生物化学

分类

    现刊
    Simple Methods for the Preparation of Colloidal Chitin, Cell Free Supernatant and Estimation of Laminarinase
    胶体几丁质、无细胞上清液的简易制备方法及昆布多糖酶的测定
    作者:Ananthamurthy Koteshwara日期:10/05/2021,浏览量:456,Q&A: 0
    [Abstract]

    Colloidal chitin (CC) is a common substrate used in research work involving chitin-active enzymes (chitinases). Cell free supernatant (CFS) is prepared from fermented broth. Preparation of CC and CFS usually involve large amounts of liquid, which must be separated from the solids. This necessitates the use of a large volume centrifugation

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    Analysis of Soluble Sugar Content in Minute Quantities of Rice Tissues by GC-MS
    气质联用技术分析水稻组织中微量可溶性糖含量
    作者:Li-he Xu, Li-ying Xiao, Xue-qiong Xiao, Yan-nong Xiao and Gao-feng Wang日期:07/05/2021,浏览量:1599,Q&A: 0
    [Abstract]

    Soluble sugars play key roles in plant growth, development, and adaption to the environment. Characterizing sugar content profiling of plant tissues promotes our understanding of the mechanisms underlying these plant processes. Several technologies have been developed to quantitate soluble sugar content in plant tissues; however, it is difficult

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    Chromatographic Assays for the Enzymatic Degradation of Chitin
    几丁质酶解的色谱分析
    作者:Sophanit Mekasha, Tina R. Tuveng, Gustav Vaaje-Kolstad and Vincent G. H. Eijsink日期:05/05/2021,浏览量:2075,Q&A: 0
    [Abstract]

    Chitin is an insoluble linear polymer of β(1→4)-linked N-acetylglucosamine. Enzymatic cleavage of chitin chains can be achieved using hydrolytic enzymes, called chitinases, and/or oxidative enzymes, called lytic polysaccharide monooxygenases (LPMOs). These two groups of enzymes have different modes of action and yield different product

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    Quantitative Characterization of the Amount and Length of (1,3)-β-D-glucan for Functional and Mechanistic Analysis of Fungal (1,3)-β-D-glucan Synthase
    通过(1,3)-β-d-葡聚糖量和长度的定量表征对真菌(1,3)-β-d-葡聚糖合酶进行功能和机理分析
    作者:Abhishek Chhetri, Anna Loksztejn and Kenichi Yokoyama日期:04/20/2021,浏览量:2151,Q&A: 0
    [Abstract]

    (1,3)-β-d-Glucan synthase (GS) is an essential enzyme for fungal cell wall biosynthesis that catalyzes the synthesis of (1,3)-β-d-glucan, a major and vital component of the cell wall. GS is a proven target of antifungal antibiotics including FDA-approved echinocandin derivatives; however, the function and mechanism of GS remain largely

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    Chromatographic Analysis for Targeted Metabolomics of Antioxidant and Flavor-Related Metabolites in Tomato
    番茄抗氧化剂和风味相关代谢产物靶向代谢组学的色谱分析
    作者:Nergiz Gürbüz Çolak, Neslihan Tek Eken, Anne Frary and Sami Doğanlar日期:03/05/2021,浏览量:2340,Q&A: 0
    [Abstract]

    Targeted metabolomics is a useful approach to evaluate crop breeding studies. Antioxidant and flavor-related traits are of increasing interest and are considered quality traits in tomato breeding. The present study presents chromatographic methods to study antioxidants (carotenoids, vitamin C, vitamin E, phenolic compounds, and glutathione) and

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    Bioorthogonal Labeling and Chemoselective Functionalization of Lung Extracellular Matrix
    肺细胞外基质的生物正交标记和化学选择性功能化
    作者:Zihan Ling, Yunhui Xing, Emily Reinoso Jacome, Shierly W. Fok and Xi Ren日期:02/20/2021,浏览量:1370,Q&A: 0
    [Abstract]

    Decellularized extracellular matrix (ECM) biomaterials derived from native tissues and organs are widely used for tissue engineering and wound repair. To boost their regenerative potential, ECM biomaterials can be functionalized via the immobilization of bioactive molecules. To enable ECM functionalization in a chemoselective manner, we have

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    Isolation of Tumor Cells Based on Their Distance from Blood Vessels
    基于与血管距离的肿瘤细胞分离
    作者:Saran Kumar, Husni Sharife, Tirzah Kreisel, Libat Bar-Lev, Myriam Grunewald and Eli Keshet日期:05/20/2020,浏览量:2769,Q&A: 0
    [Abstract] Differential exposure of tumor cells to microenvironmental cues greatly impacts cell phenotypes, raising a need for position based sorting of tumor cells amenable to multiple OMICs and functional analyses. One such key determinant of tumor heterogeneity in solid tumors is its vasculature. Proximity to blood vessels (BVs) profoundly affects tumor ...
    Negative Ion Mode nanoLC-ESI-MS/MS Analyses of Permethylated Sulfated Glycans
    甲基化硫酸多糖的负离子模式nanoLC-ESI-MS / MS分析
    作者:Shin-Yi Yu, Chu-Wen Cheng and Kay-Hooi Khoo日期:05/20/2020,浏览量:2152,Q&A: 0
    [Abstract] We have developed enabling techniques for sulfoglycomics based on MALDI-MS mapping and MS/MS sequencing of permethylated sulfated glycans. We then extended further the analytical workflow to C18 reverse phase (RP)-nanoLC-nanoESI-MS/MS analyses of permethylated sulfated glycans in the negative ion mode. The advantages are that extra sulfates on ...
    Permethylation and Microfractionation of Sulfated Glycans for MS Analysis
    硫酸多糖的全甲基化和微萃取质谱分析
    作者:Shin-Yi Yu, Sergei Snovida and Kay-Hooi Khoo日期:05/20/2020,浏览量:2166,Q&A: 0
    [Abstract] Sulfated glycans are barely detectable in routine mass spectrometry (MS)-based glycomic analysis due to ion suppression by the significantly more abundant neutral glycans in the positive ion mode, and sialylated non-sulfated glycans in the negative ion mode, respectively. Nevertheless, the negative charge imparted by sulfate can be advantageous ...
    Single-run HPLC Quantification of Plant Cell Wall Monosaccharides
    Single-run HPLC定量分析植物细胞壁单糖
    [Abstract] The plant cell wall is a complex network of polysaccharides and proteins that provides strength and structural integrity to plant cells, as well as playing a vital role in growth, development, and defense response. Cell wall polysaccharides can be broadly grouped into three categories: cellulose, pectins, and hemicelluloses. Dynamic interactions ...