干细胞

分类

    现刊
    A Simple Method to Generate Super-sensitive AID (ssAID)-based Conditional Knockouts using CRISPR-based Gene Knockout in Various Vertebrate Cell Lines
    小胶质细胞的分离及蛋白表达的流式细胞仪分析:避免小胶质细胞背景荧光的陷阱
    作者:Kohei Nishimura and Tatsuo Fukagawa日期:07/20/2021,浏览量:389,Q&A: 0
    [Abstract]

    Inducing loss of function of a target protein using methods such as gene knockout is a powerful and useful strategy for analyzing protein function in cells. In recent years, the CRISPR/Cas-9-based gene knockout technology has been widely used across a variety of eukaryotes; however, this type of simple gene knockout strategy is not applicable to

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    Generation of Mouse Pluripotent Stem Cell-derived Trunk-like Structures: An in vitro Model of Post-implantation Embryogenesis
    小鼠多能干细胞衍生干细胞样结构的生成:植入术后胚胎发生的体外模型
    [Abstract]

    Post-implantation mammalian embryogenesis involves profound molecular, cellular, and morphogenetic changes. The study of these highly dynamic processes is complicated by the limited accessibility of in utero development. In recent years, several complementary in vitro systems comprising self-organized assemblies of mouse embryonic stem cells, such

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    A Modified Semisolid Clonal Culture for Identification of B-1 and B-2 Progenitor Colony Forming Ability of Mouse Embryonic Hemogenic Endothelial Cells
    改良半固态克隆培养鉴定小鼠胚胎造血内皮细胞B-1和B-2祖细胞集落形成能力
    作者:Michihiro Kobayashi and Momoko Yoshimoto日期:05/05/2020,浏览量:1640,Q&A: 0
    [Abstract] The search for the origin of the first hematopoietic stem cells (HSCs) in the mouse embryo has been a hot topic in the field of developmental hematopoiesis. Detecting lymphoid potential is one of the supportive evidence to show the definitive hematopoietic activity of HSCs. However, the first B-lymphoid potential in the mouse embryos are reported ...
    Differentiation of Mouse Embryonic Stem Cells to Neuronal Cells Using Hanging Droplets and Retinoic Acid
    利用悬滴和维甲酸诱导小鼠胚胎干细胞向神经元细胞的分化
    作者:Jeroen Witteveldt and Sara Macias日期:11/05/2019,浏览量:3247,Q&A: 0
    [Abstract] Controlled differentiation of embryonic stem cells is an essential tool in stem cell research. In this protocol, we describe a simple differentiation protocol involving the induction of embryoid body formation in mouse embryonic stem cells (mESC) using hanging droplets, followed by differentiation into a neuronal lineage.
    An Improved Staining Method for Low Signal LacZ Reporter Detection in Mouse Embryos
    一种改进的可用于检测小鼠胚胎弱LacZ报告信号的染色法
    作者:Xiaopeng Shen, Feng Xu, Shen Wu, Meng Li, Jingyi Zhang, Rui Liang and Yu Liu日期:03/05/2019,浏览量:4119,Q&A: 0
    [Abstract] In many fields of biology, especially in the field of developmental biology, LacZ reporter staining is an approach used to monitor gene expression patterns. In the LacZ reporter system, the LacZ gene is inserted in the endogenous location of the target gene via gene knock-in or by constructing a transgenic cassette in ...
    Isolation of Neural Stem Cells from the Embryonic Mouse Hippocampus for in vitro Growth or Engraftment into a Host Tissue
    从胚胎小鼠海马分离神经干细胞用于体外生长或植入宿主组织
    作者:Oksana Rybachuk, Olga Kopach, Tetyana Pivneva and Vitaliy Kyryk日期:02/20/2019,浏览量:5432,Q&A: 0
    [Abstract] For both stem cell research and treatment of the central nervous system disorders, neural stem/progenitor cells (NSPCs) represent an important breakthrough tool. In the expanded stem cell-based therapy use, NSPCs not only provide a powerful cell source for neural cell replacement but a useful model for developmental biology research. Despite ...
    Quantification of Mouse Hematopoietic Progenitors’ Formation Using Time-lapse Microscopy and Image Analysis
    使用延时显微技术和图像分析定量小鼠造血祖细胞的形成
    作者:Isabelle Bergiers, Christian Tischer, Özge Vargel Bölükbaşı and Christophe Lancrin日期:01/05/2019,浏览量:3988,Q&A: 0
    [Abstract] In vitro differentiation of mouse embryonic stem cells (mESCs) towards blood cells constitutes a well-established system to study the endothelial-to-hematopoietic transition (EHT) at the onset of blood development. Assessing the emergence of small non-adherent round blood cells in the culture without disturbing it is essential to evaluate ...
    Small Molecule-Based Retinal Differentiation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
    基于小分子的人胚胎干细胞和诱导多能干细胞的视网膜分化
    作者:Jie Zhu and Deepak A. Lamba日期:06/20/2018,浏览量:4959,Q&A: 0
    [Abstract] Retinal degeneration leads to loss of light-sensing photoreceptors eventually resulting in vision impairment and impose a heavy burden on both patients and the society. Currently available treatment options are very limited and mainly palliative. Ever since the discovery of human pluripotent stem cell technologies, cell replacement therapy has ...
    FACS-based Isolation of Neural and Glioma Stem Cell Populations from Fresh Human Tissues Utilizing EGF Ligand
    基于FACS利用EGF配体从新鲜人组织中分离神经和胶质瘤干细胞群
    作者:Jessica Tome-Garcia, Fiona Doetsch and Nadejda M. Tsankova日期:12/20/2017,浏览量:9619,Q&A: 0
    [Abstract] Direct isolation of human neural and glioma stem cells from fresh tissues permits their biological study without prior culture and may capture novel aspects of their molecular phenotype in their native state. Recently, we demonstrated the ability to prospectively isolate stem cell populations from fresh human germinal matrix and glioblastoma ...
    Transplantation of Embryonic Cortical Tissue into Lesioned Adult Brain in Mice
    将胚胎皮质组织移植到病变的成年小鼠脑中
    作者:Cong Wang, Hao Gao and Shengxiang Zhang日期:06/20/2017,浏览量:6257,Q&A: 0
    [Abstract] Transplantation of embryonic cortical tissue for repairing the damaged brain has provided a potential therapy for brain injury and diseases. The grafted tissue can successfully survive and participate in reestablishing the functional neural circuit of the host brain. Transplantation surgery can be combined with fluorescently labeled transgenic ...