生物化学

分类

    现刊
    Solubilization Method for Isolation of Photosynthetic Mega- and Super-complexes from Conifer Thylakoids
    从针叶树类囊体中分离光合巨复合体和超复合体的增溶方法
    作者:Pushan Bag, Wolfgang P. Schröder, Stefan Jansson and Domenica Farci日期:09/05/2021,浏览量:441,Q&A: 0
    [Abstract]

    Photosynthesis is the main process by which sunlight is harvested and converted into chemical energy and has been a focal point of fundamental research in plant biology for decades. In higher plants, the process takes place in the thylakoid membranes where the two photosystems (PSI and PSII) are located. In the past few decades, the evolution of

    ...
    Purification of Mitochondrial Ribosomes with the Translocase Oxa1L from HEK Cells
    用转位酶Oxa1L纯化HEK细胞线粒体核糖体
    作者:Hanting Yang and Nirupa Desai日期:08/05/2021,浏览量:955,Q&A: 0
    [Abstract]

    Mitochondrial ribosomes (mitoribosomes) perform protein synthesis inside mitochondria, the organelles responsible for energy conversion and adenosine triphosphate (ATP) production in eukaryotic cells. To investigate their functions and structures, large-scale purification of intact mitoribosomes from mitochondria-rich animal tissues or HEK cells

    ...
    Purification of Recombinant Wild Type and Mutant Ryanodine Receptors Expressed in HEK293 Cells
    HEK293细胞表达的重组野生型和突变型Ryanodine受体的纯化
    作者:Yifan Hu, Kavita A. Iyer, Ashok R. Nayak, Nagomi Kurebayashi, Takashi Murayama and Montserrat Samsó日期:08/05/2021,浏览量:801,Q&A: 0
    [Abstract]

    High quantities of purified ryanodine receptor (RyR), a large (2.26 MDa) intracellular homotetrameric membrane protein, can be obtained from heterologous expression in HEK293 cells and used for structure determination by cryo-EM. The advantage of using recombinant protein is that the variability due to post-translational modifications can be

    ...
    Cell-free Synthesis of Correctly Folded Proteins with Multiple Disulphide Bonds: Production of Fungal Hydrophobins
    有多重二硫化物键的正确折叠蛋白质的无细胞合成:真菌疏水性化合物的生产
    作者:Rezwan Siddiquee and Ann H Kwan日期:05/20/2021,浏览量:2327,Q&A: 0
    [Abstract]

    Cell-free synthesis is a powerful technique that uses the transcriptional and translational machinery extracted from cells to create proteins without the constraints of living cells. Here, we report a cell-free protein production protocol using Escherichia coli lysate (Figure 1) to successfully express a class of proteins (known as hydrophobins)

    ...
    Expression and Purification of the Human Cation-chloride Cotransporter KCC1 from HEK293F Cells for Structural Studies
    从HEK293F细胞中人K-Cl转运蛋白KCC1的表达和纯化用于结构研究
    作者:Si Liu and Jiangtao Guo日期:04/05/2021,浏览量:768,Q&A: 0
    [Abstract]

    Cation-chloride cotransporters (CCCs) mediate the coupled, electroneutral symport of cations such as Na+ and/or K+ with chloride across membrane. Among CCCs family, K-Cl cotransporters (KCC1-KCC4) extrude intracellular Cl- by the transmembrane K+ gradient. In humans, these KCCs play vital roles in the physiology of the nervous system and kidney.

    ...
    Expression and Purification of Yeast-derived GPCR, Gα and Gβγ Subunits for Structural and Dynamic Studies
    重组酵母的GPCR、Gα和Gβγ亚基的表达和纯化,用于结构和动力学研究
    作者:Wenjie Zhao, Xudong Wang and Libin Ye日期:02/20/2021,浏览量:3523,Q&A: 0
    [Abstract]

    In the last several years, as evidence of a surged number of GPCR-G complex structures, the expressions of GPCRs and G proteins for structural biology have achieved tremendous successes, mostly in insect and mammalian cell systems, resulting in more than 370 structures of over 70 GPCRs have been resolved. However, the challenge remains,

    ...
    Purification of Cytosolic Phospholipase A2α C2-domain after Expression in Soluble Form in Escherichia coli
    可溶性磷脂酶A2αC2结构域在大肠杆菌中表达后的纯化
    [Abstract]

    Previous expression/purification strategies for cytosolic phospholipase A2α C2-domain in Escherichia coli have relied on refolded protein recovered from inclusion bodies and sometimes containing C-terminal Cys139Ala and Cys141Ser substitutions to eliminate potential refolding complications induced by Cys residues. The protocol presented

    ...
    Reverse Phase-high-performance Liquid Chromatography (RP-HPLC) Analysis of Globin Chains from Human Erythroid Cells
    人类红细胞珠蛋白链的反相高效液相色谱(RP-HPLC)分析
    作者:Anne Chalumeau, Giacomo Frati, Elisa Magrin and Annarita Miccio日期:01/20/2021,浏览量:1270,Q&A: 0
    [Abstract]

    β-hemoglobinopathies are severe genetic disorders characterized either by the abnormal synthesis of the adult β-globin chains of the hemoglobin (Hb) tetramer (βS-globin chains) in sickle cell disease (SCD) or by the reduced β-globin production in β-thalassemia. The identification and quantification of globin chains are

    ...
    Preparation of Bacterial Outer Membrane Vesicles for Characterisation of Periplasmic Proteins in Their Native Environment
    细菌外膜囊泡的制备及其原生环境中周质蛋白的表征
    作者:Johannes Thoma and Björn M. Burmann日期:12/20/2020,浏览量:1673,Q&A: 0
    [Abstract]

    Bacterial outer membrane vesicles (OMVs) are naturally formed by budding from the outer membrane of Gram-negative bacteria. OMVs consist of a lipid bilayer identical in composition to the original outer membrane and contain periplasmic content within their lumen. Enriched with specific envelope proteins, OMVs make for an excellent native-like

    ...
    Cleavable Affinity Purification (Cl-AP): A One-step Procedure to Affinity Purify Protein Complexes
    CI-AP:一种亲和纯化蛋白质复合物的一步法
    作者:Ammarah Tariq, Lucy Green, Christian Soeller and James G. Wakefield日期:11/20/2020,浏览量:1890,Q&A: 0
    [Abstract]

    Cleavable Affinity Purification (Cl-AP) uses a tripartite system of Protein-A-Streptavidin beads and nanobodies, coupled with a biotinylated, thiol-cleavable linker, providing one-step affinity purification from lysates of tissues expressing tagged proteins. This technique allows fluorescent versions of mitotic protein complexes to be isolated

    ...