系统生物学

分类

    现刊
    Differential Analysis of N-glycopeptide Abundance and N-glycosylation Site Occupancy for Studying Protein N-glycosylation Dysregulation in Human Disease
    用于研究人类疾病中蛋白质N-糖基化失调的N-糖肽丰度和N-糖基化位点的差异分析
    作者:Qi Zhang, Cheng Ma, Lian Li and Lih-Shen Chin日期:06/20/2021,浏览量:1069,Q&A: 0
    [Abstract]

    Protein N-glycosylation plays a vital role in diverse cellular processes, and dysregulated N-glycosylation is implicated in a variety of human diseases including neurodegenerative disorders and cancer. With recent advances in high-resolution mass spectrometry-based glycoproteomics technologies enabling large-scale N-glycoproteome profiling of

    ...
    BRIDGE: An Open Platform for Reproducible Protein-Ligand Simulations and Free Energy of Binding Calculations
    BRIDGE:一个可重复的蛋白质配体模拟和结合自由能计算的开放平台
    作者:Tharindu Senapathi, Christopher B. Barnett and Kevin J. Naidoo日期:09/05/2020,浏览量:5141,Q&A: 3
    [Abstract] Protein-ligand binding prediction is central to the drug-discovery process. This often follows an analysis of genomics data for protein targets and then protein structure discovery. However, the complexity of performing reproducible protein conformational analysis and ligand binding calculations, using vetted methods and protocols can be a ...
    A Protocol to Map the Spatial Proteome Using HyperLOPIT in Saccharomyces cerevisiae
    利用HyperLOPIT绘制酿酒酵母空间蛋白质组图谱的方法
    作者:Daniel J. H. Nightingale, Kathryn S. Lilley and Stephen G. Oliver日期:07/20/2019,浏览量:3612,Q&A: 0
    [Abstract] The correct subcellular localization of proteins is vital for cellular function and the study of this process at the systems level will therefore enrich our understanding of the roles of proteins within the cell. Multiple methods are available for the study of protein subcellular localization, including fluorescence microscopy, organelle ...
    Cell Type-specific Metabolic Labeling of Proteins with Azidonorleucine in Drosophila
    果蝇中含叠氮正亮氨酸蛋白质的细胞类型特异性代谢标记
    [Abstract] Advanced mass spectrometry technology has pushed proteomic analyses to the forefront of biological and biomedical research. Limitations of proteomic approaches now often remain with sample preparations rather than with the sensitivity of protein detection. However, deciphering proteomes and their context-dependent dynamics in subgroups of ...
    Character-State Reconstruction to Infer Ancestral Protein-Protein Interaction Patterns
    特征状态重建法推断遗传蛋白质相互作用模式
    作者:Florian Rümpler, Günter Theißen and Rainer Melzer日期:08/20/2015,浏览量:10905,Q&A: 0
    [Abstract] Protein-protein interactions are at the core of a plethora of developmental, physiological and biochemical processes. Consequently, insights into the origin and evolutionary dynamics of protein-protein interactions may provide information on the constraints and dynamics of specific biomolecular circuits and their impact on the organismal ...
    A Phosphopeptide Purification Protocol for the Moss Physcomitrella paten
    小立碗藓磷肽的纯化方法
    作者:Xiaoqin Wang and Yikun He日期:07/20/2015,浏览量:7870,Q&A: 0
    [Abstract] Protein phosphorylation is one of the most common post-translational modifications in eukaryotic cells and plays a critical role in a vast array of cellular processes. Efficient methods of protein extraction and phosphopeptide purification are required to ensure the detection of high quality of proteins. In our hands, phenol extraction of proteins ...
    Protocol for Preparation of Nuclear Protein from Mouse Lungs
    小鼠肺部细胞核蛋白制备法
    作者:Zuoxiang Xiao and Qun Jiang日期:06/05/2014,浏览量:12252,Q&A: 2
    [Abstract] This protocol describes how to extract nuclear protein from mouse lungs, including tissue preparation, stepwise lysis of cells and centrifugal isolation of nuclear protein fraction. This is an efficient method to get comparable nuclear protein extracts from lung tissues.
    Protein Sample Preparation for Proteomic Analysis in Leishmania donovani
    杜氏利什曼原虫中蛋白质组分析用蛋白质样本的制备
    作者:Alexandros Alexandratos and Despina Smirlis日期:03/05/2014,浏览量:9290,Q&A: 0
    [Abstract] Leishmania is a genus of trypanosomatid protozoa and is the parasite responsible for the disease leishmaniasis. These protozoa, regulate their gene expression in an atypical way, compared to other higher eukaryotes. The regulation of gene expression is characterized by a predominance of post-transcriptional over pre-transcriptional ...
    Isolation of the Secretome from Bacillus subtilis
    从枯草杆菌中分离分泌蛋白质组
    作者:Juri Niño Bach and Marc Bramkamp日期:01/20/2014,浏览量:9362,Q&A: 2
    [Abstract] Bacteria are commonly known to secret proteins in large amounts into the surrounding environment in high concentrations via various pathways. These proteins can be involved in numerous processes like cell-cell communication, exopolymer formation but also metabolic active enzymes are secreted that are interesting for industrial production of ...
    Analysis of Moraxella catarrhalis Outer Membrane Protein Profiles
    卡他莫拉菌外膜蛋白图谱分析
    作者:Stefan P.W. de Vries and Hester J. Bootsma日期:11/05/2013,浏览量:7757,Q&A: 0
    [Abstract] Phenotypes observed for certain Moraxella catarrhalis wild-type strains or mutants may be caused by a variety of factors including alteration of outer membrane protein composition. Examination of the outer membrane protein profiles may be a valuable tool to identify changes in outer membrane compositions of these strains. Here we describe ...