生物化学

分类

    现刊
    Visualization of Host Cell Kinase Activation by Viral Proteins Using GFP Fluorescence Complementation and Immunofluorescence Microscopy
    利用GFP荧光互补和免疫荧光显微镜观察病毒蛋白对宿主细胞激酶的激活
    作者:Sherry T. Shu, Wing Fai Li and Thomas E. Smithgall日期:07/05/2021,浏览量:1420,Q&A: 0
    [Abstract]

    Non-receptor protein-tyrosine kinases regulate cellular responses to many external signals and are important drug discovery targets for cancer and infectious diseases. While many assays exist for the assessment of kinase activity in vitro, methods that report changes in tyrosine kinase activity in single cells have the potential to provide

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    Imaging Microtubules in vitro at High Resolution while Preserving their Structure
    保留微管结构同时在体外对其进行高分辨率成像
    作者:Camille Cuveillier, Yasmina Saoudi, Isabelle Arnal and Christian Delphin日期:04/05/2021,浏览量:752,Q&A: 0
    [Abstract]

    Microtubules (MT) are the most rigid component of the cytoskeleton. Nevertheless, they often appear highly curved in the cellular context and the mechanisms governing their overall shape are poorly understood. Currently, in vitro microtubule analysis relies primarily on electron microscopy for its high resolution and Total Internal Reflection

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    Simultaneous Imaging of Single Protein Size, Charge, and Binding Using A Protein Oscillation Approach
    利用蛋白质振荡方法同时成像单个蛋白质的大小、电荷和结合状态
    作者:Guangzhong Ma, Zijian Wan and Shaopeng Wang日期:03/05/2021,浏览量:2468,Q&A: 0
    [Abstract]

    Electrophoresis and Western blot are important tools in protein research for detection and identification of proteins. These traditional techniques separate the proteins based on size and charge differences and identify the proteins by antibody binding. Over the past decade, the emergence of single-molecule techniques has shown great potential in

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    Live Cell FRET Analysis of the Conformational Changes of Human P-glycoprotein
    人P-糖蛋白构象变化的活细胞FRET分析
    作者:Ryota Futamata, Noriyuki Kioka and Kazumitsu Ueda日期:02/20/2021,浏览量:2362,Q&A: 0
    [Abstract]

    The molecular mechanisms of P-glycoprotein (P-gp; also known as MDR1 or ABCB1) have been mainly investigated using artificial membranes such as lipid-detergent mixed micelles, artificial lipid bilayers, and membrane vesicles derived from cultured cells. Although these in vitro experiments help illustrate details about the molecular mechanisms of

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    Determination of Microtubule Lattice Parameters from Cryo-electron Microscope Images Using TubuleJ
    使用TubuleJ从冷冻电子显微镜图像确定微管晶格参数
    作者:Siou Ku, Cédric Messaoudi, Charlotte Guyomar, Charles Kervrann and Denis Chrétien日期:11/05/2020,浏览量:1282,Q&A: 0
    [Abstract]

    The α-β tubulin heterodimer undergoes subtle conformational changes during microtubule assembly. These can be modulated by external factors, whose effects on microtubule structure can be characterized on 2D views obtained by cryo-electron microscopy. Analysis of microtubule images is facilitated if they are straight enough to interpret

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    Protocols for Processing and Interpreting cryoEM Data Using Bsoft: A Case Study of the Retinal Adhesion Protein, Retinoschisin
    利用Bsoft处理和解释冷冻电镜数据方法:视网膜粘连蛋白,视网膜劈裂蛋白的实例研究
    作者:J. Bernard Heymann日期:01/20/2020,浏览量:2512,Q&A: 0
    [Abstract] The goal of cryoEM is to determine the structures of biomolecules from electron micrographs. In many cases the processing is straightforward and can be handled with routine protocols. In other cases, the properties and behavior of the specimen require adaptions to properly interpret the data. Here I describe the protocols for examining the higher ...
    Isolation and Imaging of His- and RFP-tagged Amyloid-like Proteins from Caenorhabditis elegans by TEM and SIM
    TEM和SIM技术用于秀丽隐杆线虫His-和RFP标记淀粉样蛋白的分离和成像
    作者:Amberley D. Stephens, Meng Lu and Gabriele S. Kaminski Schierle日期:11/05/2019,浏览量:2268,Q&A: 0
    [Abstract] In our recently published paper, we highlight that during normal aging of C. elegans age-dependent aggregates of proteins form and lead to functional decline of tissues. The protocol described here details the isolation of two proteins from C. elegans in their aggregated amyloid-like form, casein kinase I isoform alpha (KIN-19) ...