生物化学

分类

    现刊
    Photoactivable Cholesterol as a Tool to Study Interaction of Influenza Virus Hemagglutinin with Cholesterol
    利用光活化胆固醇研究流感病毒血凝素与胆固醇相互作用
    作者:Bodan Hu, Mohamed Rasheed Gadalla, Christoph Thiele and Michael Veit日期:02/20/2020,浏览量:2314,Q&A: 0
    [Abstract] Non-covalent binding of cholesterol to the transmembrane region of proteins affect their functionalities, but methods to prove such an interaction are rare. We describe our protocol to label the hemagglutinin (HA) of Influenza virus with a cholesterol derivative in living cells or with immunoprecipitated protein. We synthesized a “clickable” ...
    Liposome Flotation Assay for Studying Interactions Between Rubella Virus Particles and Lipid Membranes
    用于风疹病毒颗粒和脂膜相互作用研究的脂质体浮选分析法
    作者:Kyoko Saito, Noriyuki Otsuki, Makoto Takeda and Kentaro Hanada日期:08/20/2018,浏览量:6466,Q&A: 0
    [Abstract] Rubella virus (RuV) is an enveloped, positive-sense single-stranded RNA virus that is pathogenic to humans. RuV binds to the target cell via the viral envelope protein E1, but the specific receptor molecules on the target cell are yet to be fully elucidated. Here, we describe a protocol for liposome flotation assay to study direct interactions ...