微生物学

分类

    现刊
    Evaluation of Toxicity with Brine Shrimp Assay
    通过卤虫实验进行毒性评价
    作者:Christina N. Banti and Sotiris K. Hadjikakou日期:01/20/2021,浏览量:2355,Q&A: 0
    [Abstract]

    The in vivo toxicity of new metallodrugs either as Small Bioactive Molecules (SBAMs) or Conjugates of Metals with Drugs (CoMeDs) or their hydrogels such as with hydroxyethyl-methacrylate (HEMA) (pHEMA@SBAMs or pHEMA@CoMeDs) are evaluated by the brine shrimp assay. Thus individuals of Artemia salina larvae are incubated in saline solutions with

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    Neutrophil Extracellular Trap Killing Assay of Candida albicans
    白念珠菌中性粒细胞胞外诱杀试验
    作者:Sheng-Yang Wu and Betty A. Wu-Hsieh日期:08/20/2020,浏览量:2216,Q&A: 0
    [Abstract] Fungal pathogen Candida albicans is one of the top leading causes of overall healthcare-associated bloodstream infections worldwide. Neutrophil is the major effector cell to clear C. albicans infection. Our study showed that mouse neutrophils utilize two independent mechanisms to kill C. albicans: one is CR3 downstream ...
    Quantification of Trypanosoma cruzi in Tissue and Trypanosoma cruzi Killing Assay
    组织中克氏锥虫的定量及克氏锥虫杀灭试验
    作者:Hisako Kayama, Shoko Kitada and Kiyoshi Takeda日期:11/20/2017,浏览量:5871,Q&A: 0
    [Abstract] Infection with Trypanosoma cruzi causes Chagas disease. The methods provided here allow for the quantification of T. cruzi in the liver, heart, and blood of intraperitoneally-infected mice and analysis of the killing activity of the cells infected with T. cruzi in vitro.
    Gentamicin Protection Assay to Determine Bacterial Survival within Macrophages
    庆大霉素保护试验测定细菌在巨噬细胞中的存活能力
    作者:Sargurunathan Subashchandrabose and Harry L.T. Mobley日期:09/20/2014,浏览量:19671,Q&A: 0
    [Abstract] Macrophages are key cells involved in orchestrating host defense against infections. Here, we describe the protocol for a bacterial killing assay in macrophages that can be adapted to any bacterial pathogen. Using this assay, we analyzed the survival of wild-type and mutant strains of Escherichia coli (E. coli) within RAW 264.7 ...
    Isolation of Neutralizing Antibody
    中和抗体的分离
    作者:Mayo Yasugi and Kazuyoshi Ikuta日期:12/20/2013,浏览量:7971,Q&A: 0
    [Abstract] Use of monoclonal antibodies (MAbs) is an established laboratory strategy for characterization of specific pathogens and their antigenicity. Especially, Human MAbs (HuMAbs) with neutralizing activity against specific virus could have potential therapeutic application, and provide significant information on human epitopes that could be important ...
    H2O2 Kill Assays of Planktonic Stationary Phase Bacteria
    H2O2杀灭浮游生物静止期细菌的分析
    作者:Malika Khakimova and Dao Nguyen日期:11/05/2013,浏览量:8747,Q&A: 0
    [Abstract] Stationary phase bacteria are highly tolerant to hydrogen peroxide. This protocol was developed to test the susceptibility to hydrogen peroxide killing in different Pseudomonas aeruginosa strains. This assay provides a reliable way to measure killing of stationary phase bacterial cells to hydrogen peroxide and can be adapted to test other ...