干细胞

分类

    现刊
    Ex vivo Tissue Culture Protocols for Studying the Developing Neocortex
    研究发育中的新皮质的体外组织培养方案
    作者:Takashi Namba, Christiane Haffner and Wieland B. Huttner日期:05/20/2021,浏览量:1867,Q&A: 0
    [Abstract]

    The size of the neocortex and its morphology are highly divergent across mammalian species. Several approaches have been utilized for the analysis of neocortical development and comparison among different species. In the present protocol (Note: This protocol requires basic knowledge of brain anatomy), we describe three ex vivo neocortical

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    Investigating Neural Stem Cell and Glioma Stem Cell Self-renewal Potential Using Extreme Limiting Dilution Analysis (ELDA)
    利用极端有限稀释分析法(ELDA)检测神经干细胞和胶质瘤干细胞的自我更新能力
    作者:Hong PT Nguyen, Paul M Daniel, Gulay Filiz and Theo Mantamadiotis日期:09/05/2018,浏览量:6031,Q&A: 0
    [Abstract] Glioma stem cells (GSC) grown as neurospheres exhibit similar characteristics to neural stem cells (NSC) grown as neurospheres, including the ability to self-renew and differentiate. GSCs are thought to play a role in cancer initiation and progression. Self-renewal potential of GSCs is thought to reflect many characteristics associated with ...
    Isolation, Culture and Differentiation of Adult Hippocampal Precursor Cells
    成年海马前体细胞的分离,培养和分化
    作者:Stefanie N. Bernas, Odette Leiter, Tara L. Walker and Gerd Kempermann日期:11/05/2017,浏览量:13617,Q&A: 0
    [Abstract] There are two neurogenic niches in the adult mammalian brain: the subventricular zone of the lateral ventricle and the subgranular zone of the hippocampal dentate gyrus. Cells from these areas can be isolated and maintained in vitro, using two different culture systems to assess their potential regarding proliferation and differentiation ...
    Explant Methodology for Analyzing Neuroblast Migration
    采用外植体方法分析神经母细胞迁移
    作者:Kirsty J. Dixon, Alisa Turbic, Ann M. Turnley and Daniel J. Liebl日期:05/05/2017,浏览量:7559,Q&A: 0
    [Abstract] The subventricular zone (SVZ) in the mammalian forebrain contains stem/progenitor cells that migrate through the rostral migratory stream (RMS) to the olfactory bulb throughout adulthood. SVZ-derived explant cultures provide a convenient method to assess factors regulating the intermediary stage of neural stem/progenitor cell migration. Here, we ...
    A Co-culture Model for Determining the Target Specificity of the de novo Generated Retinal Ganglion Cells
    使用共培养模型测定新生成的视网膜神经节细胞的靶点特异性
    作者:Pooja Teotia, Matthew J. Van Hook and Iqbal Ahmad日期:04/05/2017,浏览量:7024,Q&A: 0
    [Abstract] In glaucoma, the output neurons of the retina, the retinal ganglion cells (RGCs), progressively degenerate, leading to irreversible blindness (Ahram et al., 2015). The ex vivo stem cell method to replace degenerated RGCs remains a potentially viable approach (Levin et al., 2004). However, the success of the approach ...
    Neurosphere Co-culture Assay
    神经球共培养试验
    作者:Fanny Ehret, Steffen Vogler and Gerd Kempermann日期:08/05/2016,浏览量:11034,Q&A: 0
    [Abstract] The hippocampal niche is one of two areas in the brain where stem cells reside. In this neurogenic niche, stem cells can be found in close proximity to astrocytes and in contact with microvessels consisting of pericytes and endothelial cells. To study the regulatory interplay of this complex niche network in a simplified in vitro model, ...
    Neural Stem Cell Differentiation and Prion Infection
    神经干细胞分化和朊病毒感染
    作者:Yoshifumi Iwamaru, Takato Takenouchi and Hiroshi Kitani日期:03/20/2014,浏览量:9803,Q&A: 0
    [Abstract] Prion diseases are transmissible, fatal, neurodegenerative diseases in human and animals. The molecular basis of neurodegeneration in prion diseases is largely unclear. Developing a cellular model capable of monitoring prion-induced cytotoxicity would be a promising approach for better understanding the prion pathogenesis. One candidate cellular ...
    Isolation and Culture of Neurospheres for the Study of Pathogenesis of Prion Disease
    分离和培养神经球用于研究朊病毒病发病机理
    作者:Yoshifumi Iwamaru, Takato Takenouchi and Hiroshi Kitani日期:03/20/2014,浏览量:19873,Q&A: 0
    [Abstract] Neurosphere contains neural stem cells that are capable of self-renewal and multilineage differentiation including neurons, astrocytes, and oligodendrocytes (Gage, 2000). Cell culture model using differentiated neurosphere cultures are suggested to be a valuable tool for studying the pathogenesis of prion disease at the cellular level (Iwamaru et ...
    Primary Culture of SVZ-derived Progenitors Grown as Neurospheres
    室管膜下区(SVZ)源祖细胞生长为神经球的初代培养
    作者:Julien Vernerey, Karine Magalon and Pascale Durbec日期:08/20/2013,浏览量:10398,Q&A: 1
    [Abstract] SVZ-derived progenitors grown as neurospheres is a well-known model to study neural stem cell and progenitor functions such as proliferation, differentiation/self-renewal, and migration (Durbec and Rougon, 2001). This protocol is for preparing a culture of SVZ-derived progenitors from 8 early postnatal mouse brains (P0 to P3). One week after cell ...
    Neuronal Morphology Analysis
    神经元形态分析
    作者:Désirée R. M. Seib and Ana Martin-Villalba日期:08/05/2013,浏览量:10101,Q&A: 0
    [Abstract] This protocol describes how to visualize neuronal morphology and how to determine neuronal complexity of immature and mature hippocampal neurons in the mouse in vivo including tissue preparation, staining of brain sections and confocal cell analysis.