微生物学

分类

    现刊
    Cell-free Translation: Preparation and Validation of Translation-competent Extracts from Saccharomyces cerevisiae
    无细胞翻译:酿酒酵母翻译活性提取物的制备和验证
    作者:Brandon M. Trainor, Anton A. Komar, Dimitri G. Pestov and Natalia Shcherbik日期:09/20/2021,浏览量:710,Q&A: 0
    [Abstract]

    Cell-free translation is a powerful technique for in vitro protein synthesis. While cell-free translation platforms prepared from bacterial, plant, and mammalian cells are commercially available, yeast-based translation systems remain proprietary knowledge of individual labs. Here, we provide a detailed protocol for simple, fast, and

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    Development and Quantitation of Pseudomonas aeruginosa Biofilms after in vitro Cultivation in Flow-reactors
    流动反应器体外培养铜绿假单胞菌生物膜的研制与定量
    作者:Yingdan Zhang, Jingru Zhao, Hang Cheng, Jing Wang, Liang Yang and Haihua Liang日期:08/20/2021,浏览量:445,Q&A: 0
    [Abstract]

    Characterization of biofilm formation and metabolic activities is critical to investigating biofilm interactions with environmental factors and illustrating biofilm regulatory mechanisms. An appropriate in vitro model that mimics biofilm in vivo habitats therefore demands accurate quantitation and investigation of biofilm-associated activities.

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    PCR-mediated One-day Synthesis of Guide RNA for the CRISPR/Cas9 System
    CRISPR/Cas9系统的PCR介导的一日合成向导RNA
    作者:Naim Hassan, Farhana Easmin, Keisuke Ekino and Satoshi Harashima日期:07/05/2021,浏览量:1655,Q&A: 0
    [Abstract]

    Nowadays, CRISPR (clustered regularly interspaced short palindromic repeats) and the CRISPR-associated protein (Cas9) system play a major role in genome editing. To target the desired sequence of the genome successfully, guide RNA (gRNA) is indispensable for the CRISPR/Cas9 system. To express gRNA, a plasmid expressing the gRNA sequence is

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    Transcriptional Run-on: Measuring Nascent Transcription at Specific Genomic Sites in Yeast
    连缀转录:测定酵母特定基因组位点的新生转录
    作者:Victoria Begley, Lola de Miguel-Jiménez and Sebastián Chávez日期:06/20/2021,浏览量:987,Q&A: 0
    [Abstract]

    DNA transcription by RNA polymerases has always interested the scientific community as it is one of the most important processes involved in genome expression. This has led scientists to come up with different protocols allowing analysis of this process in specific locations across the genome by quantitating the amount of RNA polymerases

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    Defined Mutant Library Sequencing (DML-Seq) for Identification of Conditional Essential Genes
    确定突变体文库测序(DML-Seq)用于鉴定的条件必需基因
    作者:Shuai Shao, Lifan Wei, Feng Xia, Yuanxing Zhang and Qiyao Wang日期:03/05/2021,浏览量:2046,Q&A: 0
    [Abstract]

    Transposon insertion sequencing (TIS) is an emerging technique which utilizes a massive transposon mutant library to screen specific phenotype and determine the conditional essential genetic requirements for bacterial fitness under distinct conditions combined with high-throughput parallel sequencing technology. Compared with a massive mutant

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    Bacterial Conjugation Protocol for Ruminant Mycoplasmas
    反刍动物支原体细菌接合的实验方法
    作者:Eveline Sagné, Christine Citti and Emilie Dordet-Frisoni日期:01/20/2021,浏览量:1057,Q&A: 0
    [Abstract]

    In Mycoplasma agalactiae, two simultaneous processes of DNA transfer have been described that require direct cell-to-cell contact and are similar to conjugation. One involves the self-transmission of an integrative conjugative element (ICE) while the second concerns the horizontal transfer of large and small fragments of chromosomal DNA. Here, we

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    Candida albicans Culture, Cell Harvesting, and Total RNA Extraction
    白念珠菌培养、细胞收获和总RNA提取
    作者:Max V. Cravener and Aaron P. Mitchell日期:11/05/2020,浏览量:1557,Q&A: 0
    [Abstract]

    Transcriptional analysis has become a cornerstone of biological research, and with the advent of cheaper and more efficient sequencing technology over the last decade, there exists a need for high-yield and efficient RNA extraction techniques. Fungi such as the human pathogen Candida albicans present a unique obstacle to RNA purification in the

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    Estimation of the Minimum Number of Replication Origins Per Chromosome in any Organism
    任何生物体内每个染色体的最小复制起源点数目的估计
    作者:Marcelo S. da Silva日期:10/20/2020,浏览量:1717,Q&A: 0
    [Abstract] Eukaryote nuclear genomes predominantly replicate through multiple replication origins. The number of replication origins activated per chromosome during the S-phase duration may vary according to many factors, but the predominant one is replication stress. Several studies have applied different approaches to estimate the number and map the ...
    TetR Regulated in vivo Repression Technology to Identify Conditional Gene Silencing in Genetically Engineerable Bacteria Using Vibrio cholerae Murine Infections as Model System
    以小鼠霍乱弧菌感染为模型系统的TetR调控体内抑制技术鉴定基因工程菌的条件基因沉默
    作者:Franz G. Zingl, Fabian Mitterer, Himadri B. Thapa and Stefan Schild日期:10/05/2020,浏览量:1174,Q&A: 0
    [Abstract] Investigation of bacterial gene regulation upon environmental changes is still a challenging task. For example, Vibrio cholerae, a pathogen of the human gastrointestinal tract, faces diverse transient conditions in different compartments upon oral ingestion. Genetic reporter systems have been demonstrated to be extremely powerful tools to ...
    Whole-genome Identification of Transcriptional Start Sites by Differential RNA-seq in Bacteria
    细菌转录起始位点的差异RNA序列全基因组鉴定
    作者:Ramón Cervantes-Rivera and Andrea Puhar日期:09/20/2020,浏览量:3097,Q&A: 0
    [Abstract] Gene transcription in bacteria often starts some nucleotides upstream of the start codon. Identifying the specific Transcriptional Start Site (TSS) is essential for genetic manipulation, as in many cases upstream of the start codon there are sequence elements that are involved in gene expression regulation. Taken into account the classical gene ...