细胞生物学

分类

    现刊
    Single Cell Analysis and Sorting of Aspergillus fumigatus by Flow Cytometry
    用流式细胞仪对烟曲霉进行单细胞分析和分选
    作者:Gareth Howell and Robert-Jan Bleichrodt日期:04/20/2021,浏览量:1670,Q&A: 0
    [Abstract]

    Experimental results in fungal biology research are usually obtained as average measurements across whole populations of cells, whilst ignoring what is happening at the single cell level. Microscopy has allowed us to study single-cell behavior, but it has low throughput and cannot be used to select individual cells for downstream experiments. Here

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    A Flow Cytometric Method to Determine Transfection Efficiency
    利用流式细胞术测定转染效率
    [Abstract] Mammalian cell transfection is a powerful technique commonly used in molecular biology to express exogenous DNA or RNA in cells and study gene and protein function. Although several transfection strategies have been developed, there is a wide variation with regards to transfection efficiency, cell toxicity and reproducibility. Thus, a sensitive ...
    High Dimensional Functionomic Analysis of Human Hematopoietic Stem and Progenitor Cells at a Single Cell Level
    人造血干细胞和祖细胞在单细胞水平上的高维功能组学分析
    作者:Thomas Luh, Kimberly Lucero, Wenji Ma, Jaeyop Lee, Yu Jerry Zhou, Yufeng Shen and Kang Liu日期:05/20/2018,浏览量:5215,Q&A: 0
    [Abstract] The ability to conduct investigation of cellular transcription, signaling, and function at the single-cell level has opened opportunities to examine heterogeneous populations at unprecedented resolutions. Although methods have been developed to evaluate high-dimensional transcriptomic and proteomic data (relating to cellular mRNA and protein), ...
    Flow Cytometric Analysis of HIV-1 Transcriptional Activity in Response to shRNA Knockdown in A2 and A72 J-Lat Cell Lines
    A2和A72 J-Lat细胞系中HIV-1经shRNA沉默后转录活性的流式细胞分析
    作者:Daniela Boehm and Melanie Ott日期:06/05/2017,浏览量:7186,Q&A: 0
    [Abstract] The main obstacle to eradicating HIV-1 from patients is post-integration latency (Finzi et al., 1999). Antiretroviral treatments target only actively replicating virus, while latent infections that have low or no transcriptional activity remain untreated (Sedaghat et al., 2007). To eliminate viral reservoirs, one strategy focuses ...
    Flow Cytometric Analysis of Drug-induced HIV-1 Transcriptional Activity in A2 and A72 J-Lat Cell Lines
    A2和A72 J-Lat细胞系中药物诱导的HIV-1转录活性的流式细胞术分析
    作者:Daniela Boehm and Melanie Ott日期:05/20/2017,浏览量:8795,Q&A: 0
    [Abstract] The main obstacle to eradicating HIV-1 from patients is post-integration latency (Finzi et al., 1999). Antiretroviral treatments target only actively replicating virus, while latent infections that have low or no transcriptional activity remain untreated (Sedaghat et al., 2007). A combination of antiretroviral treatments with ...