细胞生物学

分类

    现刊
    OrganoPlate Micro-fluidic Microvessel Culture and Analysis
    OrganoPlate微流控微血管培养与分析
    作者:Abidemi Junaid and Thomas Hankemeier日期:07/05/2021,浏览量:1236,Q&A: 0
    [Abstract]

    The endothelial cells from the microvasculature are key drivers and targets of inflammatory and thrombotic processes in microvascular diseases. The study of bioactive lipids in inflammatory processes has been largely based on two-dimensional endothelial cell cultures. Three-dimensional microvessels-on-a-chip provides an opportunity to monitor the

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    Generation and Maintenance of Homogeneous Human Midbrain Organoids
    同种人类中脑类器官的生成与维持
    作者:Henrik Renner, Martha Grabos, Hans R. Schöler and Jan M. Bruder日期:06/05/2021,浏览量:1828,Q&A: 0
    [Abstract]

    Three-dimensional cell cultures (“organoids”) promise to better recapitulate native tissue physiology than traditional 2D cultures and are becoming increasingly interesting for disease modeling and compound screening efforts. While a number of protocols for the generation of neural organoids have been published, most protocols require

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    A Robust Mammary Organoid System to Model Lactation and Involution-like Processes
    一个健康的乳腺有机样系统来模拟哺乳和退化样过程
    作者:Elsa Charifou, Jakub Sumbal, Zuzana Koledova, Han Li and Aurélie Chiche日期:04/20/2021,浏览量:2407,Q&A: 0
    [Abstract]

    The mammary gland is a highly dynamic tissue that changes throughout reproductive life, including growth during puberty and repetitive cycles of pregnancy and involution. Mammary gland tumors represent the most common cancer diagnosed in women worldwide. Studying the regulatory mechanisms of mammary gland development is essential for understanding

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    A Method to Efficiently Cryopreserve Mammalian Cells on Paper Platforms
    一种纸质平台高效冷冻保存哺乳动物细胞的方法
    作者:Muhammedin Deliorman, Pavithra Sukumar, Roaa Alnemari and Mohammad A. Qasaimeh日期:09/20/2020,浏览量:2111,Q&A: 0
    [Abstract] This protocol describes a simple method to cryopreserve mammalian cells within filter papers as an alternative to conventional slow-freezing approach. The method involves treating paper fibers with fibronectin, using low concentrations of the cryoprotectant dimethyl sulfoxide (DMSO), and slow freezing cells to -80 °C at a 1 °C min-1 ...
    Gene Expression and Genome Editing Systems by Direct Delivery of Macromolecules Into Rice Egg Cells and Zygotes
    大分子直接导入水稻卵细胞和受精卵的基因表达和基因组编辑系统
    作者:Erika Toda and Takashi Okamoto日期:07/20/2020,浏览量:1563,Q&A: 0
    [Abstract] Polyethylene glycol calcium (PEG-Ca2+)-mediated transfection allows rapid and efficient examination to analyze diverse cellular functions of genes of interest. In plant cells, macromolecules, such as DNA, RNA and protein, are delivered into protoplasts derived from somatic tissues or calli via PEG-Ca2+ transfection. To ...
    Model of Chemotherapy-associated Mucositis and Oral Opportunistic Infections
    化疗相关性粘膜炎与口腔机会性感染模型的建立
    作者:Takanori Sobue, Martinna Bertolini, Angela Thompson and Anna Dongari-Bagtzoglou日期:11/05/2019,浏览量:2323,Q&A: 1
    [Abstract] Oral mucositis is a common complication of cancer chemotherapy treatment. Because of the lack of relevant oral mucositis experimental models, it is not clear how chemotherapeutic agents injure the oral mucosa and if commensal microorganisms accelerate tissue damage. We developed an organotypic oral mucosa model that mimics cellular responses ...
    3D Organoid Formation from the Murine Salivary Gland Cell Line SIMS
    小鼠唾液腺SIMS细胞系的三维类器官形成
    作者:Harleen K. Athwal and Isabelle M. A. Lombaert日期:10/05/2019,浏览量:3588,Q&A: 0
    [Abstract] Salivary glands consist of multiple phenotypically and functionally unique cell populations, such as the acinar, ductal, and myoepithelial cells that help produce, modify, and secrete saliva (Lombaert et al., 2011). Identification of mechanisms and factors that regulate these populations has been of key interest, as salivary gland-related ...
    Reconstituting Breast Tissue with Organotypic Three-dimensional Co-culture of Epithelial and Stromal Cells in Discontinuous Extracellular Matrices
    通过不连续细胞外基质中上皮细胞和基质细胞三维共培养重建乳腺组织
    作者:Gang Ren, Vandana Sharma, Joshua Letson, Yashna Walia, Veani Fernando and Saori Furuta日期:10/05/2019,浏览量:2494,Q&A: 0
    [Abstract] Co-culture systems utilizing reconstituted or synthetic extracellular matrix (ECM) and micropatterning techniques have enabled the reconstruction of surface epithelial tissues. This technique has been utilized in the regeneration, disease modeling and drug screening of the surface epithelia, such as the skin and esophagus. On the other hand, the ...
    3D Co-culture System of Mouse Prostatic Wild-type Fibroblasts with Human Prostate Cancer Epithelial Cells
    小鼠前列腺野生型成纤维细胞和人前列腺癌上皮细胞的3D共培养系统
    作者:Veronica R. Placencio-Hickok, Manabu Kato and Neil A. Bhowmick日期:05/05/2019,浏览量:3281,Q&A: 0
    [Abstract] Heterogeneous prostatic carcinoma-associated fibroblasts (CAF) contribute to tumor progression. This was established using transgenic mouse models. Paracrine interactions between fibroblasts and epithelial cells were further interrogated using isolated 2D cell culture systems, but 3D culture systems currently being developed can better mimic ...
    A Functionally Robust Phenotypic Screen that Identifies Drug Resistance-associated Genes Using 3D Cell Culture
    利用3D细胞培养鉴定耐药相关基因的一种功能性稳健表型筛选
    作者:Sun-Young Lee and Mina J. Bissell日期:11/20/2018,浏览量:3799,Q&A: 0
    [Abstract] Drug resistance is a major obstacle in cancer treatment: A case in point is the failure of cancer patients to respond to tyrosine kinase inhibitors (TKI) of EGFR, a receptor that is highly expressed in many cancers. Identification of the targets and delineation of mechanisms of drug resistance remain major challenges. Traditional pharmacological ...