major cause of liver diseases and liver cancer worldwide. After infecting hepatocytes, the virus establishes a stable episome (covalently closed circular DNA, or cccDNA) that serves as the template for all viral transcripts. Specific and accurate quantification of cccDNA is difficult because infected cells contain

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    Detection and Quantification of African Swine Fever Virus in MA-104 Cells
    非洲猪瘟病毒在MA-104细胞中的检测与定量
    [Abstract]

    Detection of live African swine fever virus (ASFV) has historically relied on the use of primary swine macrophages (PSM). PSM do not replicate and have to be isolated fresh from donor swine. We previously identified that a MA-104 cells (ATCC #CRL-2378.1), a commercially available cell line isolated from African green monkey (Cercopithecus

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    Colorimetric RT-LAMP and LAMP-sequencing for Detecting SARS-CoV-2 RNA in Clinical Samples
    比色RT-LAMP和LAMP测序检测临床样本中SARS-CoV-2 RNA
    作者:Konrad Herbst, Matthias Meurer, Daniel Kirrmaier, Simon Anders, Michael Knop and Viet Loan Dao Thi日期:03/20/2021,浏览量:2670,Q&A: 0
    [Abstract]

    During pandemics, such as the one caused by SARS-CoV-2 coronavirus, simple methods to rapidly test large numbers of people are needed. As a faster and less resource-demanding alternative to detect viral RNA by conventional qPCR, we used reverse transcription loop-mediated isothermal amplification (RT-LAMP). We previously established colorimetric

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    A One-enzyme RT-qPCR Assay for SARS-CoV-2, and Procedures for Reagent Production
    SARS-CoV-2单酶RT-qPCR检测方法及试剂生产程序
    作者:Sanchita Bhadra, Andre C. Maranhao, Inyup Paik and Andrew D. Ellington日期:01/20/2021,浏览量:1100,Q&A: 1
    [Abstract]

    Given the scale of the ongoing COVID-19 pandemic, the need for reliable, scalable testing, and the likelihood of reagent shortages, especially in resource-poor settings, we have developed an RT-qPCR assay that relies on an alternative to conventional viral reverse transcriptases, a thermostable reverse transcriptase/DNA polymerase (RTX) (Ellefson

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    Evaluation of the Sequence Variability within the PCR Primer/Probe Target Regions of the SARS-CoV-2 Genome
    SARS-CoV-2基因组PCR引物/探针靶区序列变异性评价
    作者:Kashif Aziz Khan and Peter Cheung日期:12/20/2020,浏览量:1831,Q&A: 0
    [Abstract] Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; initially named 2019-nCoV) is responsible for the recent coronavirus disease (COVID-19) pandemic, and polymerase chain reaction (PCR) is the current standard method for diagnosis from patient samples. As PCR assays are prone to sequence mismatches due to mutations in the viral genome, it ...
    Colorimetric RT-LAMP Methods to Detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
    严重急性呼吸综合征冠状病毒2(SARS-CoV-2)的RT-LAMP比色法检测
    作者:Gun-Soo Park, Seung-Hwa Baek, Keunbon Ku, Seong Jun Kim, Seung Il Kim, Bum-Tae Kim and Jin-Soo Maeng日期:11/05/2020,浏览量:2174,Q&A: 0
    [Abstract] Standard diagnostic methods of Coronavirus Disease 2019 (COVID-19) rely on RT-qPCR technique which have limited point-of-care test (POCT) potential due to necessity of dedicated equipment and specialized personnel. LAMP, an isothermal nucleic acid amplification test (NAAT), is a promising technique that may substitute RT-qPCR for POCT of genomic ...
    A Protocol for Simple, Rapid, and Direct Detection of SARS-CoV-2 from clinical samples, using Reverse Transcribed Loop-Mediated Isothermal Amplification (RT-LAMP)
    使用逆转录环介导等温扩增(RT-LAMP)从临床样本中简单、快速、直接检测SARS-CoV-2
    [Abstract] SARS-CoV-2 has quickly spread all around the globe causing illness and wide damages. Most countries were unprepared for such a rapid spread and crisis. This led to various strategies for effective control of the new pandemic. A key aspect in all countries was to effectively test the population for the virus. Most countries chose a lockdown ...
    Screening Method for CRISPR/Cas9 Inhibition of a Human DNA Virus: Herpes Simplex Virus
    人单纯疱疹病毒CRISPR/Cas9抑制作用的筛选方法
    [Abstract] The efficiency of cleavage of individual CRISPR/Cas9-sgRNAs remains difficult to predict based on the CRISPR target sequence alone. Different intracellular environments (dependent on cell type or cell cycle state for example) may affect sgRNA efficiency by altering accessibility of genomic DNA through DNA modifications such as epigenetic marks and ...
    Paper Lateral Flow Biosensor for Nodavirus Reverse Transcribed RNA Detection
    用于诺达病毒逆转录RNA检测的纸侧向流生物传感器
    作者:Dimitra K. Toubanaki and Evdokia Karagouni日期:08/05/2020,浏览量:1427,Q&A: 0
    [Abstract] Paper nanobiosensors have been established as an excellent platform for analysis of veterinary and human pathogens causing various diseases. Especially, lateral flow assays or biosensors ideal for sensitive, rapid, robust and accurate analysis in laboratory setups and on-site analysis. Viral RNA detection is of great importance for public health ...