植物科学

分类

    现刊
    K+ Release Assay and K+ Measurement in Oocyte Assay
    卵母细胞K+释放量及K+水平测定
    作者:Hong Li日期:11/05/2020,浏览量:1274,Q&A: 0
    [Abstract] The Xenopus oocyte is a powerful system for the exogenous expression and functional characterization of plant membrane transport proteins. Until now, a number of potassium transporters and channels have been identified in oocytes expression system by the two-electrode voltage clamp technology. It is difficult to characterize K+ ...
    Visualization of Nitric Oxide, Measurement of Nitrosothiols Content, Activity of NOS and NR in Wheat Seedlings
    小麦幼苗中一氧化氮可视化以及亚硝基硫醇含量和NOS、NR活性测定
    [Abstract] Nitric oxide (NO), is a redox-active, endogenous signalling molecule involved in the regulation of numerous processes. It plays a crucial role in adaptation and tolerance to various abiotic and biotic stresses. In higher plants, NO is produced either by enzymatic or non-enzymatic reduction of nitrite and an oxidative pathway requiring a putative ...
    Fabrication and Use of the Dual-Flow-RootChip for the Imaging of Arabidopsis Roots in Asymmetric Microenvironments
    制造和使用dual-flow-RootChip在不对称微环境下进行拟南芥根组织成像
    [Abstract] This protocol provides a detailed description of how to fabricate and use the dual-flow-RootChip (dfRootChip), a novel microfluidic platform for investigating root nutrition, root-microbe interactions and signaling and development in controlled asymmetric conditions. The dfRootChip was developed primarily to investigate how plants roots interact ...
    Boron Uptake Assay in Xenopus laevis Oocytes
    在非洲爪蟾卵母细胞系统中进行硼的吸收分析实验
    作者:Sheliang Wang, Namiki Mitani-Ueno and Junpei Takano日期:03/05/2018,浏览量:5545,Q&A: 0
    [Abstract] Boron (B) is essential for plant growth and taken up by plant roots as boric acid. Under B limitation, B uptake and translocation in plants are dependent on the boric acid channels located in the plasma membrane. Xenopus leavis oocyte is a reliable heterologous expression system to characterize transport activities of boric acid channels ...
    Determination of Boron Content Using a Simple and Rapid Miniaturized Curcumin Assay
    姜黄素法简单快速测定低浓度硼含量
    作者:Thotegowdanapalya C. Mohan and Alexandra M. E. Jones日期:01/20/2018,浏览量:5693,Q&A: 0
    [Abstract] To determine boron quantity in soil, water and biological samples, several protocols are available. Colorimetric assays are the simplest and cheapest methods which can be used to determine boron concentration. However, published protocols do not give straightforward guidance for beginners to adopt these protocols for routine use in the laboratory. ...
    Quantifying the Capacity of Phloem Loading in Leaf Disks with [14C]Sucrose
    用[14C]蔗糖定量叶盘韧皮部装载能力
    作者:Umesh P Yadav, Aswad S Khadilkar, Mearaj A Shaikh, Robert Turgeon and Brian G Ayre日期:12/20/2017,浏览量:5067,Q&A: 0
    [Abstract] Phloem loading and transport of photoassimilate from photoautotrophic source leaves to heterotrophic sink organs are essential physiological processes that help the disparate organs of a plant function as a single, unified organism. We present three protocols we routinely use in combination with each other to assess (1) the relative rates of ...
    Assessing Long-distance Transport from Photosynthetic Source Leaves to Heterotrophic Sink Organs with [14C]CO2
    利用[14C]CO2评估从光合源叶到异养库器官的远距离运输
    作者:Umesh P Yadav, Aswad S Khadilkar, Mearaj A Shaikh, Robert Turgeon and Brian G Ayre日期:12/20/2017,浏览量:4347,Q&A: 0
    [Abstract] Phloem loading and transport of photoassimilate from photoautotrophic source leaves to heterotrophic sink organs are essential physiological processes that help the disparate organs of a plant function as a single, unified organism. We present three protocols we routinely use in combination with each other to assess (1) the relative rates of ...
    Assessing Rates of Long-distance Carbon Transport in Arabidopsis by Collecting Phloem Exudations into EDTA Solutions after Photosynthetic Labeling with [14C]CO2
    用[14C]CO2进行光合标记后通过EDTA溶液收集韧皮部渗出液来评估拟南芥长距离碳运输速率
    作者:Umesh P Yadav, Aswad S Khadilkar, Mearaj A Shaikh, Robert Turgeon and Brian G Ayre日期:12/20/2017,浏览量:4563,Q&A: 0
    [Abstract] Phloem loading and transport of photoassimilate from photoautotrophic source leaves to heterotrophic sink organs are essential physiological processes that help the disparate organs of a plant function as a single, unified organism. We present three protocols we routinely use in combination with each other to assess (1) the relative rates of ...
    Establishing a Symbiotic Interface between Cultured Ectomycorrhizal Fungi and Plants to Follow Fungal Phosphate Metabolism
    在培养的外生菌根真菌和植物之间建立共生界面以示踪真菌磷酸盐代谢
    [Abstract] In ectomycorrhizal plants, the fungal cells colonize the roots of their host plant to create new organs called ectomycorrhizae. In these new organs, the fungal cells colonize the walls of the cortical cells, bathing in the same apoplasm as the plant cells in a space named the ‘Hartig net’, where exchanges between the two partners take place. ...
    Nitrate Assay for Plant Tissues
    植物组织中的硝酸盐含量测定
    作者:Lufei Zhao and Yong Wang日期:01/20/2017,浏览量:13937,Q&A: 4
    [Abstract] Nitrogen is an essential macronutrient for plant growth and nitrate content in plants can reflect the nitrogen supply of soil. Here, we provide the salicylic acid method to evaluate the nitrate content in plant tissues. The method is reliable and stable, thus it can be a good choice for measurement of nitrate in plant tissues.