分子生物学

分类

    现刊
    A Gel-Based Assay for Probing Protein Translocation on dsDNA
    一种基于凝胶的检测dsDNA蛋白易位的方法
    作者:Christiane Brugger and Alexandra M. Deaconescu日期:07/20/2021,浏览量:1010,Q&A: 0
    [Abstract]

    Protein translocation on DNA represents the key biochemical activity of ssDNA translocases (aka helicases) and dsDNA translocases such as chromatin remodelers. Translocation depends on DNA binding but is a distinct process as it typically involves multiple DNA binding states, which are usually dependent on nucleotide binding/hydrolysis and are

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    Characterize the Interaction of the DNA Helicase PriA with the Stalled DNA Replication Fork Using Atomic Force Microscopy
    用原子力显微镜描述DNA解旋酶PriA与停止的DNA复制叉的相互作用
    作者:Yaqing Wang, Zhiqiang Sun, Piero R. Bianco and Yuri L. Lyubchenko日期:03/05/2021,浏览量:2305,Q&A: 0
    [Abstract]

    In bacteria, the restart of stalled DNA replication forks requires the DNA helicase PriA. PriA can recognize and remodel abandoned DNA replication forks, unwind DNA in the 3'-to-5' direction, and facilitate the loading of the helicase DnaB onto the DNA to restart replication. ssDNA-binding protein (SSB) is typically present at the abandoned forks,

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    Chromatin Immunoprecipitation (ChIP) to Assess Histone Marks in Auxin-treated Arabidopsis thaliana Inflorescence Tissue
    染色质免疫沉淀法(ChIP)检测生长素处理拟南芥花序组织中的组蛋白标记
    作者:André Kuhn and Lars Østergaard日期:12/05/2020,浏览量:1482,Q&A: 0
    [Abstract] Chromatin immunoprecipitation coupled with quantitative PCR (ChIP-qPCR) or high-throughput sequencing (ChIP-seq) has become the gold standard for the identification of binding sites of DNA binding proteins and the localization of histone modification on a locus-specific or genome-wide scale, respectively. ChIP experiments can be divided into seven ...
    In vitro Protein-DNA Binding Assay (AlphaScreen® Technology)
    蛋白质-DNA结合的体外检测法 (AlphaScreen® Technology)
    作者:Mika Nomoto, Yasuomi Tada and Hironaka Tsukagoshi日期:02/05/2019,浏览量:5138,Q&A: 0
    [Abstract] Identification of specific DNA binding sites of transcription factors is important in understanding their functions. Recent techniques allow us to investigate genome-wide in vivo binding positions by chromatin immunoprecipitation combined with high-throughput sequencing. However, to further explore the binding motifs of transcription ...
    Microtitre Plate Based Cell-SELEX Method
    基于微量滴定板的Cell-SELEX技术
    作者:Munish Shorie and Harmanjit Kaur日期:10/20/2018,浏览量:4654,Q&A: 0
    [Abstract] Aptamers have emerged as a novel category in the field of bioreceptors due to their wide applications ranging from biosensing to therapeutics. Several variations of their screening process, called SELEX have been reported which can yield sequences with desired properties needed for their final use. We report a facile microtiter plate-based ...
    ChIP-seq Experiment and Data Analysis in the Cyanobacterium Synechocystis sp. PCC 6803
    蓝藻集胞藻PCC 6803的ChIP-seq实验和数据分析
    作者:Joaquín Giner-Lamia, Miguel A. Hernández-Prieto and Matthias E. Futschik日期:06/20/2018,浏览量:9066,Q&A: 0
    [Abstract] Nitrogen is an essential nutrient for all living organisms. In cyanobacteria, a group of oxygenic photosynthetic bacteria, nitrogen homeostasis is maintained by an intricate regulatory network around the transcription factor NtcA. Although mechanisms controlling NtcA activity appear to be well understood, the sets of genes under its control (i.e. ...
    An Improved Method for Measuring Chromatin-binding Dynamics Using Time-dependent Formaldehyde Crosslinking
    一种改进的使用时间依赖性甲醛交联测定染色质结合动力学的方法
    作者:Elizabeth A. Hoffman, Hussain Zaidi, Savera J. Shetty, Stefan Bekiranov and David T. Auble日期:02/20/2018,浏览量:6467,Q&A: 0
    [Abstract] Formaldehyde crosslinking is widely used in combination with chromatin immunoprecipitation (ChIP) to measure the locations along DNA and relative levels of transcription factor (TF)-DNA interactions in vivo. However, the measurements that are typically made do not provide unambiguous information about the dynamic properties of these ...
    Chromatin Affinity Purification (ChAP) from Arabidopsis thaliana Rosette Leaves Using in vivo Biotinylation System
    使用体内生物素化系统进行拟南芥莲座叶染色质亲和纯化(ChAP)
    作者:Weronika Sura and Piotr A. Ziolkowski日期:01/05/2018,浏览量:6175,Q&A: 0
    [Abstract] Chromatin Affinity Purification (ChAP) is widely used to study chromatin architecture and protein complexes interacting with DNA. Here we present an efficient method for ChAP from Arabidopsis thaliana rosette leaves, in which in vivo biotinylation system is used. The chromatin is digested by Micrococcal Nuclease (MNase), hence ...
    Chromatin Immunoprecipitation Experiments from Whole Drosophila Embryos or Larval Imaginal Discs
    整个果蝇胚胎或幼虫成虫盘的染色质免疫沉淀实验
    作者:Vincent Loubiere, Anna Delest, Bernd Schuettengruber, Anne-Marie Martinez and Giacomo Cavalli日期:06/05/2017,浏览量:10134,Q&A: 1
    [Abstract] Chromatin Immunoprecipitation coupled either to qPCR (qChIP) or high-throughput sequencing (ChIP-Seq) has been extensively used in the last decades to identify the DNA binding sites of transcription factors or the localization of various histone marks along the genome. The ChIP experiment generally includes 7 steps: collection of biological ...
    Micro-chromatin Immunoprecipation (μChIP) Protocol for Real-time PCR Analysis of a Limited Amount of Cells
    可对限量细胞进行qPCR分析的微染色质免疫沉淀(μChIP)方法
    作者:Sébastien Gillotin and François Guillemot日期:06/20/2016,浏览量:7120,Q&A: 0
    [Abstract] Chromatin immunoprecipitation followed by deep sequencing (ChIP-Seq) is an important strategy to study gene regulation. When availability of cells is limited, however, it can be useful to focus on specific genes to investigate in depth the role of transcription factors or histone marks. Unfortunately, performing ChIP experiments to study ...