An Inexpensive Imaging Platform to Record and Quantitate Bacterial Swarming
    作者:Weijie Chen, Sridhar Mani and Jay X. Tang日期:09/20/2021,浏览量:314,Q&A: 0

    Bacterial swarming refers to a rapid spread, with coordinated motion, of flagellated bacteria on a semi-solid surface (Harshey, 2003). There has been extensive study on this particular mode of motility because of its interesting biological and physical relevance, e.g., enhanced antibiotic resistance (Kearns, 2010) and turbulent collective motion

    Microscopic Detection of ASC Inflammasomes in Bone Marrow Derived Macrophages Post Stimulation
    作者:Longjun Li, Rudi Mao and Xiaopeng Qi日期:09/05/2021,浏览量:698,Q&A: 0

    An inflammasome is an intracellular multiprotein complex that plays important roles in host defense and inflammatory responses. Inflammasomes are typically composed of the adaptor protein apoptosis-associated speck-like protein containing a CARD (ASC), cytoplasmic sensor protein, and the effector protein pro-caspase-1. ASC assembly into a protein

    Tomato Stem Injection for the Precise Assessment of Ralstonia solanacearum Fitness in Planta
    作者:Yaru Wang, Liu Xian, Gang Yu and Alberto P. Macho日期:08/20/2021,浏览量:480,Q&A: 0

    Ralstonia solanacearum is a soil-borne pathogen with worldwide distribution that causes bacterial wilt disease in more than 250 plant species. R. solanacearum invades plants through the roots, reaches the vascular system, and colonizes the whole plant by moving through the xylem, where it eventually replicates rapidly, causing plant death. Usual

    Tracking the Subcellular Localization of Surface Proteins in Staphylococcus aureus by Immunofluorescence Microscopy
    作者:Salvatore J. Scaffidi, Mac A. Shebes and Wenqi Yu日期:05/20/2021,浏览量:3356,Q&A: 0

    Surface proteins of Staphylococcus aureus and other Gram-positive bacteria play essential roles in bacterial colonization and host-microbe interactions. Surface protein precursors containing a YSIRK/GXXS signal peptide are translocated across the septal membrane at mid-cell, anchored to the cell wall peptidoglycan at the cross-wall compartment,

    Assessing Swarming of Aerobic Bacteria from Human Fecal Matter
    作者:Arjun S. Byju, Deeti Patel, Weijie Chen and Sridhar Mani日期:05/05/2021,浏览量:1560,Q&A: 0

    Swarming – swift movement across a surface via flagella propulsion – is a unique property of many bacteria. The role of swarming, particularly among bacterial populations of the human gut microbiome, is not yet fully understood; although, it is becoming an area of increased scientific and clinical inquiry. To further characterize

    Measuring Cytosolic Translocation of Mycobacterium marinum in RAW264.7 Macrophages with a CCF4-AM FRET Assay
    用CCF4-AM FRET法检测RAW264.7巨噬细胞中海洋分枝杆菌的细胞质移位
    作者:Javier Aguilera and Jianjun Sun日期:04/20/2021,浏览量:1488,Q&A: 0
    [Abstract] The CCF4-AM Förster resonance energy transfer (FRET) assay is a sensitive approach to measure bacterial cytosolic translocation in live cells. The FRET pair hydroxycoumarin (donor) and fluorescein (acceptor) are linked by a CCF4-AM β-lactam ring, the substrate of β-lactamase. The exogenously added, neutral charged-FRET reagent can diffuse across ...
    Measurements of Root Colonized Bacteria Species
    作者:Vílchez Juan Ignacio, Yu Yang, Dian Yi and Huiming Zhang日期:04/05/2021,浏览量:2175,Q&A: 0

    Root-associated bacteria are able to influence plant fitness and vigor. A key step in understanding the belowground plant-bacteria interactions is to quantify root colonization by the bacteria of interest. Probably, genetic engineering with fluorescence markers is the most powerful way to monitor bacterial strains in plant. However, this could

    A Spectrofluorophotometrical Method Based on Fura-2-AM Probe to Determine Cytosolic Ca2+ Level in Pseudomonas syringae Complex Bacterial Cells

    Calcium signaling is an emerging mechanism by which bacteria respond to environmental cues. To measure the intracellular free-calcium concentration in bacterial cells, [Ca2+]i, a simple spectrofluorometric method based on the chemical probe Fura 2-acetoxy methyl ester (Fura 2-AM) is here presented using Pseudomonad bacterial cells. This is an

    Assay for Assessing Mucin Binding to Bacteria and Bacterial Proteins
    作者:Lubov S. Grigoryeva, Saima Rehman, Richard C. White, James A. Garnett and Nicholas P. Cianciotto日期:03/05/2021,浏览量:1584,Q&A: 0

    Legionella pneumophila, a Gram-negative bacterium and the causative agent of Legionnaires’ disease, exports over 300 effector proteins/virulence factors, through its type II (T2SS) and type IV secretion systems (T4SS). One such T2SS virulence factor, ChiA, not only functions as a chitinase, but also as a novel mucinase, which we believe aids

    Defined Mutant Library Sequencing (DML-Seq) for Identification of Conditional Essential Genes
    作者:Shuai Shao, Lifan Wei, Feng Xia, Yuanxing Zhang and Qiyao Wang日期:03/05/2021,浏览量:1967,Q&A: 0

    Transposon insertion sequencing (TIS) is an emerging technique which utilizes a massive transposon mutant library to screen specific phenotype and determine the conditional essential genetic requirements for bacterial fitness under distinct conditions combined with high-throughput parallel sequencing technology. Compared with a massive mutant