系统生物学

分类

    现刊
    Protocols to Induce, Prevent, and Treat Post-traumatic Stress Disorder-like Memory in Mice: Optogenetics and Behavioral Approaches
    诱导、预防和治疗小鼠创伤后应激障碍样记忆的方案:光遗传学和行为方法
    作者:Aline S. Al Abed, Azza Sellami, Eva-Gunnel Ducourneau, Chloé Bouarab, Aline Marighetto and Aline Desmedt日期:10/05/2021,浏览量:355,Q&A: 0
    [Abstract]

    One of the cardinal features of post-traumatic stress disorder (PTSD) is a paradoxical memory alteration including both emotional hypermnesia for salient trauma-related cues and amnesia for the surrounding traumatic context. Interestingly, some clinical studies have suggested that contextual amnesia would causally contribute to the PTSD-related

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    Protocol for RNA-seq Expression Analysis in Yeast
    酵母RNA-seq表达分析方法
    作者:Stefan Bohn日期:09/20/2021,浏览量:526,Q&A: 0
    [Abstract]

    Genome-wide sequencing of RNA (RNA-seq) has become an inexpensive tool to gain key insights into cellular and disease mechanisms. Sample preparation and sequencing are streamlined and allow the acquisition of hundreds of gene expression profiles in a few days; however, in particular, data processing, curation, and analysis involve numerous steps

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    Detecting Differentially Methylated Promoters in Genes Related to Disease Phenotypes Using R
    利用R检测与疾病表型相关基因的差异甲基化启动子
    作者:Jordi Martorell Marugán and Pedro Carmona-Sáez日期:06/05/2021,浏览量:1313,Q&A: 0
    [Abstract]

    DNA methylation in gene promoters plays a major role in gene expression regulation, and alterations in methylation patterns have been associated with several diseases. In this context, different software suites and statistical methods have been proposed to analyze differentially methylated positions and regions. Among them, the novel statistical

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    Identification of R-loop-forming Sequences in Drosophila melanogaster Embryos and Tissue Culture Cells Using DRIP-seq
    DRIP-seq法鉴定黑腹果蝇胚胎和组织培养细胞R-loop形成序列
    作者:Célia Alecki and Nicole J. Francis日期:05/05/2021,浏览量:3076,Q&A: 0
    [Abstract]

    R-loops are non-canonical nucleic structures composed of an RNA–DNA hybrid and a displaced ssDNA. Originally identified as a source of genomic instability, R-loops have been shown over the last decade to be involved in the targeting of proteins and to be associated with different histone modifications, suggesting a regulatory function. In

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    Computational Analysis and Phylogenetic Clustering of SARS-CoV-2 Genomes
    SARS-CoV-2基因组计算分析及系统进化聚类分析
    作者:Bani Jolly and Vinod Scaria日期:04/20/2021,浏览量:2847,Q&A: 0
    [Abstract]

    COVID-19, the disease caused by the novel SARS-CoV-2 coronavirus, originated as an isolated outbreak in the Hubei province of China but soon created a global pandemic and is now a major threat to healthcare systems worldwide. Following the rapid human-to-human transmission of the infection, institutes around the world have made efforts to generate

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    Reference-free Association Mapping from Sequencing Reads Using k-mers
    利用k-mers进行测序读取的无参考关联映射
    作者:Zakaria Mehrab, Jaiaid Mobin, Ibrahim Asadullah Tahmid, Lior Pachter and Atif Rahman日期:11/05/2020,浏览量:2907,Q&A: 0
    [Abstract] Association mapping is the process of linking phenotypes with genotypes. In genome wide association studies (GWAS), individuals are first genotyped using microarrays or by aligning sequenced reads to reference genomes. However, both these approaches rely on reference genomes which limits their application to organisms with no or incomplete ...
    Whole-genome Identification of Transcriptional Start Sites by Differential RNA-seq in Bacteria
    细菌转录起始位点的差异RNA序列全基因组鉴定
    作者:Ramón Cervantes-Rivera and Andrea Puhar日期:09/20/2020,浏览量:3097,Q&A: 0
    [Abstract] Gene transcription in bacteria often starts some nucleotides upstream of the start codon. Identifying the specific Transcriptional Start Site (TSS) is essential for genetic manipulation, as in many cases upstream of the start codon there are sequence elements that are involved in gene expression regulation. Taken into account the classical gene ...
    HoSeIn: A Workflow for Integrating Various Homology Search Results from Metagenomic and Metatranscriptomic Sequence Datasets
    HoSeIn:整合来自宏基因组和元转录组序列数据集的各种同源性搜索结果的工作流
    作者:Gaston Rozadilla, Jorgelina Moreiras Clemente and Christina B. McCarthy日期:07/20/2020,浏览量:2357,Q&A: 0
    [Abstract] Data generated by metagenomic and metatranscriptomic experiments is both enormous and inherently noisy. When using taxonomy-dependent alignment-based methods to classify and label reads, the first step consists in performing homology searches against sequence databases. To obtain the most information from the samples, nucleotide sequences are ...
    Delayed Alternation Task for the Study of Spatial Working and Long Term Memory in Rats
    延迟交替任务用于研究大鼠空间工作记忆和长期记忆
    作者:Megi Hoxha and Marta Sabariego日期:03/05/2020,浏览量:2956,Q&A: 0
    [Abstract] Memory systems can hold previously presented information for several seconds, bridging gaps between discontinuous events. It has been previously demonstrated that the hippocampus and the medial entorhinal cortex (mEC) are necessary for memory retention over delay intervals in alternation tasks. Here we describe the delayed alternation task, a ...
    ChIP-Seq from Limited Starting Material of K562 Cells and Drosophila Neuroblasts Using Tagmentation Assisted Fragmentation Approach
    用Tagmentation Assisted Fragmentation法从K562细胞和果蝇神经母细胞的有限起始材料中提取芯片序列
    作者:Junaid Akhtar, Piyush More and Steffen Albrecht日期:02/20/2020,浏览量:2170,Q&A: 0
    [Abstract] Chromatin immunoprecipitation is extensively used to investigate the epigenetic profile and transcription factor binding sites in the genome. However, when the starting material is limited, the conventional ChIP-Seq approach cannot be implemented. This protocol describes a method that can be used to generate the chromatin profiles from as low as ...