生物化学

分类

    现刊
    Assessing Self-interaction of Mammalian Nuclear Proteins by Co-immunoprecipitation
    共免疫沉淀法评价哺乳动物核蛋白的自身相互作用
    作者:Claudia Cattoglio, Iryna Pustova, Xavier Darzacq, Robert Tjian and Anders S. Hansen日期:02/20/2020,浏览量:896,Q&A: 0
    [Abstract] Protein-protein interactions constitute the molecular foundations of virtually all biological processes. Co-immunoprecipitation (CoIP) experiments are probably the most widely used method to probe both heterotypic and homotypic protein-protein interactions. Recent advances in super-resolution microscopy have revealed that several nuclear proteins ...
    Immunohistochemical Identification of Muscle Fiber Types in Mice Tibialis Anterior Sections
    小鼠胫骨前肌切片中肌纤维类型的免疫组化鉴定
    作者:Vanitha V. Rao and Abhishek Mohanty日期:10/20/2019,浏览量:1146,Q&A: 0
    [Abstract] Mammalian skeletal muscle is a metabolically active tissue that is made up of different types of muscle fibers. These myofibers are made up of important contractile proteins that provide force during contraction of the muscle like actin and myosin. Murine myofibers have been classified into 4 types: Type I, Type IIa, Type IIb and Type IIX. Each ...
    A Highly Sensitive, Reproducible Assay for Determining 4-hydroxynonenal Protein Adducts in Biological Material
    生物材料中4-羟基壬烯醛与蛋白质加合物的高灵敏、可重复检测法
    作者:T. Blake Monroe and Ethan J. Anderson日期:10/05/2019,浏览量:662,Q&A: 0
    [Abstract] Oxidative stress is associated with numerous diseases, and markers of oxidative stress in biological material are becoming a mainstay of both experimental and clinical/epidemiological research. Lipid peroxidation is a major form of oxidative stress, but due to their rapid degradation and instability, lipid peroxides are notoriously difficult to ...
    Capillary Nano-immunoassay for Quantification of Proteins from CD138-purified Myeloma Cells
    毛细管纳米免疫实验定量分析经CD138筛选的骨髓瘤细胞蛋白
    作者:Irena Misiewicz-Krzeminska, Isabel Isidro and Norma C. Gutiérrez日期:06/20/2019,浏览量:2245,Q&A: 0
    [Abstract] Protein analysis in bone marrow samples from patients with multiple myeloma (MM) has been limited by the low concentration of proteins obtained after CD138+ cell selection. A novel approach based on capillary nano-immunoassay could make it possible to quantify dozens of proteins from each CD138+ purified MM sample in an ...
    Measurement of CD74 N-terminal Fragment Accumulation in Cells Treated with SPPL2a Inhibitor
    SPPL2a抑制剂处理细胞后的CD74N端片段聚集的检测
    作者:Rubén Martínez-Barricarte, Xiao-Fei Kong and Jean-Laurent Casanova日期:06/05/2019,浏览量:1488,Q&A: 0
    [Abstract] The recent discovery of human signal peptide peptidase-like 2a (SPPL2a) deficiency in humans revealed the toxicity associated with the accumulation of one of its substrates, CD74 N-terminal fragment (CD74-NTF), for certain type of dendritic cells (cDC2). We developed a two-step protocol for monitoring the accumulation of this molecule in different ...
    Quantification of Cutaneous Ionocytes in Small Aquatic Organisms
    小型水生生物皮肤离子细胞的定量分析
    作者:Garfield T. Kwan, Shane H. Finnerty, Nicholas C. Wegner and Martin Tresguerres日期:05/05/2019,浏览量:1507,Q&A: 0
    [Abstract] Aquatic organisms have specialized cells called ionocytes that regulate the ionic composition, osmolarity, and acid/base status of internal fluids. In small aquatic organisms such as fishes in their early life stages, ionocytes are typically found on the cutaneous surface and their abundance can change to help cope with various metabolic and ...
    Biofilm Assays on Fibrinogen-coated Silicone Catheters and 96-well Polystyrene Plates
    在纤维蛋白原包覆的硅胶导管和96孔聚苯乙烯板上进行生物膜检测
    作者:Cristina Colomer-Winter, José A. Lemos and Ana L. Flores-Mireles日期:03/20/2019,浏览量:2382,Q&A: 0
    [Abstract] Biofilm formation is a well-known bacterial strategy that protects cells from hostile environments. During infection, bacteria found in a biofilm community are less sensitive to antibiotics and to the immune response, often allowing them to colonize and persist in the host niche. Not surprisingly, biofilm formation on medical devices, such as ...
    Flow Cytometry Assay for Recycling of LFA-1 in T-lymphocytes
    流式细胞术检测T淋巴细胞中LFA-1的再循环
    作者:Katarzyna Potrzebowska, Janne Lehtonen, Malin Samuelsson and Lena Svensson日期:12/05/2018,浏览量:1921,Q&A: 0
    [Abstract] To enable cells to move forward, cell surface integrins are internalized into an endosomal compartment and subsequently intracellularly transported to be re-exposed at a new site on the cell membrane. Leukocytes are the fastest migrating cell type in the human body, which express the leukocyte-specific integrin LFA-1. Here, we describe a flow ...
    Separation and Visualization of Low Abundant Ubiquitylated Forms
    低丰度泛素化形式蛋白的分离和可视化
    作者:Ramona Schuster, Tânia Simões, Fabian den Brave and Mafalda Escobar-Henriques日期:11/20/2018,浏览量:1993,Q&A: 0
    [Abstract] In this protocol we describe the separation and visualization of ubiquitylated forms of the yeast mitofusin Fzo1 by Western blot. To this aim, we express HA-tagged Fzo1 in Saccharomyces cerevisiae, break the cells to extract a membrane-enriched fraction, solubilize the membranes using detergent and then specifically immunoprecipitate the ...
    Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA
    ELISA分析真核细胞中粘液生成增强并定量粘附的粘液
    作者:Christian Reuter and Tobias A. Oelschlaeger日期:06/20/2018,浏览量:4725,Q&A: 1
    [Abstract] The mucosal surfaces of the gastrointestinal, respiratory, reproductive, and urinary tracts, and the surface of the eye harbor a resident microflora that lives in symbiosis with their host and forms a complex ecosystem. The protection of the vulnerable epithelium is primarily achieved by mucins that form a gel-like structure adherent to the apical ...