微生物学

分类

    现刊
    Plasmodium cynomolgi Berok Growth Inhibition Assay by Thiol-reactive Probe Based Flow Cytometric Measurement
    硫醇反应探针流式细胞术检测食蟹猴疟原虫生长抑制
    作者:Jessica Jie Ying Ong, Bruce Russell and Jin-Hee Han日期:09/05/2021,浏览量:770,Q&A: 0
    [Abstract]

    The relapsing malaria species, Plasmodium vivax, is the most widely distributed and difficult-to-treat cause of human malaria. The merozoites of P. vivax preferentially invade ephemeral human CD71+ reticulocytes (nascent reticulocytes), thereby limiting the development of a robust continuous culture in vitro. Fortunately, P. vivax’s sister

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    Yeast Lipid Extraction and Analysis by HPTLC
    酵母脂质的HPTLC提取及分析
    作者:Dan Li, Zheng-Tan Zhang, Cheng-Wen He and Zhiping Xie日期:07/05/2021,浏览量:1229,Q&A: 0
    [Abstract]

    The diversity of lipid structures, properties, and combinations in biological tissues makes their extraction and analysis an experimental challenge. Accordingly, even for one of the simplest single-cellular fungi, the budding yeast (Saccharomyces cerevisiae), numerous extraction and analysis protocols have been developed to separate and quantitate

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    Sex-specific Separation of Plasmodium falciparum Gametocyte Populations
    恶性疟原虫配子细胞群体的性别特异性分离
    作者:Melanie C. Ridgway, Daniela Cihalova and Alexander G. Maier日期:06/05/2021,浏览量:1345,Q&A: 0
    [Abstract]

    Plasmodium falciparum is a unicellular eukaryotic parasite that causes malaria in humans. The parasite is spread by Anopheles mosquitoes after ingestion of sexual stage parasites known as gametocytes. Malaria transmission depends on parasites switching from the disease-causing asexual blood forms to male and female gametocytes. The current

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    Isolation of GFP-expressing Malarial Hypnozoites by Flow Cytometry Cell Sorting
    流式细胞术分离表达GFP的疟原虫催眠素
    作者:Annemarie Voorberg-van der Wel, Sam O. Hoffman and Clemens H. M. Kocken日期:05/05/2021,浏览量:1672,Q&A: 0
    [Abstract]

    Hypnozoites are dormant liver-stage parasites unique to relapsing malarial species, including the important human pathogen Plasmodium vivax, and pose a barrier to the elimination of malaria. Little is known regarding the biology of these stages, largely due to their inaccessible location. Hypnozoites can be cultured in vitro but these cultures

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    Purification of Rice Stripe Virus
    水稻条纹病毒纯化
    作者:Gang Lu, Min Yao, Yijun Zhou and Xiaorong Tao日期:03/20/2020,浏览量:1950,Q&A: 0
    [Abstract] Although many spherical and rod-shaped plant virus purification protocols are now available, only a few protocols on filamentous plant virus purification have been published. Here, we report a protocol for large-scale purification of Rice stripe virus (RSV) from RSV-infected rice tissues. RSV virions with high infectivity were first precipitated ...
    Experimental Setup for a Diffusion Bioreactor to Isolate Unculturable Soil Bacteria
    分离不可培养土壤细菌的扩散生物反应器实验装置
    作者:Dhiraj Kumar Chaudhary and Jaisoo Kim日期:10/05/2019,浏览量:3393,Q&A: 0
    [Abstract] Unculturable bacteria are those bacteria which proliferate in their native habitat but unable to grow or thrive in the normal laboratory media and conditions. The molecular techniques have revealed the significance of these uncultured bacteria in terms of their functional diversity and potential to produce secondary metabolites. To achieve these ...
    Yeast Single-cell RNA-seq, Cell by Cell and Step by Step
    单细胞酵母逐个单步RNA-seq操作方法
    [Abstract] Single-cell RNA-seq (scRNA-seq) has become an established method for uncovering the intrinsic complexity within populations. Even within seemingly homogenous populations of isogenic yeast cells, there is a high degree of heterogeneity that originates from a compact and pervasively transcribed genome. Research with microorganisms such as yeast ...
    Detachment Procedure of Bacteria from Atmospheric Particles for Flow-cytometry Counting
    从大气颗粒物中分离细菌用于流式细胞计数的步骤
    作者:Carolina M. Araya, Alberto Cazorla and Isabel Reche日期:06/20/2019,浏览量:3266,Q&A: 0
    [Abstract] The protocol separates bacteria from atmospheric particles, obtaining with greater precision their abundance in the atmospheric deposition. This procedure is similar to the one used to separate bacteria in streambed sediments. The detachment procedure consists of a chemical treatment with sodium pyrophosphate and Tween 20 and a physical treatment ...
    Long-term in vitro Culture of Cryptosporidium parvum
    长期体外培养小隐孢子虫
    作者:Nigel Yarlett and Mary Morada日期:08/05/2018,浏览量:5711,Q&A: 0
    [Abstract] Continuous in vitro growth of Cryptosporidium parvum has proved difficult and conventional in vitro culture techniques result in short-term (2-5 days) growth of the parasite resulting in thin-walled oocysts that fail to propagate using in vitro cultures, and do not produce an active infection using ...
    Alphavirus Purification Using Low-speed Spin Centrifugation
    低速旋转离心纯化甲病毒
    作者:Vamseedhar Rayaprolu, Jolene Ramsey, Joseph Che-Yen Wang and Suchetana Mukhopadhyay日期:03/20/2018,浏览量:8573,Q&A: 2
    [Abstract] Chemical and sedimentation procedures are used to purify virus particles. While these approaches are successful for wild-type viruses, they are often not feasible for purifying mutant viruses with assembly defects. We combined two published methods (Atasheva et al., 2013; Moller-Tank et al., 2013), to generate a protocol that ...