微生物学

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    现刊
    COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR
    COVID-19样本汇集:从RNA提取到实时定量RT-PCR
    [Abstract]

    The COVID-19 pandemic requires mass screening to identify those infected for isolation and quarantine. Individually screening large populations for the novel pathogen, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is costly and requires a lot of resources. Sample pooling methods improve the efficiency of mass screening and consume

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    A Sensitive and Specific PCR-based Assay to Quantify Hepatitis B Virus Covalently Closed Circular (ccc) DNA while Preserving Cellular DNA
    一种在保留细胞DNA的同时定量检测乙型肝炎病毒共价闭合环状(ccc)DNA的灵敏、特异PCR方法
    作者:Benno Zehnder, Stephan Urban and Thomas Tu日期:04/20/2021,浏览量:2637,Q&A: 0
    [Abstract]

    Hepatitis B virus (HBV) is the major cause of liver diseases and liver cancer worldwide. After infecting hepatocytes, the virus establishes a stable episome (covalently closed circular DNA, or cccDNA) that serves as the template for all viral transcripts. Specific and accurate quantification of cccDNA is difficult because infected cells contain

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    Evaluation of the Sequence Variability within the PCR Primer/Probe Target Regions of the SARS-CoV-2 Genome
    SARS-CoV-2基因组PCR引物/探针靶区序列变异性评价
    作者:Kashif Aziz Khan and Peter Cheung日期:12/20/2020,浏览量:1831,Q&A: 0
    [Abstract] Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; initially named 2019-nCoV) is responsible for the recent coronavirus disease (COVID-19) pandemic, and polymerase chain reaction (PCR) is the current standard method for diagnosis from patient samples. As PCR assays are prone to sequence mismatches due to mutations in the viral genome, it ...
    Screening Method for CRISPR/Cas9 Inhibition of a Human DNA Virus: Herpes Simplex Virus
    人单纯疱疹病毒CRISPR/Cas9抑制作用的筛选方法
    [Abstract] The efficiency of cleavage of individual CRISPR/Cas9-sgRNAs remains difficult to predict based on the CRISPR target sequence alone. Different intracellular environments (dependent on cell type or cell cycle state for example) may affect sgRNA efficiency by altering accessibility of genomic DNA through DNA modifications such as epigenetic marks and ...
    Safe DNA-extraction Protocol Suitable for Studying Tree-fungus Interactions
    适于研究树-真菌互作的安全DNA提取方法
    作者:Susanna Keriö, Eeva Terhonen and Jared M. LeBoldus日期:06/05/2020,浏览量:3435,Q&A: 0
    [Abstract] We present a safe and low-cost method suitable for DNA extraction from mycelium and tree tissue samples. After sample preparation, the extraction takes about 60 min. Method performance was tested by extracting DNA from various tree tissue samples and from mycelium grown on solid and liquid media. DNA was extracted from juvenile and mature host ...
    Viral Double-Stranded RNA Detection by DNase I and Nuclease S1 digestions in Leishmania parasites
    在利什曼原虫中利用DNase I和 Nuclease S1酶解进行病毒双链RNA鉴定
    作者:Nathalie Isorce and Nicolas Fasel日期:05/05/2020,浏览量:1885,Q&A: 0
    [Abstract] Many RNA viruses are found in protozoan parasites. They can be responsible for more serious pathology or treatment failure. For the detection of viral double-stranded RNA (dsRNA), sequence-dependent and -independent methods are available, such as quantitative real-time PCR and immunofluorescence, dot blot, ELISA or sequencing. The technique ...
    Solid Phase PCR on 3D Microstructure ArrayChip for Pathogen Detection Application
    用于病原体检测的三维显微组织列阵中固相PCR
    作者:Krishna Kant and Tien Anh Ngo日期:08/05/2019,浏览量:3251,Q&A: 0
    [Abstract] Advanced free angle photolithography (FAPL) is presented for making 3D supercritical angle fluorescence (SAF) microstructures and transfer them on to polymeric chips using injection molding technique for low-cost microfluidic devices embedded with optical sensing structures. A solid phase polymerase chain reaction (SP-PCR) is used as model ...