细胞生物学

分类

    现刊
    OrganoPlate Micro-fluidic Microvessel Culture and Analysis
    OrganoPlate微流控微血管培养与分析
    作者:Abidemi Junaid and Thomas Hankemeier日期:07/05/2021,浏览量:1451,Q&A: 0
    [Abstract]

    The endothelial cells from the microvasculature are key drivers and targets of inflammatory and thrombotic processes in microvascular diseases. The study of bioactive lipids in inflammatory processes has been largely based on two-dimensional endothelial cell cultures. Three-dimensional microvessels-on-a-chip provides an opportunity to monitor the

    ...
    Extraction and Quantification of Sphingolipids from Hemiptera Insects by Ultra-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry
    超高效液相色谱-串联质谱联用法提取和定量半翅目昆虫鞘脂质
    作者:Ni Wang, Xiaoxiao Shi, Chao Zhang, Wenwu Zhou and Zeng-Rong Zhu日期:02/20/2021,浏览量:2316,Q&A: 0
    [Abstract]

    Sphingolipids are major structural components of endomembranes and have also been described as an intracellular second messenger involved in various biological functions in all eukaryotes and a few prokaryotes. Ceramides (Cer), the central molecules of sphingolipids, have been depicted in cell growth arrest, cell differentiation, and apoptosis.

    ...
    Metabolomic and Lipidomic Analysis of Bone Marrow Derived Macrophages
    骨髓衍生巨噬细胞的代谢组学和脂质组学分析
    作者:Gretchen L. Seim, Steven V. John and Jing Fan日期:07/20/2020,浏览量:3491,Q&A: 0
    [Abstract] Macrophages are highly plastic immune cells that are capable of adopting a wide array of functional phenotypes in response to environmental stimuli. The changes in macrophage function are often supported and regulated by changes in cellular metabolism. Capturing a comprehensive picture of metabolism is vital for understanding the role of metabolic ...
    Isolation of Lipid Rafts from Cultured Mammalian Cells and Their Lipidomics Analysis
    哺乳动物细胞脂筏的分离及其脂质组学分析
    作者:Nigora Mukhamedova, Kevin Huynh, Hann Low, Peter J. Meikle and Dmitri Sviridov日期:07/05/2020,浏览量:2466,Q&A: 0
    [Abstract] Lipid rafts are distinct liquid-ordered domains of plasma membranes of most eukaryotic cells providing platform for signaling pathways. Lipid composition of rafts is critical for their structural integrity and for regulation of signaling pathways originating from rafts. Here we provide a protocol to isolate lipid rafts from cultured human and ...
    Lipid-exchange Rate Assay for Lipid Droplet Fusion in Live Cells
    活体细胞中脂滴融合的脂类交换速率检测
    作者:Jia Wang, Boon Tin Chua, Peng Li and Feng-Jung Chen日期:07/20/2019,浏览量:3832,Q&A: 0
    [Abstract] Lipid droplets (LDs) are central organelles in maintaining lipid homeostasis. Defective LD growth often results in the development of metabolic disorders. LD fusion and growth mediated by cell death–inducing DNA fragmentation factor alpha (DFFA)-like effector (CIDE) family proteins are crucial for various biological processes including unilocular ...
    Determining Oxidative Damage by Lipid Peroxidation Assay in Rat Serum
    利用脂质过氧化测定检测大鼠血清的氧化损伤
    作者:Qian Tang, Yu-Wen Su and Cory J. Xian日期:06/20/2019,浏览量:5591,Q&A: 0
    [Abstract] It has been well-established that malondialdehyde (MDA), which is generated during the process of lipid peroxidation, is a commonly known biomarker for oxidative stress. Therefore, the serum levels of MDA are detected by using the lipid peroxidation assay with commercially available kit to determine the induction of oxidative stress in rat models.
    Fluorescence HPLC Analysis of the in-vivo Activity of Glucosylceramide Synthase
    葡糖基神经酰胺合酶体内活性的荧光HPLC检测
    作者:Kartik R. Roy, Sachin K. Khiste, Zhijun Liu and Yong-Yu Liu日期:06/20/2019,浏览量:3836,Q&A: 0
    [Abstract] Almost all functions of cells or organs rely on the activities of cellular enzymes. Indeed, the in-vivo activities that directly represent the cellular effects of enzymes in live organs are critical importance to appreciate the roles enzymes play in modulating physiological or pathological processes, although assessments of such in-vivo ...
    Preparation of Lipid-Stripped Serum for the Study of Lipid Metabolism in Cell Culture
    用于细胞培养中脂质代谢研究的去脂质血清的制备
    作者:Aaron M. Hosios, Zhaoqi Li, Evan C. Lien and Matthew G. Vander Heiden日期:06/05/2018,浏览量:10378,Q&A: 0
    [Abstract] Studying lipid metabolism in cultured cells is complicated by the fact that cells are typically cultured in the presence of animal serum, which contains a wide, variable, and undefined variety of lipid species. Lipid metabolism can impact cell physiology, signaling, and proliferation, and the ability to culture cells in the absence of exogenous ...
    Flow Cytometric Quantification of Fatty Acid Uptake by Mycobacterium tuberculosis in Macrophages
    流式细胞术定量测定巨噬细胞中结核分枝杆菌对脂肪酸的摄取
    作者:Evgeniya V. Nazarova, Maria Podinovskaia, David G. Russell and Brian C. VanderVen日期:02/20/2018,浏览量:8683,Q&A: 0
    [Abstract] Mycobacterium tuberculosis (Mtb) has evolved to assimilate fatty acids from its host. However, until recently, there was no reliable way to quantify fatty acid uptake by the bacteria during host cell infection. Here we describe a new method to quantify fatty acid uptake by intracellular bacilli. We infect macrophages with Mtb ...
    Ex vivo Analysis of Lipolysis in Human Subcutaneous Adipose Tissue Explants
    人体皮下脂肪组织外植体脂解作用的离体分析
    作者:Pauline Decaunes, Anne Bouloumié, Mikael Ryden and Jean Galitzky日期:02/05/2018,浏览量:5604,Q&A: 0
    [Abstract] Most studies of human adipose tissue (AT) metabolism and functionality have been performed in vitro on isolated mature adipocyte or in situ using the microdialysis technique (Lafontan, 2012). However, these approaches have several limitations. The use of mature isolated adipocytes is limiting as adipocytes are not in their ...