神经科学

分类

    现刊
    Differential Analysis of N-glycopeptide Abundance and N-glycosylation Site Occupancy for Studying Protein N-glycosylation Dysregulation in Human Disease
    用于研究人类疾病中蛋白质N-糖基化失调的N-糖肽丰度和N-糖基化位点的差异分析
    作者:Qi Zhang, Cheng Ma, Lian Li and Lih-Shen Chin日期:06/20/2021,浏览量:0,Q&A: 0
    [Abstract]

    Protein N-glycosylation plays a vital role in diverse cellular processes, and dysregulated N-glycosylation is implicated in a variety of human diseases including neurodegenerative disorders and cancer. With recent advances in high-resolution mass spectrometry-based glycoproteomics technologies enabling large-scale N-glycoproteome profiling of

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    A Lipidomics Approach to Measure Phosphatidic Acid Species in Subcellular Membrane Fractions Obtained from Cultured Cells
    用脂质组学方法测定培养细胞亚细胞膜组分中磷脂酸的种类
    作者:Nawal Kassas, Laetitia Fouillen, Stéphane Gasman and Nicolas Vitale日期:06/20/2021,浏览量:0,Q&A: 0
    [Abstract]

    Over the last decade, lipids have emerged as possessing an ever-increasing number of key functions, especially in membrane trafficking. For instance, phosphatidic acid (PA) has been proposed to play a critical role in different steps along the secretory pathway or during phagocytosis. To further investigate in detail the precise nature of PA

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    Dual Color, Live Imaging of Vesicular Transport in Axons of Cultured Sensory Neurons
    培养感觉神经元轴突囊泡运输的双色实时成像
    作者:Yoko Bekku and James L. Salzer日期:06/20/2021,浏览量:89,Q&A: 0
    [Abstract]

    The function of neurons in afferent reception, integration, and generation of electrical activity relies on their strikingly polarized organization, characterized by distinct membrane domains. These domains have different compositions resulting from a combination of selective targeting and retention of membrane proteins. In neurons, most proteins

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    In vivo Fluorescence Imaging of Extracellular ATP in the Mouse Cerebral Cortex with a Hybrid-type Optical Sensor
    混合型光传感器对小鼠大脑皮层细胞外ATP的体内荧光成像
    [Abstract]

    Adenosine 5’-triphosphate (ATP) works as an extracellular signaling molecule for cells in the brain, such as neurons and glia. Cellular communication via release of ATP is involved in a range of processes required for normal brain functions, and aberrant communication is associated with brain disorders. To investigate the mechanisms

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    A New Method for Studying RNA-binding Proteins on Specific RNAs
    一种研究特异性RNA结合蛋白的新方法
    作者:Weiping Sun, Ziheng Zhang, Ji-Long Liu and Min Zhuang日期:05/20/2021,浏览量:1362,Q&A: 0
    [Abstract]

    Proximity-based protein labeling has been developed to identify protein-nucleic acid interactions. We have reported a novel method termed CRUIS (CRISPR-based RNA-United Interacting System), which captures RNA-protein interactions in living cells by combining the RNA-binding capacity of CRISPR/Cas13 and the proximity-tagging activity of PUP-IT.

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    Imaging Microtubules in vitro at High Resolution while Preserving their Structure
    保留微管结构同时在体外对其进行高分辨率成像
    作者:Camille Cuveillier, Yasmina Saoudi, Isabelle Arnal and Christian Delphin日期:04/05/2021,浏览量:529,Q&A: 0
    [Abstract]

    Microtubules (MT) are the most rigid component of the cytoskeleton. Nevertheless, they often appear highly curved in the cellular context and the mechanisms governing their overall shape are poorly understood. Currently, in vitro microtubule analysis relies primarily on electron microscopy for its high resolution and Total Internal Reflection

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    Investigate Synaptic Vesicles Mobility in Neuronal Culture Axons by FRAP Imaging
    用FRAP成像技术研究神经元轴突中突触囊泡的移动
    作者:Xiao Min Zhang, Fabrice P. Cordelieres and Etienne Herzog日期:03/20/2021,浏览量:1026,Q&A: 0
    [Abstract]

    Synaptic vesicles (SVs) are clustered in the presynaptic terminals and consistently trafficking along axons. Based on their release features, SVs are classified into different “pools”. Imaging of SVs that are traveling among multiple presynaptic terminals has helped define a new pool named “SV super-pool”. Here we describe

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    Generation of Mouse Primary Hypothalamic Neuronal Cultures for Circadian Bioluminescence Assays
    培养小鼠原发性下丘脑神经元用于昼夜生物发光分析
    作者:Cosima X. Schmidt, Anthony H. Tsang and Henrik Oster日期:03/05/2021,浏览量:674,Q&A: 0
    [Abstract]

    An endogenous circadian clock system enables organisms to adapt to time-of-day dependent environmental changes. In consequence, most physiological processes exhibit daily rhythms of, e.g., energy metabolism, immune function, sleep, or hormone production. Hypothalamic circadian clocks have been identified to play a particular role in coordinating

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    In vitro Measurement of Membrane Attack Complex in RPE Cells
    视网膜色素上皮细胞膜攻击复合物的体外测定
    作者:Kelly Mulfaul and Sarah L. Doyle日期:02/20/2021,浏览量:962,Q&A: 0
    [Abstract]

    Initiation of the complement system results in the formation of a multiprotein pore termed the membrane attack complex (MAC, C5b-C9). MAC pores accumulate on a cell surface and can result in cell lysis. The retinal pigment epithelium (RPE) is a single monolayer of pigmented epithelial cells located at the posterior poll of the eye that forms the

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    Immuno-electrophysiology on Neuromuscular Junctions of Drosophila Third Instar Larva
    果蝇三龄幼虫神经肌肉连接的免疫电生理研究
    作者:Raffaella Klima, Giulia Romano, Monsurat Gbadamosi, Aram Megighian and Fabian Feiguin日期:02/05/2021,浏览量:670,Q&A: 0
    [Abstract]

    Alterations in synaptic transmission are critical early events in neuromuscular disorders. However, reliable methodologies to analyze the functional organization of the neuromuscular synapses are still needed. This manuscript provides a detailed protocol to analyze the molecular assembly of the neuromuscular synapses through

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