发育生物学

分类

    现刊
    Live Imaging of Apoptotic Extrusion and Quantification of Apical Extrusion in Epithelial Cells
    上皮细胞凋亡挤压的活体成像和根尖挤压的定量分析
    作者:Kinga Duszyc, Ivar Noordstra, Alpha S. Yap and Guillermo A. Gomez日期:11/20/2021,浏览量:787,Q&A: 0
    [Abstract]

    Apoptotic cell death eliminates unhealthy cells and maintains homeostatic cell numbers within tissues. Epithelia, which serve as fundamental tissue barriers for the body, depend on a physical expulsion of dying cells (apoptotic cell extrusion) to remain sealed and intact. Apoptotic cell extrusion has been widely studied over recent years, with

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    Isolation of Primary Leydig Cells from Murine Testis
    从小鼠睾丸中分离出原发性睾丸间质细胞
    作者:Jinlian Liang, Yan Tang, Hanhao Li, Jiaxin Mei, Zhen Cao, Huan Xia, Rufei Huang, Yan Yang and Yadong Huang日期:11/20/2021,浏览量:317,Q&A: 0
    [Abstract]

    In males, Leydig cells are the primary source of testosterone, which is necessary for testis development, masculinization, and spermatogenesis. Leydig cells are a valuable cellular model for basic research; thus, it is important to develop an improved method for isolation and purification of Leydig cells from testes. The available methods for

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    Measurement of DNA Damage Using the Neutral Comet Assay in Cultured Cells
    在培养细胞中使用中性彗星试验测量 DNA 损伤
    作者:Elena Clementi, Zuzana Garajova and Enni Markkanen日期:11/20/2021,浏览量:1026,Q&A: 0
    [Abstract]

    Maintenance of DNA integrity is of pivotal importance for cells to circumvent detrimental processes that can ultimately lead to the development of various diseases. In the face of a plethora of endogenous and exogenous DNA damaging agents, cells have evolved a variety of DNA repair mechanisms that are responsible for safeguarding genetic

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    Wholemount in situ Hybridization for Spatial-temporal Visualization of Gene Expression in Early Post-implantation Mouse Embryos
    全座原位杂交技术对植入后早期小鼠胚胎中基因表达的时空可视化
    作者:Xianfa Yang, Yingying Chen, Lu Song, Ting Zhang and Naihe Jing日期:11/20/2021,浏览量:350,Q&A: 0
    [Abstract]

    Regionalized distribution of genes plays crucial roles in the formation of the spatial pattern in tissues and embryos during development. In situ hybridization has been one of the most widely used methods to screen, identify, and validate the spatial distribution of genes in tissues and embryos, due to its relative simplicity and low cost.

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    APEX-mediated Proximity Labeling of Proteins in Cells Targeted by Extracellular Vesicles
    APEX 介导的细胞外囊泡靶向细胞中蛋白质的邻近标记
    作者:Lu Song, Jun Chen, Angela Sun and Randy Schekman日期:11/05/2021,浏览量:962,Q&A: 0
    [Abstract]

    Extracellular vesicles (EVs) are thought to mediate intercellular communication through the delivery of cargo proteins and RNA to target cells. The uptake of EVs is often followed visually using lipophilic-dyes or fluorescently-tagged proteins to label membrane constituents that are then internalized into recipient cells (Christianson et al.,

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    Targeting the Expression of Long Noncoding RNAs in Murine Satellite Cells from Single Myofibers
    靶向小鼠卫星细胞中长链非编码 RNA 的表达
    作者:Martina Macino, Beatrice Biferali, Andrea Cipriano, Monica Ballarino and Chiara Mozzetta日期:11/05/2021,浏览量:853,Q&A: 0
    [Abstract]

    LncRNAs have been recently implicated in the epigenetic control of muscle differentiation and their functional characterization has traditionally relied upon in vitro models of myogenic differentiation. However, the use of experimental paradigms to specifically target lncRNAs expression in muscle stem cells (MuSCs), also known as satellite cells,

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    Intact in situ Preparation of the Drosophila melanogaster Lymph Gland for a Comprehensive Analysis of Larval Hematopoiesis
    黑腹果蝇淋巴腺的完整原位制备用于综合幼虫造血分析
    作者:Diana Rodrigues, K. VijayRaghavan, Lucas Waltzer and Maneesha S. Inamdar日期:11/05/2021,浏览量:1304,Q&A: 0
    [Abstract]

    Blood cells have a limited lifespan and are replenished by a small number of hematopoietic stem and progenitor cells (HSPCs). Adult vertebrate hematopoiesis occurs in the bone marrow, liver, and spleen, rendering a comprehensive analysis of the entire HSPC pool nearly impossible. The Drosophila blood system is well studied and has developmental,

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    Isolation and Culturing Primary Cholangiocytes from Mouse Liver
    小鼠肝原代胆管细胞的分离与培养
    [Abstract]

    Cholangiocytes are epithelial cells lining the intrahepatic and extrahepatic bile ducts. Cholangiocytes perform key physiological functions in the liver. Bile synthesized by hepatocytes is secreted into bile canaliculi, further stored in the gallbladder, and finally discharged into the duodenum. Due to liver injury, biliary epithelial proliferate

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    Structural and Functional Mapping of Mesenchymal Bodies
    间充质体的结构和功能图谱
    作者:Sébastien Sart, Raphaël F.-X. Tomasi, Antoine Barizien, Gabriel Amselem, Ana Cumano and Charles N. Baroud日期:10/05/2021,浏览量:1434,Q&A: 0
    [Abstract]

    The formation of spheroids with mesenchymal stem/stromal cells (MSCs), mesenchymal bodies (MBs), is usually performed using bioreactors or conventional well plates. While these methods promote the formation of a large number of spheroids, they provide limited control over their structure or over the regulation of their environment. It has

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    Phalloidin Staining of Actin Filaments for Visualization of Muscle Fibers in Caenorhabditis elegans
    秀丽隐杆线虫肌动蛋白丝的Phalloidin染色用于肌纤维可视化
    作者:Mario Romani and Johan Auwerx日期:10/05/2021,浏览量:1051,Q&A: 0
    [Abstract]

    Advances in C. elegans research have allowed scientists to recapitulate different human disorders, from neurodegenerative diseases to muscle dysfunction, in these nematodes. Concomitantly, the interest in visualizing organs affected by these conditions has grown, leading to the establishment of different antibody- and dye-based staining protocols

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