Expansion of Airway Basal Cells and Generation of Polarized Epithelium
气道基底细胞扩增和极化上皮细胞生成
[Abstract] Airway basal stem cells are the progenitor cells within the airway that exhibit the capacity to self-renew and give rise to multiple types of differentiated airway epithelial cells. This stem cell-derived epithelium displays organized architecture with functional attributes of the airway mucosa. A protocol has been developed to culture and expand ...
Microbial Mutagenicity Assay: Ames Test
微生物诱变分析：Ames试验
[Abstract] The Microbial mutagenicity Ames test is a bacterial bioassay accomplished in vitro to evaluate the mutagenicity of various environmental carcinogens and toxins. While Ames test is used to identify the revert mutations which are present in strains, it can also be used to detect the mutagenicity of environmental samples such as drugs, dyes, ...
Preparation of Amyloid Fibril Networks
淀粉样纤维网络的制备
[Abstract] Networks of amyloid nanofibrils fabricated from common globular proteins such as lysozyme and β-lactoglobulin have material properties that mimic the extracellular microenvironment of many cell types. Cells cultured on such amyloid fibril networks show improved attachment, spreading and in the case of mesenchymal stem cells improved ...
Exit from Pluripotency Assay of Mouse Embryonic Stem Cells
小鼠胚胎干细胞退出多能性测定实验
[Abstract] A novel method to assess the dissolution of the core pluripotency transcription-factor circuit of mouse Embryonic Stem Cells (mESCs) has been developed (Ying et al., 2003; Betschinger et al., 2013). In order to efficiently identify genes essential for the break-down of the pluripotency network in mutant mESCs with proliferation ...
Isolation of Murine Alveolar Type II Epithelial Cells
小鼠肺泡II型上皮细胞分离
作者：Fan Sun,
Gutian Xiao and
Zhaoxia Qu，
日期：05/20/2017，
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[Abstract] We have optimized a protocol for isolation of alveolar type II epithelial cells from mouse lung. Lung cell suspensions are prepared by intratracheal instillation of dispase and agarose followed by mechanical disaggregation of the lungs. Alveolar type II epithelial cells are purified from these lung cell suspensions through magnetic-based negative ...