神经科学

分类

    现刊
    Enrichment of Vascular Fragments from Mouse Embryonic Brains for Endothelial Cell Analysis
    用于内皮细胞分析小鼠胚胎脑血管片段富集
    作者:Nicolas Santander and Thomas D. Arnold日期:06/20/2021,浏览量:1072,Q&A: 0
    [Abstract]

    Endothelial cells in the brain interact with other cell types, forming the blood-brain barrier. This barrier controls the movement of solutes into and out of the brain, regulating pathophysiological processes and drug delivery to the brain. Common isolation methods used to study these cells during embryonic development involve enzymatic treatment

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    Ex vivo Tissue Culture Protocols for Studying the Developing Neocortex
    研究发育中的新皮质的体外组织培养方案
    作者:Takashi Namba, Christiane Haffner and Wieland B. Huttner日期:05/20/2021,浏览量:1867,Q&A: 0
    [Abstract]

    The size of the neocortex and its morphology are highly divergent across mammalian species. Several approaches have been utilized for the analysis of neocortical development and comparison among different species. In the present protocol (Note: This protocol requires basic knowledge of brain anatomy), we describe three ex vivo neocortical

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    Development of a Chemical Reproductive Aging Model in Female Rats
    雌性大鼠化学生殖衰老模型的建立
    [Abstract]

    Women are born with an abundant but finite pool of ovarian follicles, which naturally and progressively decreased during their reproductive years until menstrual periods stop permanently (menopause). Perimenopause represents the transition from reproductive to non-reproductive life. It is usually characterized by neuroendocrine, metabolic and

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    Ligand and Carbohydrate Engagement (LACE) Assay and Fluorescence Quantification on Murine Neural Tissue
    小鼠神经组织中配体和碳水化合物结合(LACE)实验和荧光定量测定
    作者:James M. Clegg and Thomas Pratt日期:03/20/2021,浏览量:2206,Q&A: 0
    [Abstract]

    The interaction between cell surface heparan sulphate and diffusible ligands such as FGFs is of vital importance for downstream signaling, however, there are few techniques that can be used to investigate this binding event. The ligand and carbohydrate engagement (LACE) assay is a powerful tool which can be used to probe the molecular interaction

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    Optimized Immunostaining of Embryonic and Early Postnatal Mouse Brain Sections
    胚胎和产后早期小鼠脑切片的优化免疫染色
    作者:Kawssar Harb, Michele Bertacchi and Michèle Studer日期:01/05/2021,浏览量:1745,Q&A: 0
    [Abstract]

    The mammalian neocortex, the outer layer of the cerebrum and most recently evolved brain region, is characterized by its unique areal and laminar organization. Distinct cortical layers and areas can be identified by the protein expression of graded transcription factors and molecular determinants that define the identity of different projection

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    A Parkinson’s Disease-relevant Mitochondrial and Neuronal Morphology High-throughput Screening Assay in LUHMES Cells
    LUHMES细胞中与帕金森氏病相关的线粒体和神经元形态学高通量筛选分析
    作者:Tom Leah, Irina Vazquez-Villaseñor, Laura Ferraiuolo, Stephen B Wharton and Heather Mortiboys日期:01/05/2021,浏览量:1960,Q&A: 0
    [Abstract]

    Parkinson’s disease is a devastating neurodegenerative disorder affecting 2-3% of the population over 65 years of age. There is currently no disease-modifying treatment. One of the predominant pathological features of Parkinson’s disease is mitochondrial dysfunction, and much work has aimed to identify therapeutic compounds which can

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    Whole-mount Immunohistochemistry of Adult Zebrafish Retina for Advanced Imaging
    用于图像采集的成年斑马鱼视网膜的整体免疫组化研究
    作者:Mikiko Nagashima and Peter F. Hitchcock日期:12/20/2020,浏览量:1673,Q&A: 0
    [Abstract]

    Immunohistochemistry is a widely used technique to examine the expression and subcellular localization of proteins. This technique relies on the specificity of antibodies and requires adequate penetration of antibodies into tissues. The latter is especially challenging for thick specimens, such as embryos and other whole-mount preparations. Here

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    Staining and Quantitative Analysis of Myelinating Oligodendrocytes in the Mouse Grey Matter
    小鼠灰质髓鞘少突胶质细胞的染色及定量分析
    作者:Matthew Swire and Charles ffrench-Constant日期:10/20/2020,浏览量:1512,Q&A: 0
    [Abstract] Oligodendrocytes generate distinct patterns of myelination throughout the CNS. Variations in myelination along axons may enable neurons to fine-tune conduction velocities and alter signal synchronisation. Here we outline a staining protocol permitting the assessment of the number and length of myelin sheaths formed by oligodendrocyte in the mouse ...
    Organotypic Slice Culture of the Embryonic Mouse Brain
    胚胎小鼠大脑的器官型切片培养
    作者:James M. Clegg and Thomas Pratt日期:07/05/2020,浏览量:1720,Q&A: 0
    [Abstract] Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays and bead culture assays.
    Rapid Generation of Human Neuronal Cell Models Enabling Inducible Expression of Proteins-of-interest for Functional Studies
    快速建立能诱导表达功能研究所需蛋白的人神经细胞模型
    [Abstract] CRISPR-Cas9 technology has transformed the ability to edit genomic sequences and control gene expression with unprecedented ease and scale. However, precise genomic insertions of coding sequences using this technology remain time-consuming and inefficient because they require introducing adjacent single-strand cuts through Cas9 nickase action and ...