癌症生物学

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    现刊
    Measurement of Bone Metastatic Tumor Growth by a Tibial Tumorigenesis Assay
    用胫骨肿瘤发生法测定骨转移瘤的生长
    作者:Baotong Zhang, Xin Li, Wei-Ping Qian, Daqing Wu and Jin-Tang Dong日期:11/20/2021,浏览量:1076,Q&A: 0
    [Abstract]

    Bone metastasis is a frequent and lethal complication of many cancer types (i.e., prostate cancer, breast cancer, and multiple myeloma), and a cure for bone metastasis remains elusive. To recapitulate the process of bone metastasis and understand how cancer cells metastasize to bone, intracardiac injection and intracaudal arterial animal models

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    Measuring Real-time DNA/RNA Nuclease Activity through Fluorescence
    通过荧光测量实时 DNA/RNA 核酸酶活性
    作者:Paulina Wyrzykowska, Sally Rogers and Richard Chahwan日期:11/05/2021,浏览量:1269,Q&A: 0
    [Abstract]

    DNA and RNA nucleases are wide-ranging enzymes, taking part in broad cellular processes from DNA repair to immune response control. Growing interest in the mechanisms and activities of newly discovered nucleases inspired us to share the detailed protocol of our nuclease assay (Sheppard et al., 2019). This easy and inexpensive method can provide

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    Comprehensive Identification of Translatable Circular RNAs Using Polysome Profiling
    利用多聚体谱综合鉴定可翻译环状RNA
    作者:Yanwen Ye, Zefeng Wang and Yun Yang日期:09/20/2021,浏览量:1200,Q&A: 0
    [Abstract]

    Circular RNAs (circRNAs), a special type of RNAs without 5’- and 3’-ends, are widely present in eukaryotes and known to function as noncoding RNAs to regulate gene expression, including as miRNA sponges. Recent studies showed that many exonic circRNAs, generated by back-splicing of pre-mRNAs, can be translated in a cap-independent

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    A Genetically Engineered Mouse Model of Venous Anomaly and Retinal Angioma-like Vascular Malformation
    静脉畸形和视网膜血管瘤样血管畸形的基因工程小鼠模型
    作者:Xudong Cao, Beibei Xu, Xiao Li, Taotao Li and Yulong He日期:08/05/2021,浏览量:1243,Q&A: 0
    [Abstract]

    Characterization of key regulators in vein development will advance our understanding of mechanisms underlying venous anomalies and provide therapeutic targets for the treatment of vascular malformations. Here, we provide a detailed protocol for the generation of genetically engineered mouse models targeting the Tek gene for the analysis of vein

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    Antisense Oligo Pulldown of Circular RNA for Downstream Analysis
    用于下游分析的环状RNA的反义Oligo Pulldown
    作者:Debojyoti Das, Aniruddha Das and Amaresh C. Panda日期:07/20/2021,浏览量:1320,Q&A: 0
    [Abstract]

    Circular RNAs (circRNAs) are a large family of noncoding RNA molecules that have emerged as novel regulators of gene expression by sequestering microRNAs (miRNAs) and RNA-binding proteins (RBPs). Several computational tools have been developed to predict circRNA interaction with target miRNAs and RBPs with a view to studying their potential effect

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    Intravenous and Non-invasive Drug Delivery to the Mouse Basal Forebrain Using MRI-guided Focused Ultrasound
    MRI引导下聚焦超声对小鼠基底前脑静脉及无创给药影响的研究
    作者:Kristiana Xhima, Dallan McMahon, Edward Ntiri, Maged Goubran, Kullervo Hynynen and Isabelle Aubert日期:06/20/2021,浏览量:1925,Q&A: 1
    [Abstract]

    Basal forebrain cholinergic neurons (BFCNs) regulate circuit dynamics underlying cognitive processing, including attention, memory, and cognitive flexibility. In Alzheimer’s disease and related neurodegenerative conditions, the degeneration of BFCNs has long been considered a key player in cognitive decline. The cholinergic system thus

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    Surface Engineering and Multimodal Imaging of Multistage Delivery Vectors in Metastatic Breast Cancer
    转移性乳腺癌多期转运载体的表面工程和多模态成像
    作者:Shreya Goel, Mauro Ferrari and Haifa Shen日期:05/20/2021,浏览量:3867,Q&A: 0
    [Abstract]

    The design of effective nanoformulations that target metastatic breast cancers is challenging due to a lack of competent imaging and image analysis protocols that can capture the interactions between the injected nanoparticles and metastatic lesions. Here, we describe the integration of in vivo whole-body PET-CT with high temporal resolution, ex

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    ATAC-Seq-based Identification of Extrachromosomal Circular DNA in Mammalian Cells and Its Validation Using Inverse PCR and FISH
    基于ATAC-Seq的哺乳动物细胞染色体外环状DNA鉴定及其反向PCR和FISH验证
    作者:Zhangli Su, Shekhar Saha, Teressa Paulsen, Pankaj Kumar and Anindya Dutta日期:05/05/2021,浏览量:4377,Q&A: 2
    [Abstract]

    Recent studies from multiple labs including ours have demonstrated the importance of extrachromosomal circular DNA (eccDNA) from yeast to humans (Shibata et al., 2012; Dillon et al., 2015; Møller et al., 2016; Kumar et al., 2017; Turner et al., 2017; Kim et al., 2020). More recently, it has been found that cancer cells obtain a selective

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    A Robust Mammary Organoid System to Model Lactation and Involution-like Processes
    一个健康的乳腺有机样系统来模拟哺乳和退化样过程
    作者:Elsa Charifou, Jakub Sumbal, Zuzana Koledova, Han Li and Aurélie Chiche日期:04/20/2021,浏览量:3212,Q&A: 0
    [Abstract]

    The mammary gland is a highly dynamic tissue that changes throughout reproductive life, including growth during puberty and repetitive cycles of pregnancy and involution. Mammary gland tumors represent the most common cancer diagnosed in women worldwide. Studying the regulatory mechanisms of mammary gland development is essential for understanding

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    Preparation of an Orthotopic, Syngeneic Model of Lung Adenocarcinoma and the Testing of the Antitumor Efficacy of Poly(2-oxazoline) Formulation of Chemo-and Immunotherapeutic Agents
    肺腺癌原位同源模型的制备及聚恶唑啉化疗和免疫治疗药物抗肿瘤疗效的测定
    [Abstract]

    Tumor xenograft models developed by transplanting human tissues or cells into immune-deficient mice are widely used to study human cancer response to drug candidates. However, immune-deficient mice are unfit for investigating the effect of immunotherapeutic agents on the host immune response to cancer (Morgan, 2012). Here, we describe the

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