细胞生物学

分类

    现刊
    Plasmodium cynomolgi Berok Growth Inhibition Assay by Thiol-reactive Probe Based Flow Cytometric Measurement
    硫醇反应探针流式细胞术检测食蟹猴疟原虫生长抑制
    作者:Jessica Jie Ying Ong, Bruce Russell and Jin-Hee Han日期:09/05/2021,浏览量:646,Q&A: 0
    [Abstract]

    The relapsing malaria species, Plasmodium vivax, is the most widely distributed and difficult-to-treat cause of human malaria. The merozoites of P. vivax preferentially invade ephemeral human CD71+ reticulocytes (nascent reticulocytes), thereby limiting the development of a robust continuous culture in vitro. Fortunately, P. vivax’s sister

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    Isolation of Single Cells from Mouse Periodontal Ligament
    小鼠牙周膜单细胞的分离
    作者:Jing Zhao and Paul Sharpe日期:08/20/2021,浏览量:894,Q&A: 0
    [Abstract]

    The periodontal ligament (PDL) is an essential tissue connecting teeth and bone. It is a complex tissue specifically designed to absorb the forces of mastication; analysis of its multiple cell populations is important to understand its function and the cell changes associated with periodontal disease. Cells in the periodontal ligament are not

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    A Novel Method to Make Polyacrylamide Gels with Mechanical Properties Resembling those of Biological Tissues
    一种制备具有类似生物组织力学性能的聚丙烯酰胺凝胶的新方法  
    作者:Katarzyna Pogoda, Elisabeth E. Charrier and Paul A. Janmey日期:08/20/2021,浏览量:949,Q&A: 0
    [Abstract]

    Studies characterizing how cells respond to the mechanical properties of their environment have been enabled by the use of soft elastomers and hydrogels as substrates for cell culture. A limitation of most such substrates is that, although their elastic properties can be accurately controlled, their viscous properties cannot, and cells

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    One-step White Blood Cell Extracellular Staining Method for Flow Cytometry
    流式细胞术白细胞细胞外一步染色法
    作者:Ines Ait Belkacem, Pénélope Bourgoin, Jean Marc Busnel, Franck Galland and Fabrice Malergue日期:08/20/2021,浏览量:683,Q&A: 0
    [Abstract]

    Flow cytometry is a powerful analytical technique that is increasingly used in scientific investigations and healthcare; however, it requires time-consuming, multi-step sample procedures, which limits its use to specialized laboratories. In this study, we propose a new universal one-step method in which white blood cell staining and red blood cell

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    Cell-attached and Whole-cell Patch-clamp Recordings of Dopamine Neurons in the Substantia Nigra Pars Compacta of Mouse Brain Slices
    小鼠脑片黑质致密部多巴胺神经元的细胞贴附和全细胞膜片钳记录
    作者:Stefano Cattaneo, Maria Regoni, Jenny Sassone and Stefano Taverna日期:08/05/2021,浏览量:792,Q&A: 0
    [Abstract]

    The Substantia Nigra pars compacta (SNc) is a midbrain dopaminergic nucleus that plays a key role in modulating motor and cognitive functions. It is crucially involved in several disorders, particularly Parkinson’s disease, which is characterized by a progressive loss of SNc dopaminergic cells. Electrophysiological studies on SNc neurons are

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    Isolation of Microglia and Analysis of Protein Expression by Flow Cytometry: Avoiding the Pitfall of Microglia Background Autofluorescence
    小胶质细胞的分离及蛋白表达的流式细胞仪分析:避免小胶质细胞背景荧光的陷阱
    作者:Jeremy C. Burns, Richard M. Ransohoff and Michaël Mingueneau日期:07/20/2021,浏览量:1488,Q&A: 0
    [Abstract]

    Microglia are a unique type of tissue-resident innate immune cell found within the brain, spinal cord, and retina. In the healthy nervous system, their main functions are to defend the tissue against infectious microbes, support neuronal networks through synapse remodeling, and clear extracellular debris and dying cells through phagocytosis. Many

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    Electrophysiological Properties of Neurons: Current-Clamp Recordings in Mouse Brain Slices and Firing-Pattern Analysis
    神经元的电生理特性:小鼠脑切片中的电流钳记录和放电模式分析
    作者:Elina Nagaeva, Ivan Zubarev and Esa R. Korpi日期:06/20/2021,浏览量:1476,Q&A: 0
    [Abstract]

    Characterization of an electrically active cell, such as a neuron, demands measurement of its electrical properties. Due to differences in gene activation, location, innervation patterns, and functions, the millions of neurons in the mammalian brain are tremendously diverse in their membrane characteristics and abilities to generate action

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    Sex-specific Separation of Plasmodium falciparum Gametocyte Populations
    恶性疟原虫配子细胞群体的性别特异性分离
    作者:Melanie C. Ridgway, Daniela Cihalova and Alexander G. Maier日期:06/05/2021,浏览量:1275,Q&A: 0
    [Abstract]

    Plasmodium falciparum is a unicellular eukaryotic parasite that causes malaria in humans. The parasite is spread by Anopheles mosquitoes after ingestion of sexual stage parasites known as gametocytes. Malaria transmission depends on parasites switching from the disease-causing asexual blood forms to male and female gametocytes. The current

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    In vitro and In vivo CD8+ T Cell Suppression Assays
    CD8+ T细胞体外和体内抑制试验
    作者:Lu Xie, Guihuan Liu, Yanjun Liu and Yuming Yu日期:05/20/2021,浏览量:2405,Q&A: 0
    [Abstract]

    CD8+CD28− T suppressor cells (Ts) have been documented to promote immune tolerance by suppressing effector T cell responses to alloantigens following transplantation. The suppressive function of T cells has been defined as the inhibitory effect of Ts on the proliferation rate of effector T cells. 3H-thymidine is a classical immunological

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    FACS Enrichment of Total Interstitial Cells and Fibroblasts from Adult Mouse Ventricles
    流式细胞术富集成年小鼠心室间质细胞和成纤维细胞
    作者:Nona Farbehi, Vaibhao Janbandhu, Robert E. Nordon and Richard P. Harvey日期:05/20/2021,浏览量:1481,Q&A: 0
    [Abstract]

    Besides cardiomyocytes, the heart contains numerous interstitial cell types, including cardiac fibroblasts, endothelial cells, immune (myeloid and lymphoid) cells, and mural cells (pericytes and vascular smooth muscle cells), which play key roles in heart repair, regeneration, and disease. We recently published a comprehensive map of cardiac

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