神经科学

分类

    现刊
    Measurement of LRRK2 Kinase Activity by Proximity Ligation Assay
    通过邻近连接测定法测量 LRRK2 激酶活性
    作者:Matthew T. Keeney, Eric K. Hoffman, J. Timothy Greenamyre and Roberto Di Maio日期:09/05/2021,浏览量:505,Q&A: 0
    [Abstract]

    Missense mutations in leucine rich-repeat kinase 2 (LRRK2) cause forms of familial Parkinson’s disease and have been linked to ‘idiopathic’ Parkinson’s disease. Assessment of LRRK2 kinase activity has been very challenging due to its size, complex structure, and relatively low abundance. A standard in the field to assess

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    Unraveling the Physicochemical Determinants of Protein Liquid-liquid Phase Separation by Nanoscale Infrared Vibrational Spectroscopy
    利用纳米尺度红外振动光谱揭示蛋白质液-液相分离的理化决定因素
    作者:Francesco S. Ruggeri, Alyssa M. Miller, Michele Vendruscolo and Tuomas P. J. Knowles日期:08/20/2021,浏览量:937,Q&A: 0
    [Abstract]

    The phenomenon of reversible liquid-liquid phase separation of proteins underlies the formation of membraneless organelles, which are crucial for cellular processes such as signalling and transport. In addition, it is also of great interest to uncover the mechanisms of further irreversible maturation of the functional dense liquid phase into

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    A Simple Spatial-independent Associative and Reversal Learning Task in Mice
    小鼠的一个简单的空间独立的联想和逆转学习任务
    作者:Fulvio Magara, Benjamin Boury-Jamot and Hanna Hörnberg日期:08/05/2021,浏览量:573,Q&A: 0
    [Abstract]

    The ability to adapt one's behavior in response to changing circumstances, or cognitive flexibility, is often altered in neuropsychiatric and neurodevelopmental conditions. In rodents, cognitive flexibility is frequently assessed using associative learning paradigms with a reversal component. The majority of existing protocols rely on

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    Evaluating Baseline and Sensitised Heat Nociception in Adult Drosophila
    评估成年果蝇的基线和致敏热伤害感受
    作者:Josephine N. Massingham, Olga Baron and G. Gregory Neely日期:07/05/2021,浏览量:771,Q&A: 0
    [Abstract]

    Chronic pain is a complex disease that affects a large proportion of the population. With little to no effective treatments currently available for patients, this malady presents a large burden to society. Drosophila melanogaster has been previously used to describe conserved molecular components of nociception in larvae and adults. However, adult

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    Intravenous and Non-invasive Drug Delivery to the Mouse Basal Forebrain Using MRI-guided Focused Ultrasound
    MRI引导下聚焦超声对小鼠基底前脑静脉及无创给药影响的研究
    作者:Kristiana Xhima, Dallan McMahon, Edward Ntiri, Maged Goubran, Kullervo Hynynen and Isabelle Aubert日期:06/20/2021,浏览量:1473,Q&A: 1
    [Abstract]

    Basal forebrain cholinergic neurons (BFCNs) regulate circuit dynamics underlying cognitive processing, including attention, memory, and cognitive flexibility. In Alzheimer’s disease and related neurodegenerative conditions, the degeneration of BFCNs has long been considered a key player in cognitive decline. The cholinergic system thus

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    Operant Vapor Self-administration in Mice
    小鼠蒸汽吸入自我给药操作
    作者:Renata C. N. Marchette, Brendan J. Tunstall, Leandro F. Vendruscolo and Khaled Moussawi日期:05/20/2021,浏览量:1168,Q&A: 0
    [Abstract]

    Models of drug addiction in rodents are instrumental in understanding the underlying neurobiology. Intravenous self-administration of drugs in mice is currently the most commonly used model; however, several challenges exist due to complications related to catheter patency. To take full advantage of the genetic tools available to study opioid

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    Activity-based Anorexia for Modeling Vulnerability and Resilience in Mice
    基于活动的厌食症小鼠脆弱性和恢复力模型的建立
    作者:Jeff A. Beeler and Nesha S. Burghardt日期:05/05/2021,浏览量:633,Q&A: 0
    [Abstract]

    Activity-based anorexia (ABA) is a widely used rodent model of anorexia nervosa. It involves combining limited access to food with unlimited access to a running wheel, leading to a paradoxical decrease in food intake, hyperactivity, and life-threatening weight loss. Although initially characterized in rats, ABA has been tested in mice with results

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    Generation of Human iPSC-derived Neural Progenitor Cells (NPCs) as Drug Discovery Model for Neurological and Mitochondrial Disorders
    人来源iPSC获得的神经祖细胞(NPCs)的产生作为神经和线粒体疾病的药物发现模型
    作者:Annika Zink, Pawel Lisowski and Alessandro Prigione日期:03/05/2021,浏览量:2568,Q&A: 1
    [Abstract]

    The high attrition rate in drug development processes calls for additional human-based model systems. However, in the context of brain disorders, sampling live neuronal cells for compound testing is not applicable. The use of human induced pluripotent stem cells (iPSCs) has revolutionized the field of neuronal disease modeling and drug discovery.

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    Relative Quantification of NaV1.1 Protein in Mouse Brains Using a Meso Scale Discovery-Electrochemiluminescence (MSD-ECL) Method
    利用中尺度发现电化学发光(MSD-ECL)方法对小鼠脑中NaV1.1蛋白进行相对定量
    作者:Zhou Han, Anne Christiansen, Meena and Gene Liau日期:02/05/2021,浏览量:1044,Q&A: 0
    [Abstract]

    Densitometric analysis is often used to quantify NaV1.1 protein on immunoblots, although the sensitivity and dilution linearity of the method are usually poor. Here we present a protocol for quantification of NaV1.1 in mouse brain tissues using a Meso Scale Discovery-Electrochemiluminescence (MSD-ECL) method. MSD-ECL is based on ELISA

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    Characterization of Hippocampal Adult-borne Granule Cells in a Transient Cerebral Ischemia Model
    海马成体新生颗粒细胞在短暂性脑缺血模型中的特征
    作者:Mihai Ceanga, Madlen Guenther, Ina Ingrisch and Albrecht Kunze日期:01/20/2021,浏览量:907,Q&A: 0
    [Abstract]

    Long-term consequences of stroke significantly impair the quality of life in a growing population of stroke survivors. Hippocampal adult neurogenesis has been hypothesized to play a role in the pathophysiology of cognitive and neuropsychiatric long-term sequelae of stroke. Reliable animal models of stroke are paramount to understanding their

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