发育生物学

分类

    现刊
    Visualization and Quantitation of Wg trafficking in the Drosophila Wing Imaginal Epithelium
    果蝇翅成虫上皮中Wg运输的可视化和定量研究
    作者:Leonie Witte, Karen Linnemannstöns, Mona Honemann-Capito and Julia Christina Gross日期:06/05/2021,浏览量:1398,Q&A: 0
    [Abstract]

    Secretory Wnt trafficking can be studied in the polarized epithelial monolayer of Drosophila wing imaginal discs (WID). In this tissue, Wg (Drosophila Wnt-I) is presented on the apical surface of its source cells before being internalized into the endosomal pathway. Long-range Wg secretion and spread depend on secondary secretion from endosomal

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    Optimized Immunostaining of Embryonic and Early Postnatal Mouse Brain Sections
    胚胎和产后早期小鼠脑切片的优化免疫染色
    作者:Kawssar Harb, Michele Bertacchi and Michèle Studer日期:01/05/2021,浏览量:1776,Q&A: 0
    [Abstract]

    The mammalian neocortex, the outer layer of the cerebrum and most recently evolved brain region, is characterized by its unique areal and laminar organization. Distinct cortical layers and areas can be identified by the protein expression of graded transcription factors and molecular determinants that define the identity of different projection

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    Multitarget Immunohistochemistry for Confocal and Super-resolution Imaging of Plant Cell Wall Polysaccharides
    植物细胞壁多糖共聚焦和超分辨成像的多靶点免疫组织化学研究
    作者:Kalina T. Haas, Methieu Rivière, Raymond Wightman and Alexis Peaucelle日期:10/05/2020,浏览量:2771,Q&A: 0
    [Abstract] The plant cell wall (PCW) is a pecto-cellulosic extracellular matrix that envelopes the plant cell. By integrating extra-and intra-cellular cues, PCW mediates a plethora of essential physiological functions. Notably, it permits controlled and oriented tissue growth by tuning its local mechano-chemical properties. To refine our knowledge of these ...
    Application of Mechanical Forces on Drosophila Embryos by Manipulation of Microinjected Magnetic Particles
    机械力应用于果蝇胚胎的磁性粒子显微注射操作
    作者:Arturo D’Angelo and Jérôme Solon日期:05/05/2020,浏览量:1866,Q&A: 0
    [Abstract] Cells generate mechanical forces to shape tissues during morphogenesis. These forces can activate several biochemical pathways and trigger diverse cellular responses by mechano-sensation, such as differentiation, division, migration and apoptosis. Assessing the mechano-responses of cells in living organisms requires tools to apply controlled local ...
    Live Cell Imaging of Male Meiosis in Arabidopsis by a Landmark-based System
    地标系统在拟南芥雄性减数分裂活体细胞成像中的应用
    作者:Maria Ada Prusicki, Emma Mathilde Keizer, Rik Peter van Rosmalen, Christian Fleck and Arp Schnittger日期:05/05/2020,浏览量:3306,Q&A: 0
    [Abstract] Live cell imaging has tremendously promoted our understanding of cellular and subcellular processes such as cell division. Here, we present a step-by-step protocol for a robust and easy-to-use live cell imaging approach to study male meiosis in the plant Arabidopsis thaliana as recently established. Our method relies on the concomitant ...
    Bimolecular Fluorescence Complementation (BiFC) for Studying Sarcomeric Protein Interactions in Drosophila
    双分子荧光互补法研究果蝇肌节蛋白相互作用
    作者:Océane Marescal, Frieder Schöck and Nicanor González-Morales日期:04/05/2020,浏览量:2877,Q&A: 0
    [Abstract] Protein-protein interactions in Drosophila myofibrils are essential for their function and formation. Bimolecular Fluorescence Complementation (BiFC) is an effective method for studying protein interactions and localization. BiFC relies on the reconstitution of a monomeric fluorescent protein from two half-fragments when in proximity. Two ...
    Electron Microscopic Detection of Proteins and Protein Complexes in Mammalian Cells Using APEX-tagged, Conditionally Stable Nanobodies
    利用APEX标记的条件性稳定纳米体电子显微检测哺乳动物细胞中的蛋白和蛋白复合物
    作者:Thomas E. Hall, James Rae, Nicholas Ariotti and Robert G. Parton日期:11/20/2018,浏览量:3752,Q&A: 0
    [Abstract] The recent development of 3D electron microscopic techniques for cells and tissues has necessitated the development of new methods for the detection of proteins and protein-complexes in situ. The development of new genetic tags, such as the ascorbate peroxidase, APEX, for electron microscopic detection of tagged proteins has expanded the ...
    Transmission Electron Microscopy for Analysis of Mitochondria in Mouse Skeletal Muscle
    使用透射电子显微镜技术分析小鼠骨骼肌线粒体
    作者:Joseph D. McMillan and Michael A. Eisenback日期:05/20/2018,浏览量:13449,Q&A: 0
    [Abstract] Skeletal muscle is the most abundant tissue in the human body and regulates a variety of functions including locomotion and whole-body metabolism. Skeletal muscle has a plethora of mitochondria, the organelles that are essential for aerobic generation of ATP which provides the chemical energy to fuel vital functions such as contraction. The number ...
    Construction and Cloning of Minigenes for in vivo Analysis of Potential Splice Mutations
    用于体内潜在的剪接突变分析的微小基因的构建及克隆
    作者:Lisa Maria Riedmayr, Sybille Böhm, Stylianos Michalakis and Elvir Becirovic日期:03/05/2018,浏览量:8982,Q&A: 0
    [Abstract] Disease-associated mutations influencing mRNA splicing are referred to as splice mutations. The majority of splice mutations are found on exon-intron boundaries defining canonical donor and acceptor splice sites. However, mutations in the coding region (exonic mutations) can also affect mRNA splicing. Exact knowledge of the disease mechanism of ...
    Preparation of Precisely Oriented Cryosections of Undistorted Drosophila Wing Imaginal Discs for High Resolution Confocal Imaging
    制备未畸变果蝇翅成虫盘精确定向冷冻切片以用于高分辨率共焦成像
    作者:Samuel Petshow and Marcel Wehrli日期:02/05/2018,浏览量:6919,Q&A: 0
    [Abstract] The combination of immunofluorescence and laser scanning confocal microscopy (LSM) is essential to high-resolution detection of molecular distribution in biological specimens. A frequent limitation is the need to image deep inside a tissue or in a specific plane, which may be inaccessible due to tissue size or shape. Recreating high-resolution 3D ...