神经科学

分类

    现刊
    A Co-purification Method for Efficient Production and Src Kinase-mediated Phosphorylation of Aplysia Cortactin
    一种高效生产和Src激酶介导的马海兔磷酸化的共纯化方法
    作者:Sherlene L. Brown, Yuan Ren, Daniel M. Suter and Seema Mattoo日期:09/20/2021,浏览量:272,Q&A: 0
    [Abstract]

    Cortactin is an actin-binding protein that regulates processes like cell migration, endocytosis, and tumor cell metastasis. Although cortactin is associated with actin-cytoskeletal dynamics in non-neuronal cells and cell-free systems, the exact mechanisms underlying its fundamental roles in neuronal growth cones are not fully explored. Recent

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    In vitro AMPylation/Adenylylation of Alpha-synuclein by HYPE/FICD
    HYPE/FICD法体外氨酰化/腺苷酰化α-突触核蛋白
    作者:Ali Camara, Anwesha Sanyal, Sayan Dutta, Jean-Christophe Rochet and Seema Mattoo日期:09/20/2020,浏览量:2047,Q&A: 0
    [Abstract] One of the major histopathological hallmarks of Parkinson’s disease are Lewy bodies (LBs) –cytoplasmic inclusions, enriched with fibrillar forms of the presynaptic protein alpha-synuclein (α-syn). Progressive deposition of α-syn into LBs is enabled by its propensity to fibrillize into insoluble aggregates. We recently described a marked reduction ...
    Isolation and Imaging of His- and RFP-tagged Amyloid-like Proteins from Caenorhabditis elegans by TEM and SIM
    TEM和SIM技术用于秀丽隐杆线虫His-和RFP标记淀粉样蛋白的分离和成像
    作者:Amberley D. Stephens, Meng Lu and Gabriele S. Kaminski Schierle日期:11/05/2019,浏览量:2438,Q&A: 0
    [Abstract] In our recently published paper, we highlight that during normal aging of C. elegans age-dependent aggregates of proteins form and lead to functional decline of tissues. The protocol described here details the isolation of two proteins from C. elegans in their aggregated amyloid-like form, casein kinase I isoform alpha (KIN-19) ...
    Purification of Soluble Recombinant Human Tau Protein from Bacteria Using Double-tag Affinity Purification
    双标签亲和纯化法从细菌中纯化人可溶重组Tau蛋白
    作者:Joseph McInnes, Lujia Zhou and Patrik Verstreken日期:11/20/2018,浏览量:5513,Q&A: 1
    [Abstract] Dysfunction of the microtubule-associated protein Tau (encoded by the MAPT gene) has been implicated in more than twenty neurodegenerative diseases, including Alzheimer’s. As such, the physiological and disease-relevant functions of Tau have garnered great interest in the research community. One barrier hampering investigations into the ...
    Isolation of Chromatin-bound Proteins from Subcellular Fractions for Biochemical Analysis
    从亚细胞成分中分离用于生化分析的染色质结合蛋白
    作者:Sébastien Gillotin日期:10/05/2018,浏览量:13993,Q&A: 5
    [Abstract] Shuttling of proteins between different cellular compartments controls their proteostasis and can contribute in some cases to regulate their activity. Biochemical analysis of chromatin-bound proteins, such as transcription factors, is often difficult because of their low yield and due to the interference from nucleic acids. This protocol describes ...
    Preparation, Stimulation and Other Uses of Adult Rat Brain Synaptosomes
    成年大鼠脑突触体的制备、刺激及其他用途
    作者:Jigar Modi, Howard Prentice and Jang-Yen Wu日期:12/20/2017,浏览量:4346,Q&A: 0
    [Abstract] In this paper, our protocol for preparation of brain synaptosomes is described. Synaptosomes are a valuable model system for analysis of structural components of the synapse as well as for investigation of synaptic function. Synaptosomal preparations are necessary for understanding molecular changes at synapses where critical post-translational ...
    Extraction of Soluble and Insoluble Protein Fractions from Mouse Brains and Spinal Cords
    小鼠脑和脊髓中可溶性和不溶性蛋白质组分的提取
    作者:Oliver Wirths日期:08/05/2017,浏览量:22290,Q&A: 1
    [Abstract] The current protocol details the preparation of soluble and insoluble protein lysates from mouse brain or spinal cord samples. In detail, tissue homogenization and sequential protein extraction are described. This procedure yields soluble and insoluble protein extracts that can be further processed in down-stream applications like ELISA or Western ...