细胞生物学

分类

    现刊
    Expansion of Airway Basal Cells and Generation of Polarized Epithelium
    气道基底细胞扩增和极化上皮细胞生成
    作者:Hannah Levardon, Lael M. Yonker, Bryan P. Hurley and Hongmei Mou日期:06/05/2018,浏览量:9127,Q&A: 1
    [Abstract] Airway basal stem cells are the progenitor cells within the airway that exhibit the capacity to self-renew and give rise to multiple types of differentiated airway epithelial cells. This stem cell-derived epithelium displays organized architecture with functional attributes of the airway mucosa. A protocol has been developed to culture and expand ...
    Microbial Mutagenicity Assay: Ames Test
    微生物诱变分析:Ames试验
    作者:Urvashi Vijay, Sonal Gupta, Priyanka Mathur, Prashanth Suravajhala and Pradeep Bhatnagar日期:03/20/2018,浏览量:26995,Q&A: 2
    [Abstract] The Microbial mutagenicity Ames test is a bacterial bioassay accomplished in vitro to evaluate the mutagenicity of various environmental carcinogens and toxins. While Ames test is used to identify the revert mutations which are present in strains, it can also be used to detect the mutagenicity of environmental samples such as drugs, dyes, ...
    Preparation of Amyloid Fibril Networks
    淀粉样纤维网络的制备
    作者:Mirren Charnley , Jay Gilbert, Owen G. Jones and Nicholas P. Reynolds日期:02/20/2018,浏览量:6021,Q&A: 0
    [Abstract] Networks of amyloid nanofibrils fabricated from common globular proteins such as lysozyme and β-lactoglobulin have material properties that mimic the extracellular microenvironment of many cell types. Cells cultured on such amyloid fibril networks show improved attachment, spreading and in the case of mesenchymal stem cells improved ...
    A Bioreactor Method to Generate High-titer, Genetically Stable, Clinical-isolate Human Cytomegalovirus
    能产生高滴度、遗传稳定、临床分离人巨细胞病毒的一种生物反应器方法
    [Abstract] Human cytomegalovirus (HCMV) infection is a major cause of morbidity and mortality in transplant patients and a leading cause of congenital birth defects (Saint Louis, 2016). Vaccination and therapeutic studies often require scalable cell culture production of wild type virus, represented by clinical isolates. Obtaining sufficient stocks of ...
    Preparation of Primary Cultures of Embryonic Rat Hippocampal and Cerebrocortical Neurons
    胚胎大鼠海马和脑皮质神经元原代培养物的制备
    [Abstract] This protocol aims at standardizing the procedure to obtain primary cultures of hippocampal and cerebrocortical neurons for in vitro experiments. Cultures should be prepared from cells isolated during embryonic development when neuronal precursor cells are not yet fully differentiated. This helps increasing the quality and quantity of ...
    Exit from Pluripotency Assay of Mouse Embryonic Stem Cells
    小鼠胚胎干细胞退出多能性测定实验
    作者:Daniel Cirera-Salinas and Constance Ciaudo日期:08/20/2017,浏览量:6837,Q&A: 0
    [Abstract] A novel method to assess the dissolution of the core pluripotency transcription-factor circuit of mouse Embryonic Stem Cells (mESCs) has been developed (Ying et al., 2003; Betschinger et al., 2013). In order to efficiently identify genes essential for the break-down of the pluripotency network in mutant mESCs with proliferation ...
    Quantification of Chlorophyll as a Proxy for Biofilm Formation in the Cyanobacterium Synechococcus elongatus
    定量测定蓝藻细长聚球藻中作为生物膜形成代用指标的叶绿素
    作者:Eleonora Sendersky, Ryan Simkovsky, Susan S. Golden and Rakefet Schwarz日期:07/20/2017,浏览量:8343,Q&A: 0
    [Abstract] A self-suppression mechanism of biofilm development in the cyanobacterium Synechococcus elongatus PCC 7942 was recently reported. These studies required quantification of biofilms formed by mutants impaired in the biofilm-inhibitory process. Here we describe in detail the use of chlorophyll measurements as a proxy for biomass accumulation ...
    Culturing Bacteria from Caenorhabditis elegans Gut to Assess Colonization Proficiency
    利用秀丽隐杆线虫肠道培养细菌以评估其定植能力
    [Abstract] Determining an accurate count of intestinal bacteria from Caenorhabditis elegans is one critical way to assess colonization proficiency by a given bacteria. This can be accomplished by culturing appropriate dilutions of worm gut bacteria on selective or differential agarized media. Because of the high concentration of bacteria in gut ...
    Isolation of Murine Alveolar Type II Epithelial Cells
    小鼠肺泡II型上皮细胞分离
    作者:Fan Sun, Gutian Xiao and Zhaoxia Qu日期:05/20/2017,浏览量:9092,Q&A: 0
    [Abstract] We have optimized a protocol for isolation of alveolar type II epithelial cells from mouse lung. Lung cell suspensions are prepared by intratracheal instillation of dispase and agarose followed by mechanical disaggregation of the lungs. Alveolar type II epithelial cells are purified from these lung cell suspensions through magnetic-based negative ...
    Isolation and Cultivation of Primary Brain Endothelial Cells from Adult Mice
    从成年小鼠分离和培养原代脑内皮细胞
    [Abstract] Brain endothelial cells are the major building block of the blood-brain barrier. To study the role of brain endothelial cells in vitro, the isolation of primary cells is of critical value. Here, we describe a protocol in which vessel fragments are isolated from adult mice. After density centrifugation and mild digestion of the fragments, ...