生物化学

分类

    现刊
    Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Protein Vesicle Tracking in HeLa Cells
    用RUSH蛋白分选法追踪HeLa细胞中的蛋白囊泡
    作者:Natalia Pacheco-Fernandez, Mehrshad Pakdel and Julia von Blume日期:03/05/2021,浏览量:1869,Q&A: 0
    [Abstract]

    Monitoring vesicle trafficking is an excellent tool for the evaluation of protein dynamics in living cells. Such study is key for the understanding of protein sorting and secretion. Recent developments in microscopy, as well as new methodologies developed to study synchronized trafficking of proteins, allowed a better understanding of signaling,

    ...
    Karyopherin-β2 Inhibits and Reverses Aggregation and Liquid-liquid Phase Separation of the ALS/FTD-Associated Protein FUS
    核转运蛋白-β2抑制和ALS / FTD相关蛋白FUS的逆转聚集和液-液相分离
    作者:Emma Robinson, James Shorter and Lin Guo日期:08/20/2020,浏览量:1977,Q&A: 0
    [Abstract] The study of RNA-binding proteins (RBP) offers insight into the mechanisms of pathologic protein aggregation in neurodegenerative diseases. We developed a protocol for purifying an RBP FUS and a nuclear import receptor (NIR) Kapβ2 and testing the ability of Kapβ2 to mitigate FUS aggregation and liquid-liquid phase separation.
    Microtubule Seeded-assembly in the Presence of Poorly Nucleating Nucleotide Analogues
    存在不良成核核苷酸类似物的微管种晶组装
    作者:Siou Ku, Claire Heichette, Laurence Duchesne and Denis Chrétien日期:08/20/2020,浏览量:1465,Q&A: 0
    [Abstract] Microtubule dynamic instability is driven by the hydrolysis of the GTP bound to the β-subunit of the α-β tubulin heterodimer. Nucleotide analogues are commonly used to mimic the different steps of the tubulin GTPase cycle, but most of them are poor microtubule nucleators. Usually, microtubule assembly is seeded by guanylyl-(α, ...
    Split Nano Luciferase-based Assay to Measure Assembly of Japanese Encephalitis Virus
    基于分割纳米荧光素酶的乙型脑炎病毒组装检测
    作者:Simon Goto, Kotaro Ishida, Ryosuke Suzuki and Eiji Morita日期:05/05/2020,浏览量:1677,Q&A: 0
    [Abstract] Cells infected with flavivirus release various forms of infectious and non-infectious particles as products and by-products. Comprehensive profiling of the released particles by density gradient centrifugation is informative for understanding viral particle assembly. However, it is difficult to detect low-abundance minor particles in such ...
    Studying Protein Aggregation in the Context of Liquid-liquid Phase Separation Using Fluorescence and Atomic Force Microscopy, Fluorescence and Turbidity Assays, and FRAP
    利用荧光和原子力显微镜、荧光和浊度分析以及FRAP研究液-液相分离中的蛋白质聚集
    作者:W. Michael Babinchak and Witold K. Surewicz日期:01/20/2020,浏览量:4002,Q&A: 0
    [Abstract] Liquid-liquid phase separation (LLPS) underlies the physiological assembly of many membrane-less organelles throughout the cell. However, dysregulation of LLPS may mediate the formation of pathological aggregates associated with neurodegenerative diseases. Here, we present complementary experimental approaches to study protein aggregation within ...
    Purification of Globular Actin from Rabbit Muscle and Pyrene Fluorescent Assays to Investigate Actin Dynamics in vitro
    从兔肌肉组织中纯化球状肌动蛋白并利用芘荧光分析实验体外检测肌动蛋白的动态变化
    作者:He Sun, Yuanyuan Luo and Yansong Miao日期:12/05/2018,浏览量:3631,Q&A: 0
    [Abstract] Pyrene Fluorescent Assay is established to monitor the dynamic actin nucleation, elongation, capping and disassembly in vitro. This technique provides an easy handle procedure and straightforward visual data analysis. By coupling actin purification and polymerization assays in this protocol, the readers could quickly get the affordable ...
    Deoxycholate Fractionation of Fibronectin (FN) and Biotinylation Assay to Measure Recycled FN Fibrils in Epithelial Cells
    在上皮细胞中利用脱氧胆酸盐分离纤维粘连蛋白以及对回收的纤维粘连蛋白原纤维的生物素化分析
    作者:Archana Varadaraj, Carina Magdaleno and Karthikeyan Mythreye日期:08/20/2018,浏览量:3902,Q&A: 0
    [Abstract] Fibronectin (FN) is an extracellular matrix protein that is secreted by many cell types and binds predominantly to the cell surface receptor Integrin α5β1. Integrin α5β1 binding initiates the step-wise assembly of FN into fibrils, a process called fibrillogenesis. We and several others have demonstrated critical effects of fibrillogenesis on cell ...
    α-Synuclein Aggregation Monitored by Thioflavin T Fluorescence Assay
    通过硫磺素T荧光测定法监测核突触蛋白聚集
    作者:Michael M. Wördehoff and Wolfgang Hoyer日期:07/20/2018,浏览量:8302,Q&A: 1
    [Abstract] Studying the aggregation of amyloid proteins like α-synuclein in vitro is a convenient and popular tool to gain kinetic insights into aggregation as well as to study factors (e.g., aggregation inhibitors) that influence it. These aggregation assays typically make use of the fluorescence dye Thioflavin T as a sensitive ...
    Preparation of Amyloid Fibril Networks
    淀粉样纤维网络的制备
    作者:Mirren Charnley , Jay Gilbert, Owen G. Jones and Nicholas P. Reynolds日期:02/20/2018,浏览量:5835,Q&A: 0
    [Abstract] Networks of amyloid nanofibrils fabricated from common globular proteins such as lysozyme and β-lactoglobulin have material properties that mimic the extracellular microenvironment of many cell types. Cells cultured on such amyloid fibril networks show improved attachment, spreading and in the case of mesenchymal stem cells improved ...
    Characterization of Amyloid Fibril Networks by Atomic Force Microscopy
    通过原子力显微镜检查鉴定淀粉样蛋白原纤维网络特征
    作者:Mirren Charnley , Jay Gilbert, Owen G. Jones and Nicholas P. Reynolds日期:02/20/2018,浏览量:6056,Q&A: 0
    [Abstract] Dense networks of amyloid nanofibrils fabricated from common globular proteins adsorbed to solid supports can improve cell adhesion, spreading and differentiation compared to traditional flat, stiff 2D cell culture substrates like Tissue Culture Polystyrene (TCPS). This is due to the fibrous, nanotopographic nature of the amyloid fibril networks ...