微生物学

分类

    现刊
    An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated Protein
    利用芽殖酵母微管相关蛋白研究微管结合和微管交联的体外显微镜检测法
    作者:Yili Zhu, Weimin Tan and Wei-Lih Lee日期:12/05/2018,浏览量:4290,Q&A: 0
    [Abstract] In this protocol, we describe a simple microscopy-based method to assess the interaction of a microtubule-associated protein (MAP) with microtubules. The interaction between MAP and microtubules is typically assessed by a co-sedimentation assay, which measures the amount of MAP that co-pellets with microtubules by centrifugation, followed by ...