Intravenous and Non-invasive Drug Delivery to the Mouse Basal Forebrain Using MRI-guided Focused Ultrasound
    作者:Kristiana Xhima, Dallan McMahon, Edward Ntiri, Maged Goubran, Kullervo Hynynen and Isabelle Aubert日期:06/20/2021,浏览量:1451,Q&A: 1

    Basal forebrain cholinergic neurons (BFCNs) regulate circuit dynamics underlying cognitive processing, including attention, memory, and cognitive flexibility. In Alzheimer’s disease and related neurodegenerative conditions, the degeneration of BFCNs has long been considered a key player in cognitive decline. The cholinergic system thus

    Preparing Viable Hippocampal Slices from Adult Mice for the Study of Sharp Wave-ripples
    作者:Linhua Liu, Xiaojing Zhou and Jian-young Wu日期:10/05/2020,浏览量:1639,Q&A: 0
    [Abstract] We describe a protocol for preparing acute brain slices which can produce robust hippocampal sharp wave-ripples (SWRs) in vitro. The protocol is optimized for its simplicity and reliability for the preparation of solutions, slicing, and recovery incubation. Most slices in almost every mouse prepared though the protocol expressed vigorous ...
    Surgical Induction of Endometriosis in Female Mice
    作者:Alejandra Escudero-Lara, David Cabañero and Rafael Maldonado日期:09/20/2020,浏览量:1637,Q&A: 0
    [Abstract] Endometriosis is a common gynecological disease characterized by the presence of endometrial tissue outside the uterine cavity. It is frequently associated with pain, infertility and a reduced quality of life, and it lacks adequate treatment. Several rodent models of endometriosis have been developed through heterologous and homologous ...
    In vivo Blood-brain Barrier Permeability Assays Using Clostridium perfringens Epsilon Toxin
    作者:Michael R. Mazzucco, Timothy Vartanian and Jennifer R. Linden日期:08/05/2020,浏览量:2872,Q&A: 0
    [Abstract] In order for the brain to function properly, a carefully orchestrated homeostasis must be maintained. To help regulate this delicate balance, the brain has developed a highly selective blood-brain barrier (BBB). Under normal conditions, the BBB excludes harmful blood-borne material from the brain parenchyma. However, numerous neuropathological ...
    Inducing Alcohol Dependence in Rats Using Chronic Intermittent Exposure to Alcohol Vapor
    作者:Elizabeth M. Avegno and Nicholas W. Gilpin日期:05/05/2019,浏览量:3480,Q&A: 0
    [Abstract] Alcohol use disorder (AUD) is a significant public health and economic burden and is often characterized by repeated bouts of alcohol intoxication and withdrawal. Virtually all organ systems are impacted by chronic alcohol exposure. These effects can be investigated using the rat as a model organism; however, rats typically will not ...
    A New Efficient Method for Measuring Oxygen Consumption Rate Directly ex vivo in Human Epidermal Biopsies
    作者:Daniel Schniertshauer, Daniel Gebhard and Jörg Bergemann日期:03/05/2019,浏览量:4522,Q&A: 0
    [Abstract] Skin cells are constantly exposed to environmental influences such as air pollution, chemicals, pathogens and UV radiation. UV radiation can damage different biological structures, but most importantly cellular DNA. Mitochondria contain their own genome and accumulate UV-induced DNA mutations to a large extent. This can result, e.g., in ...
    Insulin Tolerance Test under Anaesthesia to Measure Tissue-specific Insulin-stimulated Glucose Disposal
    [Abstract] Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic ...
    Eye Drops for Delivery of Bioactive Compounds and BrdU to Stimulate Proliferation and Label Mitotically Active Cells in the Adult Rodent Retina
    作者:Cindy L. Linn, Sarah E. Webster and Mark K. Webster日期:11/05/2018,浏览量:3569,Q&A: 0
    [Abstract] Eye drop treatments are typically used to apply drugs to the anterior structures of the eye. Recently, however, studies have demonstrated that eye drops can reach the retina in the back of the eye if pharmacological agents are carried in appropriate vehicles. Here, we introduce an eye drop procedure to deliver a drug (PNU-282987), in combination ...
    Vascular Permeability Assay in Human Coronary and Mouse Brachiocephalic Arteries
    [Abstract] Coronary artery disease remains an important cause of morbidity and mortality. Previous work, including ours, has focused on the role of intraplaque hemorrhage, particularly from immature microvessel angiogenesis, as an important contributor to plaque progression via increases in vascular permeability leading to further intraplaque hemorrhage, ...
    Artificial Inhalation Protocol in Adult Mice
    作者:Thomas P. Eiting and Matt Wachowiak日期:09/20/2018,浏览量:3493,Q&A: 0
    [Abstract] Research in the area of in vivo olfactory physiology benefits from having direct access to the nasal airways through which odorants can be presented. Ordinarily, the passage of odorants through the airways is controlled by respiratory rhythm. This fact makes it difficult to control the timing and strength of an olfactory stimulus, since ...