细胞生物学

分类

    现刊
    Quantitation of Secretory Granule Size in Drosophila Larval Salivary Glands
    果蝇幼虫唾液腺分泌颗粒大小的定量研究
    作者:Cheng-I J. Ma and Julie A. Brill日期:06/05/2021,浏览量:1312,Q&A: 0
    [Abstract]

    Maturation of secretory granules is a crucial process that ensures the bioactivity of cargo proteins undergoing regulated secretion. In Drosophila melanogaster, the larval salivary glands produce secretory granules that are up to four-fold larger in cross-sectional area after maturation. Therefore, we developed a live imaging microscopy approach

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    Fractionation of Ovarian Follicles and in vitro Oocyte Maturation and Ovulation Assay of Ciona intestinalis Type A
    A型玻璃海鞘卵泡分级分离、体外卵母细胞成熟和排卵检测
    作者:Shin Matsubara, Akira Shiraishi, Tomohiro Osugi, Tsuyoshi Kawada and Honoo Satake日期:04/05/2020,浏览量:2254,Q&A: 0
    [Abstract] Ascidians are the closest living relatives of vertebrates (Delsuc et al., 2006; Satoh et al., 2014) and are important for the evolutionary study of the ovarian follicle development including oocyte maturation and ovulation. However, neither the endogenous factors nor the molecular mechanisms underlying the oocyte maturation and ...
    Mouse Embryonic Tooth Germ Dissection and Ex vivo Culture Protocol
    小鼠胚胎牙胚分离和体外培养方案
    作者:Xue Han, Keigo Yoshizaki, Tian Tian, Kanako Miyazaki, Ichiro Takahashi and Satoshi Fukumoto日期:02/05/2020,浏览量:3195,Q&A: 1
    [Abstract] A tooth germ ex vivo organ culture allows visualization of its development in different stages, thus enabling investigation of the molecular mechanisms of regulatory factors. Tooth germs can be rapidly dissected from E13 mouse embryos and placed on cell culture inserts for observation of subsequent tooth germ development in a ...
    Sebinger Culture: A System Optimized for Morphological Maturation and Imaging of Cultured Mouse Metanephric Primordia
    Sebinger培养:一个用于小鼠后肾原基形态成熟和成像的优化系统
    作者:Mona Elhendawi and Jamie A. Davies日期:02/20/2018,浏览量:4511,Q&A: 0
    [Abstract] Here, we present a detailed protocol on setting up embryonic renal organ cultures using a culture method that we have optimised for anatomical maturation and imaging. Our culture method places kidney rudiments on glass in a thin film of medium, which results in very flat cultures with all tubules in the same image plane. For reasons not yet ...