微生物学

分类

    现刊
    Defined Mutant Library Sequencing (DML-Seq) for Identification of Conditional Essential Genes
    确定突变体文库测序(DML-Seq)用于鉴定的条件必需基因
    作者:Shuai Shao, Lifan Wei, Feng Xia, Yuanxing Zhang and Qiyao Wang日期:03/05/2021,浏览量:1771,Q&A: 0
    [Abstract]

    Transposon insertion sequencing (TIS) is an emerging technique which utilizes a massive transposon mutant library to screen specific phenotype and determine the conditional essential genetic requirements for bacterial fitness under distinct conditions combined with high-throughput parallel sequencing technology. Compared with a massive mutant

    ...
    Preparation of Red Palm Weevil Rhynchophorus Ferrugineus (Olivier) (Coleoptera: Dryophthoridae) Germ-free Larvae for Host-gut Microbes Interaction Studies
    用于宿主肠道微生物相互作用研究的红棕象甲(稻铁甲虫)(鞘翅目:椰象鼻虫科)无菌幼虫的制备
    作者:Abrar Muhammad, Prosper Habineza, Youming Hou and Zhanghong Shi日期:12/20/2019,浏览量:2673,Q&A: 0
    [Abstract] Red palm weevil (RPW), Rhynchophorus ferrugineus Olivier, is a devastating pest of palm trees worldwide. RPW gut is colonized by diverse bacterial species which profoundly influence host development and nutritional metabolism. However, the molecular mechanisms behind the interactions between RPW and its gut microbiota remain mostly ...
    Delivery of the Cas9 or TevCas9 System into Phaeodactylum tricornutum via Conjugation of Plasmids from a Bacterial Donor
    通过细菌供体质粒接合转移的方法将Cas9或 TevCas9系统导入三角褐指藻
    作者:Helen Wang, Samuel S. Slattery, Bogumil J. Karas and David R. Edgell日期:08/20/2018,浏览量:5219,Q&A: 2
    [Abstract] Diatoms are an ecologically important group of eukaryotic microalgae with properties that make them attractive for biotechnological applications such as biofuels, foods, cosmetics and pharmaceuticals. Phaeodactylum tricornutum is a model diatom with defined culture conditions, but routine genetic manipulations are hindered by a lack of ...
    Random Insertional Mutagenesis of a Serotype 2 Dengue Virus Clone
    2型登革病毒克隆的随机插入诱变
    作者:Jeffrey W. Perry and Andrew W. Tai日期:08/20/2018,浏览量:3620,Q&A: 0
    [Abstract] Protein tagging is a powerful method of investigating protein function. However, modifying positive-strand RNA virus proteins in the context of viral infection can be particularly difficult as their compact genomes and multifunctional proteins mean even small changes can inactivate or attenuate the virus. Although targeted approaches to ...
    CRISPR/Cas Gene Editing of a Large DNA Virus: African Swine Fever Virus
    一种大DNA病毒(非洲猪瘟病毒)的CRISPR/Cas基因编辑
    作者:Manuel V. Borca, Keith A. Berggren, Elizabeth Ramirez-Medina, Elizabeth A. Vuono and Douglas P. Gladue日期:08/20/2018,浏览量:5950,Q&A: 0
    [Abstract] Gene editing of large DNA viruses, such as African swine fever virus (ASFV), has traditionally relied on homologous recombination of a donor plasmid consisting of a reporter cassette with surrounding homologous viral DNA. However, this homologous recombination resulting in the desired modified virus is a rare event. We recently reported the use of ...
    Method for CRISPR/Cas9 Mutagenesis in Candida albicans
    白色念珠菌的CRISPR/Cas9诱变方法
    作者:Neta Dean and Henry Ng日期:04/20/2018,浏览量:8403,Q&A: 0
    [Abstract] Candida albicans is the most prevalent and important human fungal pathogen. The advent of CRISPR as a means of gene editing has greatly facilitated genetic analysis in C. albicans. Here, we describe a detailed step-by-step procedure to construct and analyze C. albicans deletion mutants. This protocol uses plasmids that ...
    Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9
    使用CRISPR-Cas9在酵母中进行单步精确基因组编辑
    [Abstract] Genome modification in budding yeast has been extremely successful largely due to its highly efficient homology-directed DNA repair machinery. Several methods for modifying the yeast genome have previously been described, many of them involving at least two-steps: insertion of a selectable marker and substitution of that marker for the intended ...
    Design of Hybrid RNA Polymerase III Promoters for Efficient CRISPR-Cas9 Function
    设计合成RNA聚合酶III启动子以高效启动CRISPR-Cas9功能
    作者:Joshua Misa, Cory Schwartz and Ian Wheeldon日期:03/20/2018,浏览量:6471,Q&A: 0
    [Abstract] The discovery of the CRISPR-Cas9 system from Streptococcus pyogenes has allowed the development of genome engineering tools in a variety of organisms. A frequent limitation in CRISPR-Cas9 function is adequate expression levels of sgRNA. This protocol provides a strategy to construct hybrid RNA polymerase III (Pol III) promoters that ...
    Microbial Mutagenicity Assay: Ames Test
    微生物诱变分析:Ames试验
    作者:Urvashi Vijay, Sonal Gupta, Priyanka Mathur, Prashanth Suravajhala and Pradeep Bhatnagar日期:03/20/2018,浏览量:26473,Q&A: 2
    [Abstract] The Microbial mutagenicity Ames test is a bacterial bioassay accomplished in vitro to evaluate the mutagenicity of various environmental carcinogens and toxins. While Ames test is used to identify the revert mutations which are present in strains, it can also be used to detect the mutagenicity of environmental samples such as drugs, dyes, ...
    Conditional Knockdown of Proteins Using Auxin-inducible Degron (AID) Fusions in Toxoplasma gondii
    在刚地弓形虫中通过融合生长素诱导的降解因子(AID)进行蛋白质条件性敲减
    作者:Kevin M. Brown, Shaojun Long and L. David Sibley日期:02/20/2018,浏览量:11770,Q&A: 0
    [Abstract] Toxoplasma gondii is a member of the deadly phylum of protozoan parasites called Apicomplexa. As a model apicomplexan, there is a great wealth of information regarding T. gondii’s 8,000+ protein coding genes including sequence variation, expression, and relative contribution to parasite fitness. However, new tools are needed to ...