神经科学

分类

    现刊
    Generation of Human iPSC-derived Neural Progenitor Cells (NPCs) as Drug Discovery Model for Neurological and Mitochondrial Disorders
    人来源iPSC获得的神经祖细胞(NPCs)的产生作为神经和线粒体疾病的药物发现模型
    作者:Annika Zink, Pawel Lisowski and Alessandro Prigione日期:03/05/2021,浏览量:2524,Q&A: 1
    [Abstract]

    The high attrition rate in drug development processes calls for additional human-based model systems. However, in the context of brain disorders, sampling live neuronal cells for compound testing is not applicable. The use of human induced pluripotent stem cells (iPSCs) has revolutionized the field of neuronal disease modeling and drug discovery.

    ...
    Co-culture of Murine Neurons Using a Microfluidic Device for The Study of Tau Misfolding Propagation
    利用微流体装置共培养小鼠神经元研究Tau的错误折叠传播
    作者:Grace I Hallinan, Dianne M Lopez, Mariana Vargas-Caballero, Jonathan West and Katrin Deinhardt日期:08/20/2020,浏览量:2084,Q&A: 0
    [Abstract] The deposition of misfolded, aggregated tau protein is a hallmark of several neurodegenerative diseases, collectively termed “tauopathies”. Tau pathology spreads throughout the brain along connected pathways in a prion-like manner. The process of tau pathology propagation across circuits is a focus of intense research and has been investigated in ...
    Calcein Release Assay to Measure Membrane Permeabilization by Recombinant Alpha-Synuclein
    钙黄绿素释放法测定重组α-突触核蛋白膜通透性
    作者:Sayan Dutta, Ben G. Watson, Seema Mattoo and Jean-Christophe Rochet日期:07/20/2020,浏览量:2847,Q&A: 0
    [Abstract] Lipid membranes are involved in regulating biochemical and biological processes and in modulating the selective permeability of cells, organelles, and vesicles. Membrane composition, charge, curvature, and fluidity all have concerted effects on cellular signaling and homeostasis. The ability to prepare artificial lipid assemblies that mimic ...
    An ex vivo Approach to Assess Mitochondrial ROS by Flow Cytometry in AAV-tagged Astrocytes in Adult Mice
    一种利用流式细胞术进行成年大鼠腺相关病毒标记星型胶质细胞中线粒体ROS水平的体外检测方法
    作者:Carlos Vicente-Gutierrez and Juan P. Bolaños日期:03/20/2020,浏览量:2787,Q&A: 0
    [Abstract] Mitochondrial reactive oxygen species (mROS) are naturally produced signalling molecules extremely relevant for understanding both health- and disease-associated biological processes. The study of mROS in the brain is currently underway to decipher their physiopathological roles and contributions in neurological diseases. Recent advances in this ...
    Hippocampal Unicellular Recordings and Hippocampal-dependent Innate Behaviors in an Adolescent Mouse Model of Alzheimer’s disease
    阿尔茨海默症幼鼠模型中海马单细胞记录和海马依赖先天性行为
    [Abstract] Transgenic mice have been used to make valuable contributions to the field of neuroscience and model neurological diseases. The simultaneous functional analysis of hippocampal cell activity combined with hippocampal dependent innate task evaluations provides a reliable experimental approach to detect fine changes during early phases of ...
    Mitochondrial Respiratory Measurements in Patient-derived Fibroblasts
    患者来源成纤维细胞中的线粒体呼吸测定
    作者:Prashant Mishra, Ting Zhang, Ming Guo and David Chan日期:12/05/2019,浏览量:2902,Q&A: 0
    [Abstract] Mitochondrial dysfunction is associated with a number of human diseases. As an example, we recently established in vivo Drosophila models of IBMPFD (Inclusion body myopathy, Paget disease, and frontotemporal dementia), and uncovered that human disease mutations of the p97/VCP (Valosin Containing Protein) gene behave as ...
    Purification of Soluble Recombinant Human Tau Protein from Bacteria Using Double-tag Affinity Purification
    双标签亲和纯化法从细菌中纯化人可溶重组Tau蛋白
    作者:Joseph McInnes, Lujia Zhou and Patrik Verstreken日期:11/20/2018,浏览量:5504,Q&A: 1
    [Abstract] Dysfunction of the microtubule-associated protein Tau (encoded by the MAPT gene) has been implicated in more than twenty neurodegenerative diseases, including Alzheimer’s. As such, the physiological and disease-relevant functions of Tau have garnered great interest in the research community. One barrier hampering investigations into the ...
    Ex vivo Whole-cell Recordings in Adult Drosophila Brain
    成年果蝇脑体外全细胞记录
    作者:Alexa J. Roemmich, Soleil S. Schutte and Diane K. O’Dowd日期:07/20/2018,浏览量:5050,Q&A: 0
    [Abstract] Cost-effective and efficient, the fruit fly (Drosophila melanogaster) has been used to make many key discoveries in the field of neuroscience and to model a number of neurological disorders. Great strides in understanding have been made using sophisticated molecular genetic tools and behavioral assays. Functional analysis of neural ...
    Intracellular and Mitochondrial Reactive Oxygen Species Measurement in Primary Cultured Neurons
    原代培养的神经元细胞和线粒体中活性氧的测定
    [Abstract] Reactive oxygen species (ROS) are chemically reactive oxygen containing molecules. ROS consist of radical oxygen species including superoxide anion (O2•−) and hydroxyl radical (•OH) and non-radical oxygen species such as hydrogen peroxide (H2O2), singlet oxygen (O2). ROS are generated by ...
    Sacral Spinal Cord Transection and Isolated Sacral Cord Preparation to Study Chronic Spinal Cord Injury in Adult Mice
    骶髓横断后制备骶髓分离物来研究成年小鼠慢性脊髓损伤
    作者:Carmelo Bellardita, Maite Marcantoni, Peter Löw and Ole Kiehn日期:04/05/2018,浏览量:5382,Q&A: 0
    [Abstract] Spinal cord injury (SCI) is characterized by multiple sensory/motor impairments that arise from different underlying neural mechanisms. Linking specific sensory/motor impairments to neural mechanism is limited by a lack of direct experimental access to these neural circuits. Here, we describe an experimental model which addresses this shortcoming. ...