生物化学

分类

    现刊
    Self-organization Assay for Min Proteins of Escherichia coli in Micro-droplets Covered with Lipids
    脂质微滴中大肠杆菌Min蛋白的自组织分析
    作者:Shunshi Kohyama, Kei Fujiwara, Natsuhiko Yoshinaga and Nobuhide Doi日期:03/20/2020,浏览量:2581,Q&A: 0
    [Abstract] The Min system determines the cell division plane of bacteria. As a cue of spatiotemporal regulation, the Min system uses wave propagation of MinD protein (Min wave). Therefore, the reconstitution of the Min wave in cell-sized closed space will lead to the creation of artificial cells capable of cell division. The Min waves emerge via coupling ...
    Isolation, BODIPY Labeling and Uptake of Exosomes in Hepatic Stellate Cells
    肝星状细胞中外泌体的分离、BODIPY标记和摄取
    作者:Shubham Shrivastava日期:12/05/2017,浏览量:8648,Q&A: 1
    [Abstract] Exosomes have emerged as an important mediator of intercellular communication. They are present in extracellular milieu and therefore, easily accessible by neighboring or distant cells. They carry mRNA, microRNAs and proteins within their vesicles and once internalized by recipient cells; they can modulate multiple signaling pathways with ...
    Xanthoferrin Siderophore Estimation from the Cell-free Culture Supernatants of Different Xanthomonas Strains by HPLC
    HPLC法估测不同黄单胞菌菌株培养的无细胞上清液中黄单胞菌铁载体
    作者:Sheo Shankar Pandey, Prashantee Singh, Biswajit Samal, Raj Kumar Verma and Subhadeep Chatterjee日期:07/20/2017,浏览量:6781,Q&A: 0
    [Abstract] Xanthomonads can scavenge iron from the extracellular environment by secreting the siderophores, which are synthesized by the proteins encoded by xss (Xanthomonas siderophore synthesis) gene cluster. The siderophore production varies among xanthomonads in response to a limited supply of iron where Xanthomonas ...
    Loading of Extracellular Vesicles with Chemically Stabilized Hydrophobic siRNAs for the Treatment of Disease in the Central Nervous System
    将化学稳定的疏水siRNA加载到细胞外囊泡中用于治疗中枢神经系统疾病
    作者:Reka A. Haraszti, Andrew Coles, Neil Aronin, Anastasia Khvorova and Marie-Cécile Didiot日期:06/20/2017,浏览量:6848,Q&A: 0
    [Abstract] Efficient delivery of oligonucleotide therapeutics, i.e., siRNAs, to the central nervous system represents a significant barrier to their clinical advancement for the treatment of neurological disorders. Small, endogenous extracellular vesicles were shown to be able to transport lipids, proteins and RNA between cells, including neurons. ...
    Root Aliphatic Suberin Analysis Using Non-extraction or Solvent-extraction Methods
    不萃取或溶剂萃取法分析根脂肪族木栓质
    作者:Camille Delude, Sollapura J. Vishwanath, Owen Rowland and Frédéric Domergue日期:06/20/2017,浏览量:7632,Q&A: 1
    [Abstract] Here we describe both non-extraction and solvent-extraction methods for root aliphatic suberin analysis. The non-extraction method is fast as roots are directly depolymerized using acidic transmethylation. However, suberin aliphatic components are isolated together with all the other acyl chains making up the lipids (e.g., membranes) ...
    Procedure for Rhamnolipids Quantification Using Methylene-Blue
    使用亚甲蓝进行鼠李糖脂量化的方法
    作者:Tsiry Rasamiravaka, Olivier M. Vandeputte and Mondher El Jaziri日期:04/05/2016,浏览量:13271,Q&A: 2
    [Abstract] Rhamnolipids produced by Pseudomonas aeruginosa (P. aeruginosa) represent a group of biosurfactants with various applications (e.g., bioremediation of oil spills, cosmetics, detergents and cleaners). The commonly used colorimetric methods for rhamnolipid quantification, including anthrone, phenol−sulfuric acid and ...
    Protocol for Macrophage Depletion from Mice
    清除小鼠巨噬细胞的方法
    作者:Zuoxiang Xiao and Qun Jiang日期:06/05/2014,浏览量:16990,Q&A: 0
    [Abstract] Macrophage depletion has been used extensively to study autoimmune disease and more recently in tumor models. The clodronate-containing liposomes will be recognized as foreign particles and get engulfed by macrophages upon dosing into the animal by the chosen routes. Consequently, macrophages that have engulfed liposomes will all be destroyed by ...
    Preparation of Outer Membrane Vesicle from Escherichia coli
    从大肠杆菌分离外膜囊泡
    [Abstract] Outer membrane vesicles (OMVs) are spherical bilayered phospholipids of 20-200 nm in size produced from all Gram-negative bacteria and Gram-positive bacteria investigated to date. Previous biochemical and proteomic studies have revealed that the Gram-negative bacteria-derived OMVs are composed of various components like outer membrane proteins, ...
    Analysis of Flower Cuticular Waxes and Cutin Monomers
    花表面角质层蜡份和角质单体的分析
    作者:Anna Smirnova, Jana Leide and Markus Riederer日期:09/20/2013,浏览量:8506,Q&A: 0
    [Abstract] Here we describe procedures for the flower cuticular waxes extraction, modification and subsequent qualitative and quantitative analysis by gas-chromotography-mass spectrometry (GC-MS) and gas-chromotography with flame ionization detector (GC-FID), accordingly. To characterize flower cutin monomers two experimental setup are described: (i) ...
    Wax Analysis of Stem and Rosette Leaves in Arabidopsis thaliana
    拟南芥茎和莲座叶的蜡质层分析
    作者:Tegan M Haslam and Ljerka Kunst日期:06/05/2013,浏览量:12373,Q&A: 1
    [Abstract] The primary aerial surfaces of all land plants are coated by a lipidic cuticle, which restricts non-stomatal water loss and protects the plant from pathogens, herbivores, and ultraviolet radiation. The cuticle is made up of two components: cutin, a polymer of hydroxy- and epoxy- long-chain fatty acid derivatives and glycerol, and cuticular waxes, ...