微生物学

分类

    现刊
    PCR-mediated One-day Synthesis of Guide RNA for the CRISPR/Cas9 System
    CRISPR/Cas9系统的PCR介导的一日合成向导RNA
    作者:Naim Hassan, Farhana Easmin, Keisuke Ekino and Satoshi Harashima日期:07/05/2021,浏览量:1138,Q&A: 0
    [Abstract]

    Nowadays, CRISPR (clustered regularly interspaced short palindromic repeats) and the CRISPR-associated protein (Cas9) system play a major role in genome editing. To target the desired sequence of the genome successfully, guide RNA (gRNA) is indispensable for the CRISPR/Cas9 system. To express gRNA, a plasmid expressing the gRNA sequence is

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    Genomic Edition of Ashbya gossypii Using One-vector CRISPR/Cas9
    利用一个CRISPR/Cas9载体对棉囊阿舒氏酵母进行基因组编辑
    作者:Gloria Muñoz-Fernández, Alberto Jiménez and José Luis Revuelta日期:06/20/2020,浏览量:1683,Q&A: 0
    [Abstract] The CRISPR/Cas9 system is a novel genetic tool which allows the precise manipulation of virtually any genomic sequence. In this protocol, we use a specific CRISPR/Cas9 system for the manipulation of Ashbya gossypii. The filamentous fungus A. gossypii is currently used for the industrial production of riboflavin (vitamina B2). In ...
    CRISPR-Cas9 Genome Editing of Plasmodium knowlesi
    诺氏疟原虫CRISPR-Cas9基因编辑
    作者:Franziska Mohring, Melissa N. Hart, Avnish Patel, David A. Baker and Robert W. Moon日期:02/20/2020,浏览量:3074,Q&A: 1
    [Abstract] Plasmodium knowlesi is a zoonotic malaria parasite in Southeast Asia that can cause severe and fatal malaria in humans. The main hosts are Macaques, but modern diagnostic tools reveal increasing numbers of human infections. After P. falciparum, P. knowlesi is the only other malaria parasite capable of being maintained in ...
    Unbiased and Tailored CRISPR/Cas gRNA Libraries by Synthesizing Covalently-closed-circular (3Cs) DNA
    通过合成共价闭合环状(3Cs)DNA构建无偏和专用CRISPR / Cas gRNA文库
    作者:Martin Wegner, Koraljka Husnjak and Manuel Kaulich日期:01/05/2020,浏览量:4655,Q&A: 0
    [Abstract] Simplicity, efficiency and versatility of the CRISPR/Cas system greatly contributed to its rapid use in a broad range of fields. Applications of unbiased CRISPR/Cas screenings are increasing and thus there is a growing need for unbiased and tailored CRISPR/Cas gRNA libraries. Conventional methods for gRNA library generation apply PCR and cloning ...
    Single and Multiplexed Gene Editing in Ustilago maydis Using CRISPR-Cas9
    使用CRISPR-Cas9对玉米黑粉菌进行单个和多个基因的编辑
    作者:Mariana Schuster, Christine Trippel, Petra Happel, Daniel Lanver, Stefanie Reißmann and Regine Kahmann日期:07/20/2018,浏览量:5675,Q&A: 0
    [Abstract] The smut fungus Ustilago maydis is an established model organism for elucidating how biotrophic pathogens colonize plants and how gene families contribute to virulence. Here we describe a step by step protocol for the generation of CRISPR plasmids for single and multiplexed gene editing in U. maydis. Furthermore, we describe the ...