生物化学

分类

    现刊
    Identification of Intrinsic RNA Binding Specificity of Purified Proteins by in vitro RNA Immunoprecipitation (vitRIP)
    体外RNA免疫沉淀(vitRIP)鉴定纯化蛋白的RNA结合特异性
    作者:Marisa Müller, Tamas Schauer and Peter B. Becker日期:03/05/2021,浏览量:1354,Q&A: 0
    [Abstract]

    RNA-protein interactions are often mediated by dedicated canonical RNA binding domains. However, interactions through non-canonical domains with unknown specificity are increasingly observed, raising the question how RNA targets are recognized. Knowledge of the intrinsic RNA binding specificity contributes to the understanding of target

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    Plant ARGONAUTE Protein Immunopurification for Pathogen Cross Kingdom Small RNA Analysis
    植物ARGONAUTE蛋白免疫纯化用于病原体跨界小RNA分析
    作者:Florian Dunker, Bernhard Lederer and Arne Weiberg日期:02/05/2021,浏览量:1907,Q&A: 0
    [Abstract]

    Over the last decade, it has been noticed that microbial pathogens and pests deliver small RNA (sRNA) effectors into their host plants to manipulate plant physiology and immunity for infection, known as cross kingdom RNA interference. In this process, fungal and oomycete parasite sRNAs hijack the plant ARGONAUTE (AGO)/RNA-induced silencing complex

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    Fluorescent Polysome Profiling in Caenorhabditis elegans
    秀丽隐杆线虫荧光多聚体分析
    作者:Dan Shaffer and Jarod A Rollins日期:09/05/2020,浏览量:2456,Q&A: 0
    [Abstract] An important but often overlooked aspect of gene regulation occurs at the level of protein translation. Many genes are regulated not only by transcription but by their propensity to be recruited to actively translating ribosomes (polysomes). Polysome profiling allows for the separation of unbound 40S and 60S subunits, 80S monosomes, and actively ...
    Biochemical Pulldown of mRNAs and Long Noncoding RNAs from Cellular Lysates Coupled with Mass Spectrometry to Identify Protein Binding Partners
    通过细胞裂解液中mRNAs和长链非编码RNA的生化沉降结合质谱法鉴定蛋白结合物
    作者:Anca F. Savulescu, Stoyan Stoychev, Sipho Mamputha and Musa M. Mhlanga日期:06/05/2020,浏览量:2651,Q&A: 2
    [Abstract] RNA binding proteins (RBPs) interact with cellular mRNAs, controlling various steps throughout the lifetime of these transcripts, including transcription, cellular transport, subcellular localization, translation and degradation. In addition to binding mRNA transcripts, a growing number of RBPs are shown to bind long noncoding RNAs (lncRNAs), ...
    Real-time Fluorescence Measurement of Enterovirus Uncoating
    肠道病毒脱壳的实时荧光检测
    作者:Visa Ruokolainen, Mira Laajala and Varpu Marjomäki日期:04/05/2020,浏览量:1743,Q&A: 0
    [Abstract] Viruses need to open, i.e., uncoat, in order to release their genomes for efficient replication and translation. Especially for non-enveloped viruses, such as enteroviruses, the cues leading to uncoating are less well known. The status of the virus has previously been observed mainly by transmission electron microscopy using negative ...
    Ribonucleoprotein Immunoprecipitation (RIP) Analysis
    核糖核蛋白免疫沉淀分析
    作者:Jennifer L. Martindale, Myriam Gorospe and Maria L. Idda日期:01/20/2020,浏览量:4232,Q&A: 0
    [Abstract] RNAs and RNA-binding proteins (RBPs) can interact dynamically in ribonucleoprotein (RNP) complexes that play important roles in controlling gene expression programs. One of the powerful ways to investigate changes in the association of RNAs with an RBP of interest is by immunoprecipitation (IP) analysis of native RNPs. RIP (RNP ...
    In vitro RNA Cleavage Assays to Characterize IRE1-dependent RNA Decay
    通过体外RNA剪切检测IRE1依赖的RNA降解
    作者:G. Elif Karagöz, Jirka Peschek, Peter Walter and Diego Acosta-Alvear日期:07/20/2019,浏览量:4461,Q&A: 0
    [Abstract] The kinase/RNase IRE1 is a key effector of the cellular response to endoplasmic reticulum stress. The RNase activity of IRE1 can be measured in cells or in the test tube. Here we describe a protocol for the in vitro cleavage and analysis of RNA substrates of IRE1. The method consists of the in vitro transcription, purification ...
    Preparation of Sequencing RNA Libraries through Chemical Cross-linking Coupled to Affinity Purification (cCLAP) in Saccharomyces cerevisiae
    通过化学交联联合亲和纯化(cCLAP) 技术制备酿酒酵母RNA测序文库
    作者:Congwei Wang, Julie Weidner and Anne Spang日期:10/05/2018,浏览量:4261,Q&A: 0
    [Abstract] Ribonucleoprotein particles (mRNPs) are complexes consisting of mRNAs and RNA-binding proteins (RBPs) which control mRNA transcription localization, turnover, and translation. Some mRNAs within the mRNPs have been shown to undergo degradation or storage. Those transcripts can lack general mRNA elements, like the poly(A) tail or 5’ cap structure, ...
    Purification of RNA Mango Tagged Native RNA-protein Complexes from Cellular Extracts Using TO1-Desthiobiotin Fluorophore Ligand
    使用TO1-脱硫生物素荧光团配体从细胞提取物中纯化RNA Mango标记的天然RNA-蛋白质复合物
    作者:Shanker Shyam Sundhar Panchapakesan, Sunny C. Y. Jeng and Peter J. Unrau日期:04/05/2018,浏览量:5456,Q&A: 2
    [Abstract] A native purification strategy using RNA Mango for RNA based purification of RNA-protein complexes is described. The RNA Mango aptamer is first genetically engineered into the RNA of interest. RNA Mango containing complexes obtained from cleared cellular native extracts are then immobilized onto TO1-Desthiobiotin saturated streptavidin agarose ...
    Accurate, Streamlined Analysis of mRNA Translation by Sucrose Gradient Fractionation
    通过蔗糖梯度分级分离准确、高效分析mRNA的翻译
    作者:Soufiane Aboulhouda, Rachael Di Santo, Gabriel Therizols and David Weinberg日期:10/05/2017,浏览量:8343,Q&A: 0
    [Abstract] The efficiency with which proteins are produced from mRNA molecules can vary widely across transcripts, cell types, and cellular states. Methods that accurately assay the translational efficiency of mRNAs are critical to gaining a mechanistic understanding of post-transcriptional gene regulation. One way to measure translational efficiency is to ...