细胞生物学

分类

    现刊
    Construction and Validation of A Low-cost, Small-scale, Multiplex Continuous Culturing System for Microorganisms
    低成本、小规模、复合微生物连续培养系统的构建与验证
    作者:Lilit Tonoyan, Freddy Guihéneuf, Ruari Friel and Vincent O’Flaherty日期:11/05/2020,浏览量:1347,Q&A: 0
    [Abstract]

    This protocol details the construction of a simple, low-cost, small-scale, multiplex chemostat system designed for the continuous cultivation of microorganisms in suspension (i.e., bacteria, yeast, microalgae). The continuous culture device can operate at a working volume of 25 ml and allows the run of 8 chemostats in parallel by a single person.

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    A CRISPR Competition Assay to Identify Cancer Genetic Dependencies
    基于CRISPR竞争法鉴定癌症的遗传依赖性
    作者:Vishruth Girish and Jason M. Sheltzer日期:07/20/2020,浏览量:4272,Q&A: 0
    [Abstract] The CRISPR/Cas9 system is a powerful tool for genome editing, wherein the RNA-guided nuclease Cas9 can be directed to introduce double-stranded breaks (DSBs) at a targeted locus. In mammalian cells, these DSBs are typically repaired through error-prone processes, resulting in insertions or deletions (indels) at the targeted locus. Researchers can ...
    In vitro Screening of Antileishmanial Activity of Natural Product Compounds: Determination of IC50, CC50 and SI Values
    天然复合产物抗利什曼原虫活性的体外筛选:IC50、CC50和SI值的测定
    作者:Olga S. Koutsoni, Kalliopi Karampetsou and Eleni Dotsika日期:11/05/2019,浏览量:2466,Q&A: 0
    [Abstract] Neglected tropical diseases gain the scientific interest of numerous research programs in an attempt to achieve their effective control or elimination. In this attempt, more cutting-edge public health policies and research are needed for the discovery of new, safer and effective drugs originated from natural products. Here, we describe protocols ...
    Assessing Yeast Cell Survival Following Hydrogen Peroxide Exposure
    过氧化氢胁迫条件下酵母细胞存活率的评估
    作者:Khoa Tran and Erin M. Green日期:01/20/2019,浏览量:6642,Q&A: 0
    [Abstract] In the presence of oxidative stress, cellular defense systems that can detoxify reactive oxygen species are activated through multiple signaling cascades and transcriptional reprogramming. The budding yeast Saccharomyces cerevisiae has served as an excellent model for genetically-identifying factors important for the response to oxidative ...