干细胞

分类

    现刊
    Isolation of Myofibres and Culture of Muscle Stem Cells from Adult Zebrafish
    成年斑马鱼肌纤维的分离和肌肉干细胞的培养
    作者:Massimo Ganassi, Peter S. Zammit and Simon M. Hughes日期:09/05/2021,浏览量:1222,Q&A: 0
    [Abstract]

    Skeletal muscles generate force throughout life and require maintenance and repair to ensure efficiency. The population of resident muscle stem cells (MuSCs), termed satellite cells, dwells beneath the basal lamina of adult myofibres and contributes to both muscle growth and regeneration. Upon exposure to activating signals, MuSCs proliferate to

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    Muscle Cryoinjury and Quantification of Regenerating Myofibers in Mice
    小鼠肌肉低温损伤及再生肌纤维的定量研究
    作者:Yori Endo, Mehran Karvar and Indranil Sinha日期:06/05/2021,浏览量:1417,Q&A: 0
    [Abstract]

    Cryoinjury, or injury due to freezing, is a method of creating reproducible, local injuries in skeletal muscle. This method allows studying the regenerative response following muscle injuries in vivo, thus enabling the evaluation of local and systemic factors that influence the processes of myofiber regeneration. Cryoinjuries are applicable to the

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    Isolation and Culture of Single Myofiber and Immunostaining of Satellite Cells from Adult C57BL/6J Mice
    成年C57BL/6J大鼠单个肌纤维的培养分离和卫星细胞的免疫染色
    作者:Shujie Chen, Hongrong Ding, Xiangping Yao and Liwei Xie日期:07/20/2019,浏览量:4959,Q&A: 0
    [Abstract] Myofiber isolation followed with ex vivo culture could recapitulate and visualize satellite cells (SCs) activation, proliferation, and differentiation. This approach could be taken to understand the physiology of satellite cells and the molecular mechanism of regulatory factors, in terms of the involvement of intrinsic factors over SCs ...
    Microarray, IPA and GSEA Analysis in Mice Models
    关于小鼠模型的微阵列,IPA和GSEA分析
    作者:Stephanie N. Oprescu, Katharine A. Horzmann, Feng Yue, Jennifer L. Freeman and Shihuan Kuang日期:09/05/2018,浏览量:6956,Q&A: 0
    [Abstract] This protocol details a method to analyze two tissue samples at the transcriptomic level using microarray analysis, ingenuity pathway analysis (IPA) and gene set enrichment analysis (GSEA). Methods such as these provide insight into the mechanisms underlying biological differences across two samples and thus can be applied to interrogate a variety ...
    A Method to Injure, Dissect and Image Indirect Flight Muscle of Drosophila
    果蝇间接飞行肌的损伤、解剖和成像方法
    作者:Kunal Chakraborty, K. VijayRaghavan and Rajesh Gunage日期:05/20/2018,浏览量:6397,Q&A: 0
    [Abstract] Inducing an injury specifically to Drosophila flight muscles is a difficult task, owing to the small size of the muscles and the presence of the cuticle. The protocol described below provides an easy and reproducible method to induce injury in the Drosophila flight muscles.
    Mouse Satellite Cell Isolation and Transplantation
    小鼠卫星细胞的分离和移植
    [Abstract] Satellite cell (SC) transplantation represents a powerful strategy to investigate SC biology during muscle regeneration. We described here a protocol for SC isolation from green fluorescent protein (GFP)-expressing mice and their transplantation into murine muscles. This procedure was originally used to assess the effects of the hormone unacylated ...
    Isolation of Primary Human Skeletal Muscle Cells
    原代人骨骼肌细胞的分离
    作者:Janelle M. Spinazzola and Emanuela Gussoni日期:11/05/2017,浏览量:6860,Q&A: 0
    [Abstract] Primary myoblast culture is a valuable tool in research of muscle disease, pathophysiology, and pharmacology. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic population by fluorescence-activated cell sorting (FACS). We also describe methods for assessing ...
    Isolation, Culturing, and Differentiation of Primary Myoblasts from Skeletal Muscle of Adult Mice
    成年小鼠骨骼肌的原代成肌细胞分离、培养和分化
    作者:Lubna Hindi, Joseph D. McMillan, Dil Afroze, Sajedah M. Hindi and Ashok Kumar日期:05/05/2017,浏览量:19635,Q&A: 4
    [Abstract] Myogenesis is a multi-step process that leads to the formation of skeletal muscle during embryonic development and repair of injured myofibers. In this process, myoblasts are the main effector cell type which fuse with each other or to injured myofibers leading to the formation of new myofibers or regeneration of skeletal muscle in adults. Many ...
    Quantitative 3D Time Lapse Imaging of Muscle Progenitors in Skeletal Muscle of Live Mice
    活小鼠骨骼肌中肌肉祖细胞的定量三维延时成像
    作者:Micah T. Webster, Tyler Harvey and Chen-Ming Fan日期:12/20/2016,浏览量:8254,Q&A: 0
    [Abstract] For non-optically clear mammalian tissues, it is now possible to use multi-photon microscopy to penetrate deep into the tissue and obtain detailed single cell images in a live animal, i.e., intravital imaging. This technique is in principle applicable to any fluorescently marked cell, and we have employed it to observe stem cells during ...
    Isolation, Culture, and Staining of Single Myofibers
    单肌纤维的分离、培养和染色
    作者:Yann Simon Gallot, Sajedah M. Hindi, Aman K. Mann and Ashok Kumar日期:10/05/2016,浏览量:11882,Q&A: 1
    [Abstract] Adult skeletal muscle regeneration is orchestrated by a specialized population of adult stem cells called satellite cells, which are localized between the basal lamina and the plasma membrane of myofibers. The process of satellite cell-activation, proliferation, and subsequent differentiation that occurs during muscle regeneration can be ...