生物化学

分类

    现刊
    Efficient and Rapid Analysis of Polysomes and Ribosomal Subunits in Cells and Tissues Using Ribo Mega-SEC
    利用Ribo Mega-SEC高效快速分析细胞和组织中的多聚体和核糖体亚基
    作者:Harunori Yoshikawa, Ramasubramanian Sundaramoorthy, Daniel Mariyappa, Hao Jiang and Angus I. Lamond日期:08/05/2021,浏览量:1214,Q&A: 0
    [Abstract]

    Polysome profile analysis is a popular method for separating polysomes and ribosomal subunits and is typically achieved using a sucrose density gradient (SDG). This has remained the gold standard method since ribosomes were first discovered; however, this method is time-consuming and requires multiple steps from making the gradient and long

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    A Detailed Protocol for Preparing Millimeter-sized Supergiant Liposomes that Permit Efficient Eukaryotic Cell-free Translation in the Interior
    一种制备毫米大小的允许在内部有效的真核细胞游离翻译的超巨大脂质体的详细方案
    作者:Hajime Takahashi and Atsushi Ogawa日期:06/20/2021,浏览量:880,Q&A: 0
    [Abstract]

    Liposomes have been used as a pseudo cell membrane for encapsulating biomolecules and creating an artificial cell in the interior where biochemical reactions can occur. Among the several methods used to prepare biomolecule-encapsulating liposomes, the spontaneous emulsion transfer method is superior to others in that it allows us to readily

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    Spin Labeling of RNA Using “Click” Chemistry for Coarse-grained Structure Determination via Pulsed Electron-electron Double Resonance Spectroscopy
    利用脉冲电子-电子双共振光谱测定粗粒结构的“点击”化学的RNA自旋标记
    作者:Maria F. Vicino, Tobias Hett and Olav Schiemann日期:05/05/2021,浏览量:2375,Q&A: 0
    [Abstract]

    Understanding the function of oligonucleotides on a molecular level requires methods for studying their structure, conformational changes, and internal dynamics. Various biophysical methods exist to achieve this, including the whole toolbox of Electron Paramagnetic Resonance (EPR or ESR) spectroscopy. An EPR method widely used in this regard is

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    Monitoring Real-time Temperature Dynamics of a Short RNA Hairpin Using Förster Resonance Energy Transfer and Circular Dichroism
    利用荧光共振能量转移和圆二色性监测短发夹RNA的实时温度动态
    作者:Martin Balcerowicz, Marco Di Antonio and Betty Y. W. Chung日期:03/20/2021,浏览量:2372,Q&A: 0
    [Abstract]

    RNA secondary structures are highly dynamic and subject to prompt changes in response to the environment. Temperature in particular has a strong impact on RNA structural conformation, and temperature-sensitive RNA hairpin structures have been exploited by multiple organisms to modify the rate of translation in response to temperature changes.

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    Trypanosomatid, fluorescence-based in vitro U-insertion/U-deletion RNA-editing (FIDE)
    基于荧光的锥虫体体外尿嘧啶插入或删除RNA编辑(FIDE)
    作者:Wolf-Matthias Leeder, Elisabeth Kruse and H. Ulrich Göringer日期:03/05/2021,浏览量:1781,Q&A: 0
    [Abstract]

    Gene expression within the mitochondria of African trypanosomes and other protozoan organisms relies on a nucleotide-specific RNA-editing reaction. In the process exclusively uridine (U)-nucleotides are site-specifically inserted into and deleted from sequence-deficient primary transcripts to convert them into translatable mRNAs. The reaction is

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    Identification of Intrinsic RNA Binding Specificity of Purified Proteins by in vitro RNA Immunoprecipitation (vitRIP)
    体外RNA免疫沉淀(vitRIP)鉴定纯化蛋白的RNA结合特异性
    作者:Marisa Müller, Tamas Schauer and Peter B. Becker日期:03/05/2021,浏览量:1522,Q&A: 0
    [Abstract]

    RNA-protein interactions are often mediated by dedicated canonical RNA binding domains. However, interactions through non-canonical domains with unknown specificity are increasingly observed, raising the question how RNA targets are recognized. Knowledge of the intrinsic RNA binding specificity contributes to the understanding of target

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    Plant ARGONAUTE Protein Immunopurification for Pathogen Cross Kingdom Small RNA Analysis
    植物ARGONAUTE蛋白免疫纯化用于病原体跨界小RNA分析
    作者:Florian Dunker, Bernhard Lederer and Arne Weiberg日期:02/05/2021,浏览量:2100,Q&A: 0
    [Abstract]

    Over the last decade, it has been noticed that microbial pathogens and pests deliver small RNA (sRNA) effectors into their host plants to manipulate plant physiology and immunity for infection, known as cross kingdom RNA interference. In this process, fungal and oomycete parasite sRNAs hijack the plant ARGONAUTE (AGO)/RNA-induced silencing complex

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    Ribosome Purification from an α-proteobacterium and rRNA Analysis by Northern Blot
    α-变形杆菌核糖体的纯化及Northern印迹分析
    作者:Liang Yin and Caroline S. Harwood日期:12/05/2020,浏览量:1036,Q&A: 0
    [Abstract] Ribosomes are an integral part of cellular life. They are complex molecular machines consisting of multiple ribosomal proteins and RNAs. To study different aspects of ribosome composition, many methods have been developed over the decades. Here, we describe how to purify ribosomes from the α-proteobacterium Rhodopseudomonas palustris ...
    Fluorescent Polysome Profiling in Caenorhabditis elegans
    秀丽隐杆线虫荧光多聚体分析
    作者:Dan Shaffer and Jarod A Rollins日期:09/05/2020,浏览量:2666,Q&A: 0
    [Abstract] An important but often overlooked aspect of gene regulation occurs at the level of protein translation. Many genes are regulated not only by transcription but by their propensity to be recruited to actively translating ribosomes (polysomes). Polysome profiling allows for the separation of unbound 40S and 60S subunits, 80S monosomes, and actively ...
    RNA Stability Measurements Using RT-qPCR in Arabidopsis Seedlings
    RT-qPCR法测定拟南芥幼苗RNA稳定性
    作者:Tianran Jia and Brandon H. Le日期:07/20/2020,浏览量:2138,Q&A: 0
    [Abstract] Steady-state mRNA levels are determined by both the rates of transcription and degradation. Regulation of mRNA stability and/or degradation are key factors that can significantly affect mRNA levels and its biological functions. mRNA stability can be measured indirectly after transcription inhibition. This protocol described a rapid and sensitive ...