细胞生物学

分类

    现刊
    A Novel Method to Make Polyacrylamide Gels with Mechanical Properties Resembling those of Biological Tissues
    一种制备具有类似生物组织力学性能的聚丙烯酰胺凝胶的新方法  
    作者:Katarzyna Pogoda, Elisabeth E. Charrier and Paul A. Janmey日期:08/20/2021,浏览量:949,Q&A: 0
    [Abstract]

    Studies characterizing how cells respond to the mechanical properties of their environment have been enabled by the use of soft elastomers and hydrogels as substrates for cell culture. A limitation of most such substrates is that, although their elastic properties can be accurately controlled, their viscous properties cannot, and cells

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    A Transient Transfection-based Cell Adhesion Assay with 293T Cells
    基于瞬时转染的293T细胞粘附实验
    作者:Rohit Singh and Beom K. Choi日期:01/05/2021,浏览量:2469,Q&A: 0
    [Abstract]

    The in vitro cell adhesion assay is a quantitative method for measuring selective cell adhesion to specific proteins. Traditionally, cell adhesion assays employ purified protein immobilized on a solid glass or plastic surface. Here, we describe a transient 293T cell transfection-based cell adhesion assay to study selective cell adhesion of a

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    An in vitro DNA Sensor-based Assay to Measure Receptor-specific Adhesion Forces of Eukaryotic Cells and Pathogens
    基于DNA传感器的真核细胞与病原体受体特异性粘附力的体外检测
    作者:Maurizio Wack, Tina Wiegand, Friedrich Frischknecht and E. Ada Cavalcanti-Adam日期:09/05/2020,浏览量:2080,Q&A: 0
    [Abstract] Motility of eukaryotic cells or pathogens within tissues is mediated by the turnover of specific interactions with other cells or with the extracellular matrix. Biophysical characterization of these ligand-receptor adhesions helps to unravel the molecular mechanisms driving migration. Traction force microscopy or optical tweezers are typically ...
    Adhesion of Enteroaggregative E. coli Strains to HEK293 Cells
    肠凝聚型大肠埃希杆菌对HEK293细胞的粘附测定
    作者:Jorge Luis Ayala-Lujan and Fernando Ruiz-Perez日期:04/20/2018,浏览量:4686,Q&A: 0
    [Abstract] Enteroaggregative Escherichia coli (EAEC) is a recognized cause of acute diarrhea among both children and adults worldwide. EAEC strains are characterized by the presence of aggregative adherence fimbriae (AAF), which play a key role in pathogenesis by mediating attachment to the intestinal mucosa and by triggering host inflammatory ...
    Measurement of Mesenchymal Stem Cells Attachment to Endothelial Cells
    间充质干细胞附着于内皮细胞的测定
    作者:Shan Wang, Chris D. Madsen and Yaojiong Wu日期:03/20/2018,浏览量:5496,Q&A: 1
    [Abstract] Mesenchymal stem cells (MSCs) have shown profound therapeutic potential in tissue repair and regeneration. However, recent studies indicate that MSCs are largely entrapped in lungs after intravenous delivery and die shortly. The underlying mechanisms have been poorly understood. We have provided evidence to show that excess expression and ...
    Quantification of Neisseria meningitidis Adherence to Human Epithelial Cells by Colony Counting
    通过菌落计数定量脑膜炎奈瑟菌对人上皮细胞的粘附能力
    作者:Sara Sigurlásdóttir, Sunil D Saroj, Olaspers Sara Eriksson, Jens Eriksson and Ann-Beth Jonsson日期:02/05/2018,浏览量:6034,Q&A: 0
    [Abstract] To cause an infection, the human specific pathogen Neisseria meningitides must first colonize the nasopharynx. Upon tight interaction with the mucosal epithelium, N. meningitidis may cross the epithelial cellular barrier, reach the bloodstream and cause sepsis and/or meningitis. Since N. meningitidis niche is restricted ...
    Using xCELLigence RTCA Instrument to Measure Cell Adhesion
    使用xCELLigence RTCA仪器测量细胞粘附
    作者:Hellyeh Hamidi, Johanna Lilja and Johanna Ivaska日期:12/20/2017,浏览量:9763,Q&A: 0
    [Abstract] Cell adhesion to neighbouring cells and to the underlying extracellular matrix (ECM) is a fundamental requirement for the existence of multicellular organisms. As such, the formation, stability and dissociation of cell adhesions are subject to tight control in space and time and perturbations within the sophisticated adhesion machinery are ...
    Adhesion and Invasion Assay Procedure Using Caco-2 Cells for Listeria monocytogenes
    单核细胞增生李斯特菌对Caco-2细胞的粘附和侵袭试验程序
    作者:Swetha Reddy and Frank Austin日期:05/05/2017,浏览量:12057,Q&A: 0
    [Abstract] Listeria monocytogenes is an important Gram-positive foodborne pathogen that is a particular problem in ready-to-eat food. It has an ability to survive in harsh conditions like refrigeration temperatures and high salt concentrations and is known to cross intestinal, placental and blood-brain barriers. Several cancerous cell lines like ...