细胞生物学

分类

    现刊
    Characterization of Biological Motion Using Motion Sensing Superpixels
    基于运动传感超像素的生物运动特性研究
    作者:Felix Y. Zhou, Carlos Ruiz-Puig, Richard P. Owen, Michael J. White, Jens Rittscher and Xin Lu日期:09/20/2019,浏览量:707,Q&A: 0
    [Abstract] Precise spatiotemporal regulation is the foundation for the healthy development and maintenance of living organisms. All cells must correctly execute their function in the right place at the right time. Cellular motion is thus an important dynamic readout of signaling in key disease-relevant molecular pathways. However despite the rapid ...
    A Novel Technique for Imaging and Analysis of Hair Cells in the Organ of Corti Using Modified Sca/eS and Machine Learning
    利用改进的Sca/eS和机器学习用于螺旋器中毛细胞的成像和分析
    [Abstract] Here, we describe a sorbitol-based optical clearing method, called modified Sca/eS that can be used to image all hair cells (HCs) in the mouse cochlea. This modification of Sca/eS is defined by three steps: decalcification, de-lipidation, and refractive index matching, which can all be completed within 72 h. Furthermore, we established automated ...
    Looking through Brains with Fast Passive CLARITY: Zebrafish, Rodents, Non-human Primates and Humans
    利用 Fast Passive CLARITY技术研究斑马鱼、啮齿动物、灵长类动物和人类的大脑
    作者:Farzad Mortazavi, Alexander J. Stankiewicz and Irina V. Zhdanova日期:08/05/2019,浏览量:1576,Q&A: 0
    [Abstract] Recently developed CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ-hybridization-compatible Tis-sue-hYdrogel) technique renders the tissue transparent by removing lipids in the tissue, while preserving and stabilizing the cellular and subcellular structures. This provides effective penetration of diverse ...
    Immunofluorescence Staining of WT-1/Podocalyxin on Mouse Kidney Sections
    免疫荧光标记小鼠肾脏切片中的WT-1/足糖萼
    作者:Carole Henique, Olivia Lenoir and Pierre-Louis Tharaux日期:04/20/2019,浏览量:1335,Q&A: 0
    [Abstract] In glomerular diseases, podocytes are one type of cells involved in dysfunction of glomerular filtration. In these conditions, podocyte proteins expression change. Therefore, immunofluorescent staining of podocytes can be performed to visualize podocyte localization of proteins of interest. In this protocol, we detail a method to stain podocytes ...
    A Method for Culturing Mouse Whisker Follicles to Study Circadian Rhythms ex vivo
    一种离体研究昼夜节律的小鼠触须毛囊培养方法
    作者:Atsuhiro Nishida, Yoshiki Miyawaki, Koichi Node and Makoto Akashi日期:01/20/2019,浏览量:1547,Q&A: 0
    [Abstract] Ex vivo tissue-culture experiments are often performed in the field of circadian biology. The major aim of these experiments is to evaluate circadian characteristics such as period length at the tissue-autonomous level by monitoring clock gene expression in real time. This culture method is also used to examine the tissue specificity of ...
    An Image Analysis Pipeline to Quantify Emerging Cracks in Materials or Adhesion Defects in Living Tissues
    分析活体组织中出现的材料裂缝或附着力缺陷的图像分析法
    作者:Stéphane Verger, Guillaume Cerutti and Olivier Hamant日期:10/05/2018,浏览量:2333,Q&A: 0
    [Abstract] Microcracks in materials reflect their mechanical properties. The quantification of the number or orientation of such cracks is thus essential in many fields, including engineering and geology. In biology, cracks in soft tissues can reflect adhesion defects, and the analysis of their pattern can help to deduce the magnitude and orientation of ...
    A Method to Injure, Dissect and Image Indirect Flight Muscle of Drosophila
    果蝇间接飞行肌的损伤、解剖和成像方法
    作者:Kunal Chakraborty, K. VijayRaghavan and Rajesh Gunage日期:05/20/2018,浏览量:4083,Q&A: 0
    [Abstract] Inducing an injury specifically to Drosophila flight muscles is a difficult task, owing to the small size of the muscles and the presence of the cuticle. The protocol described below provides an easy and reproducible method to induce injury in the Drosophila flight muscles.
    Quantification of Bacterial Attachment to Tissue Sections
    组织切片细菌附着定量
    作者:Batya Isaacson, Tehila Hadad, Gilad Bachrach and Ofer Mandelboim日期:03/05/2018,浏览量:3850,Q&A: 0
    [Abstract] Here we describe a method to test bacterial adhesion to paraffin embedded tissue sections. This method allows examining binding of different bacterial strains, transfected with a fluorescent protein reporter plasmid to various tissues, to better understand different mechanisms such as colonization. This assay provides a more physiological context ...
    Obtaining Acute Brain Slices
    急性脑组织切片的制备
    作者:Thomas Papouin and Philip G. Haydon日期:01/20/2018,浏览量:8546,Q&A: 1
    [Abstract] Obtaining acute brain slices for electrophysiology or amperometric recordings has become a routine procedure in most labs in the field of neuroscience. Yet, protocols describing the step by step process are scarce, in particular for routine acute preparations such as from the mouse hippocampus. Here we provide a detailed protocol for the ...
    Micro-computed Tomography to Visualize Vascular Networks in Maize Stems
    玉米茎杆中维管网络的微型计算机断层摄影术观察
    作者:Akiteru Maeno and Katsutoshi Tsuda日期:01/05/2018,浏览量:4788,Q&A: 0
    [Abstract] Plant vascular systems in the stem connect roots with aerial organs to move solutes containing minerals, nutrients as well as signaling molecules, and therefore, they play pivotal roles in plant growth and development. However, stem vascular systems, especially in crop species, have been poorly described since they are deeply embedded in the ...