免疫学

分类

    现刊
    Isolation of Nasal Brush Cells for Single-cell Preparations
    用于单细胞制备的鼻刷细胞的分离
    作者:Saltanat Ualiyeva, Amelia A. Boyd, Nora A. Barrett and Lora G. Bankova日期:09/20/2021,浏览量:215,Q&A: 0
    [Abstract]

    Solitary chemosensory epithelial cells are scattered in most mucosal surfaces. They are referred to as tuft cells in the intestinal mucosa, brush cells in the trachea, and solitary chemosensory and microvillous cells in the nasal mucosa. They are the primary source of IL-25 in the epithelium and are also engaged in acetylcholine generation. We

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    Parasitemia Evaluation in Mice Infected with Schistosoma mansoni
    曼氏血吸虫感染小鼠的寄生虫病评估
    作者:Naiara Clemente Tavares and Marina Moraes Mourão日期:05/20/2021,浏览量:2154,Q&A: 0
    [Abstract]

    Schistosomiasis is a neglected tropical disease. Its treatment relies on the use of a single drug, praziquantel. Due to treatment limitations, an alternative for schistosomiasis chemotherapy is required; thus, a better understanding of parasite biology and host-parasite interactions is valuable to aid the identification of new anti-Schistosoma

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    In vivo CD40 Silencing by siRNA Infusion in Rodents and Evaluation by Kidney Immunostaining
    小鼠体内siRNA转染CD40沉默和肾免疫染色评价
    [Abstract]

    The co-stimulatory molecule CD40 and its ligand CD40L play a key role in the regulation of immunological processes and are involved in the pathophysiology of autoimmune and inflammatory diseases. Inhibition of the CD40-CD40L axis is a promising therapy, and a number of strategies and techniques have been designed to hinder its functionality. Our

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    Analysis of Monocyte Cell Fate by Adoptive Transfer in a Murine Model of TLR7-induced Systemic Inflammation
    TLR7诱导的系统性炎症小鼠模型中通过过继转移单核细胞命运分析
    作者:Jaba Gamrekelashvili, Hermann Haller and Florian P. Limbourg日期:05/05/2021,浏览量:2687,Q&A: 0
    [Abstract]

    Myeloid plasticity is a hallmark of the innate immune response to Toll-like receptor (TLR) activation. Here, we provide a protocol for monocyte cell fate tracking by adoptive transfer in the context of systemic inflammation induced by TLR7 activation, the principal innate immune receptor sensing viral RNA in mice. Defined monocyte subsets are

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    Using the Cecal Ligation and Puncture Model of Sepsis to Induce Rats to Multiple Organ Dysfunction
    采用败血症盲肠结扎穿刺模型诱导大鼠多脏器功能障碍
    [Abstract]

    Sepsis is a dysregulated hyperinflammatory disease caused by infection. Sepsis leads to multiple organ dysfunction syndrome (MODS), which is associated with high rates of mortality. The cecal ligation and puncture (CLP) model has been widely used in animals and has become the gold-standard method of replicating features of sepsis in humans.

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    Wounding Caenorhabditis elegans with Glass Wool
    玻璃棉用于秀丽隐杆线虫的创伤研究
    作者:Murugesan Pooranachithra, James Prabhanand Bhaskar and Krishnaswamy Balamurugan日期:01/20/2021,浏览量:1350,Q&A: 0
    [Abstract]

    Research on wound healing majorly relies on rat, mice and other animal models. However, an alternative animal model ought to be brought in the field, pertaining to the stringent ethical issues owing to the use of animals in research. In this regard, Caenorhabdits elegans, a miniature model nematode gains the great attention of the researchers in

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    Preparation of Nippostrongylus brasiliensis Larvae for the Study of Host Skin Response
    用于研究寄主皮肤反应的巴西日圆线虫幼虫的制备
    作者:Tiffany Bouchery, Gillian Coakley and Nicola L. Harris日期:12/20/2020,浏览量:924,Q&A: 0
    [Abstract]

    Hookworms are skin penetrating parasites, however in the laboratory the hookworm model Nippostrongylus brasiliensis, the parasite is traditionally administered subcutaneously bypassing the skin (epidermis and dermis). Here, we describe two complementary approaches for infecting mice with N. brasiliensis in order to study the skin immune responses.

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    Pea Aphid Rearing, Bacterial Infection and Hemocyte Phagocytosis Assay
    豌豆蚜虫饲养、细菌感染及血细胞吞噬功能测定
    作者:Li Ma, Lu Liu and Zhiqiang Lu日期:12/20/2020,浏览量:1181,Q&A: 0
    [Abstract]

    Insects rely on the simple but effective innate immune system to combat infection. Cellular and humoral responses are interconnected and synergistic in insects’ innate immune system. Phagocytosis is one major cellular response. It is difficult to collect clean hemolymph from the small insect like pea aphid. Here, we provide a practicable

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    Maternal Immune Activation with the Viral Mimetic Poly:IC in Pregnant Rats
    Poly:IC模拟病毒感染对孕鼠的母体免疫激活
    作者:Thaisa Meira Sandini, Quentin Greba, Brittney Rose Lins and John George Howland日期:11/20/2020,浏览量:1299,Q&A: 0
    [Abstract]

    Maternal immune activation (MIA) is increasingly well appreciated as an environmental risk factor for some psychiatric disorders. Administration of proinflammatory compounds such as the synthetic double-stranded RNA molecule polyinosinic-polycytidylic acid (polyI:C) to pregnant rodents results in the release of proinflammatory cytokines in the

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    Surgical Induction of Endometriosis in Female Mice
    雌性小鼠子宫内膜异位症的手术诱导
    作者:Alejandra Escudero-Lara, David Cabañero and Rafael Maldonado日期:09/20/2020,浏览量:1637,Q&A: 0
    [Abstract] Endometriosis is a common gynecological disease characterized by the presence of endometrial tissue outside the uterine cavity. It is frequently associated with pain, infertility and a reduced quality of life, and it lacks adequate treatment. Several rodent models of endometriosis have been developed through heterologous and homologous ...