Plasmodium cynomolgi Berok Growth Inhibition Assay by Thiol-reactive Probe Based Flow Cytometric Measurement
    作者:Jessica Jie Ying Ong, Bruce Russell and Jin-Hee Han日期:09/05/2021,浏览量:646,Q&A: 0

    The relapsing malaria species, Plasmodium vivax, is the most widely distributed and difficult-to-treat cause of human malaria. The merozoites of P. vivax preferentially invade ephemeral human CD71+ reticulocytes (nascent reticulocytes), thereby limiting the development of a robust continuous culture in vitro. Fortunately, P. vivax’s sister

    One-step White Blood Cell Extracellular Staining Method for Flow Cytometry
    作者:Ines Ait Belkacem, Pénélope Bourgoin, Jean Marc Busnel, Franck Galland and Fabrice Malergue日期:08/20/2021,浏览量:683,Q&A: 0

    Flow cytometry is a powerful analytical technique that is increasingly used in scientific investigations and healthcare; however, it requires time-consuming, multi-step sample procedures, which limits its use to specialized laboratories. In this study, we propose a new universal one-step method in which white blood cell staining and red blood cell

    Isolation of Microglia and Analysis of Protein Expression by Flow Cytometry: Avoiding the Pitfall of Microglia Background Autofluorescence
    作者:Jeremy C. Burns, Richard M. Ransohoff and Michaël Mingueneau日期:07/20/2021,浏览量:1488,Q&A: 0

    Microglia are a unique type of tissue-resident innate immune cell found within the brain, spinal cord, and retina. In the healthy nervous system, their main functions are to defend the tissue against infectious microbes, support neuronal networks through synapse remodeling, and clear extracellular debris and dying cells through phagocytosis. Many

    Sex-specific Separation of Plasmodium falciparum Gametocyte Populations
    作者:Melanie C. Ridgway, Daniela Cihalova and Alexander G. Maier日期:06/05/2021,浏览量:1275,Q&A: 0

    Plasmodium falciparum is a unicellular eukaryotic parasite that causes malaria in humans. The parasite is spread by Anopheles mosquitoes after ingestion of sexual stage parasites known as gametocytes. Malaria transmission depends on parasites switching from the disease-causing asexual blood forms to male and female gametocytes. The current

    FACS Enrichment of Total Interstitial Cells and Fibroblasts from Adult Mouse Ventricles
    作者:Nona Farbehi, Vaibhao Janbandhu, Robert E. Nordon and Richard P. Harvey日期:05/20/2021,浏览量:1481,Q&A: 0

    Besides cardiomyocytes, the heart contains numerous interstitial cell types, including cardiac fibroblasts, endothelial cells, immune (myeloid and lymphoid) cells, and mural cells (pericytes and vascular smooth muscle cells), which play key roles in heart repair, regeneration, and disease. We recently published a comprehensive map of cardiac

    Assay for Assessing Mucin Binding to Bacteria and Bacterial Proteins
    作者:Lubov S. Grigoryeva, Saima Rehman, Richard C. White, James A. Garnett and Nicholas P. Cianciotto日期:03/05/2021,浏览量:1572,Q&A: 0

    Legionella pneumophila, a Gram-negative bacterium and the causative agent of Legionnaires’ disease, exports over 300 effector proteins/virulence factors, through its type II (T2SS) and type IV secretion systems (T4SS). One such T2SS virulence factor, ChiA, not only functions as a chitinase, but also as a novel mucinase, which we believe aids

    A Simple Microplate Assay for Reactive Oxygen Species Generation and Rapid Cellular Protein Normalization
    作者:Neville S. Ng and Lezanne Ooi日期:01/05/2021,浏览量:2648,Q&A: 0
    [Abstract] 2',7'-dichlorofluorescein (DCF) and derivatives are commonly used as fluorescent indicators of a broad spectrum of reactive oxygen species (ROS) generation in cell-based assays. However, there are numerous challenges inherent to the utilization of DCF probes for intracellular microscopic analysis, including bleaching and probe efflux. Plate ...
    RNA ImmunoGenic Assay: A Method to Detect Immunogenicity of in vitro Transcribed mRNA in Human Whole Blood
    RNA ImmunoGenic分析:一种检测人全血体外转录mRNA免疫原性的方法
    [Abstract] The mRNA therapeutics is a new class of medicine to treat many various diseases. However, in vitro transcribed (IVT) mRNA triggers immune responses due to recognition by human endosomal and cytoplasmic RNA sensors, but incorporation of modified nucleosides have been shown to reduce such responses. Therefore, an assay signifying important ...
    Quantitative Kinetic Analyses of Histone Turnover Using Imaging and Flow Cytometry
    作者:Hanae Sato, Robert H. Singer and John M. Greally日期:09/05/2020,浏览量:2050,Q&A: 0
    [Abstract] Dynamic histone changes occur as a central part of chromatin regulation. Deposition of histone variants and post-translational modifications of histones are strongly associated with properties of chromatin status. Characterizing the kinetics of histone variants allows important insights into transcription regulation, chromatin maintenance and ...
    Multiplication and Growth Inhibition Activity Assays for the Zoonotic Malaria Parasite, Plasmodium knowlesi
    作者:Franziska Mohring, Thomas A. Rawlinson, Simon J. Draper and Robert W. Moon日期:09/05/2020,浏览量:2656,Q&A: 0
    [Abstract] Malaria remains a major cause of morbidity and mortality globally. Clinical symptoms of the disease arise from the growth and multiplication of Plasmodium parasites within the blood of the host. Thus in vitro assays to determine how drug, antibody and genetic perturbations affect the growth rate of Plasmodium parasites ...