系统生物学

分类

    现刊
    Trypanosomatid, fluorescence-based in vitro U-insertion/U-deletion RNA-editing (FIDE)
    基于荧光的锥虫体体外尿嘧啶插入或删除RNA编辑(FIDE)
    作者:Wolf-Matthias Leeder, Elisabeth Kruse and H. Ulrich Göringer日期:03/05/2021,浏览量:684,Q&A: 0
    [Abstract]

    Gene expression within the mitochondria of African trypanosomes and other protozoan organisms relies on a nucleotide-specific RNA-editing reaction. In the process exclusively uridine (U)-nucleotides are site-specifically inserted into and deleted from sequence-deficient primary transcripts to convert them into translatable mRNAs. The reaction is

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    Primer ID Next-Generation Sequencing for the Analysis of a Broad Spectrum Antiviral Induced Transition Mutations and Errors Rates in a Coronavirus Genome
    用于分析冠状病毒基因组中广谱抗病毒药物诱导的过渡突变和错误率的引物ID下一代测序
    作者:Shuntai Zhou, Collin S. Hill, Michael U. Clark, Timothy P. Sheahan, Ralph Baric and Ronald Swanstrom日期:03/05/2021,浏览量:1577,Q&A: 0
    [Abstract]

    Next generations sequencing (NGS) has become an important tool in biomedical research. The Primer ID approach combined with the MiSeq platform overcomes the limitation of PCR errors and reveals the true sampling depth of population sequencing, making it an ideal tool to study mutagenic effects of potential broad-spectrum antivirals on RNA viruses.

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    EmPC-seq: Accurate RNA-sequencing and Bioinformatics Platform to Map RNA Polymerases and Remove Background Error
    EmPC-seq: 使用精确的RNA测序和生物信息学平台绘制RNA聚合酶并消除背景错误
    [Abstract]

    Transcription errors can substantially affect metabolic processes in organisms by altering the epigenome and causing misincorporations in mRNA, which is translated into aberrant mutant proteins. Moreover, within eukaryotic genomes there are specific Transcription Error-Enriched genomic Loci (TEELs) which are transcribed by RNA polymerases with

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    Low-cost and High-throughput RNA-seq Library Preparation for Illumina Sequencing from Plant Tissue
    植物组织中用于Illumina测序的低成本、高通量RNA-seq文库制备
    作者:Marta Bjornson, Kaisa Kajala, Cyril Zipfel and Pingtao Ding日期:10/20/2020,浏览量:2068,Q&A: 0
    [Abstract] Transcriptome analysis can provide clues to biological processes affected in different genetic backgrounds or/and under various conditions. The price of RNA sequencing (RNA-seq) has decreased enough so that medium- to large-scale transcriptome analyses in a range of conditions are feasible. However, the price and variety of options for library ...
    Low-cost and Multiplexable Whole mRNA-Seq Library Preparation Method with Oligo-dT Magnetic Beads for Illumina Sequencing Platforms
    Oligo-dT磁珠用于Illumina测序平台的低成本和可复用的完整mRNA-Seq文库制备方法
    作者:Makoto Kashima, Ayumi Deguchi, Ayumi Tezuka and Atsushi J. Nagano日期:06/20/2020,浏览量:3045,Q&A: 0
    [Abstract] RNA-Seq is a powerful method for transcriptome analysis used in varied field of biology. Although several commercial products and hand-made protocols enable us to prepare RNA-Seq library from total RNA, their cost are still expensive. Here, we established a low-cost and multiplexable whole mRNA-Seq library preparation method for illumine ...
    HIV-CRISPR: A CRISPR/Cas9 Screening Method to Identify Genes Affecting HIV Replication
    HIV-CRISPR:一种CRISPR/Cas9筛选HIV复制相关基因的方法
    作者:Ferdinand Roesch and Molly OhAinle日期:05/05/2020,浏览量:3877,Q&A: 0
    [Abstract] Screening with CRISPR/Cas9 technology has already led to significant discoveries in the fields of cancer biology, cell biology and virology. Because of the relatively low false discovery rates and the ability to perform high-throughput, pooled approaches, it has rapidly become the assay of choice for screening studies, including whole-genome ...
    Sequence Alignment Using Machine Learning for Accurate Template-based Protein Structure Prediction
    基于模板的蛋白质结构精确预测的机器学习序列比对
    作者:Shuichiro Makigaki and Takashi Ishida日期:05/05/2020,浏览量:2399,Q&A: 0
    [Abstract] Template-based modeling, the process of predicting the tertiary structure of a protein by using homologous protein structures, is useful when good templates can be available. Indeed, modern homology detection methods can find remote homologs with high sensitivity. However, the accuracy of template-based models generated from the ...
    Circadian Gene Profiling in Laser Capture Microdissected Mouse Club Cells
    激光捕获显微解剖小鼠棒状细胞的昼夜节律基因分析
    作者:Zhenguang Zhang and Andrew Loudon日期:04/20/2020,浏览量:1582,Q&A: 0
    [Abstract] Cell heterogeneity is high in tissues like lung. Research conducted on pure population of cells usually offers more insights than bulk tissues, such as circadian clock work. In this protocol, we provide a detailed work flow on how to do circadian clock study by RNA seq in laser capture micro-dissected mouse lung club cells. The method uses frozen ...
    mRNA Extraction from Gill Tissue for RNA-sequencing
    鳃组织mRNA 提取用于RNA测序
    作者:Jukka-Pekka Verta and Felicity Jones日期:03/05/2020,浏览量:2273,Q&A: 0
    [Abstract] Adaptation is thought to proceed in part through spatial and temporal changes in gene expression. Fish species such as the threespine stickleback are powerful vertebrate models to study the genetic architecture of adaptive changes in gene expression since divergent adaptation to different environments is common, they are abundant and easy to study ...
    Adapting the Smart-seq2 Protocol for Robust Single Worm RNA-seq
    改编Smart-seq2实验方案以实现稳定的单蠕虫RNA测序
    [Abstract] Most nematodes are small worms that lack enough RNA for regular RNA-seq protocols without pooling hundred to thousand of individuals. We have adapted the Smart-seq2 protocol in order to sequence the transcriptome of an individual worm. While developed for individual Steinernema carpocapsae and Caenorhabditis elegans larvae as ...