微生物学

分类

    现刊
    Tracking the Subcellular Localization of Surface Proteins in Staphylococcus aureus by Immunofluorescence Microscopy
    用免疫荧光显微镜追踪金黄色葡萄球菌表面蛋白的亚细胞定位
    作者:Salvatore J. Scaffidi, Mac A. Shebes and Wenqi Yu日期:05/20/2021,浏览量:1279,Q&A: 0
    [Abstract]

    Surface proteins of Staphylococcus aureus and other Gram-positive bacteria play essential roles in bacterial colonization and host-microbe interactions. Surface protein precursors containing a YSIRK/GXXS signal peptide are translocated across the septal membrane at mid-cell, anchored to the cell wall peptidoglycan at the cross-wall compartment,

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    Assessing Swarming of Aerobic Bacteria from Human Fecal Matter
    人类粪便中需氧细菌群集的评估
    作者:Arjun S. Byju, Deeti Patel, Weijie Chen and Sridhar Mani日期:05/05/2021,浏览量:457,Q&A: 0
    [Abstract]

    Swarming – swift movement across a surface via flagella propulsion – is a unique property of many bacteria. The role of swarming, particularly among bacterial populations of the human gut microbiome, is not yet fully understood; although, it is becoming an area of increased scientific and clinical inquiry. To further characterize

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    Measuring Cytosolic Translocation of Mycobacterium marinum in RAW264.7 Macrophages with a CCF4-AM FRET Assay
    用CCF4-AM FRET法检测RAW264.7巨噬细胞中海洋分枝杆菌的细胞质移位
    作者:Javier Aguilera and Jianjun Sun日期:04/20/2021,浏览量:645,Q&A: 0
    [Abstract] The CCF4-AM Förster resonance energy transfer (FRET) assay is a sensitive approach to measure bacterial cytosolic translocation in live cells. The FRET pair hydroxycoumarin (donor) and fluorescein (acceptor) are linked by a CCF4-AM β-lactam ring, the substrate of β-lactamase. The exogenously added, neutral charged-FRET reagent can diffuse across ...
    Measurements of Root Colonized Bacteria Species
    根定植细菌种类的测量
    作者:Vílchez Juan Ignacio, Yu Yang, Dian Yi and Huiming Zhang日期:04/05/2021,浏览量:921,Q&A: 0
    [Abstract]

    Root-associated bacteria are able to influence plant fitness and vigor. A key step in understanding the belowground plant-bacteria interactions is to quantify root colonization by the bacteria of interest. Probably, genetic engineering with fluorescence markers is the most powerful way to monitor bacterial strains in plant. However, this could

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    A Spectrofluorophotometrical Method Based on Fura-2-AM Probe to Determine Cytosolic Ca2+ Level in Pseudomonas syringae Complex Bacterial Cells
    基于Fura-2-AM探针的荧光光度法测定丁香假单胞菌复合菌胞内Ca2+水平
    [Abstract]

    Calcium signaling is an emerging mechanism by which bacteria respond to environmental cues. To measure the intracellular free-calcium concentration in bacterial cells, [Ca2+]i, a simple spectrofluorometric method based on the chemical probe Fura 2-acetoxy methyl ester (Fura 2-AM) is here presented using Pseudomonad bacterial cells. This is an

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    Assay for Assessing Mucin Binding to Bacteria and Bacterial Proteins
    评估粘蛋白与细菌和细菌蛋白结合的实验
    作者:Lubov S. Grigoryeva, Saima Rehman, Richard C. White, James A. Garnett and Nicholas P. Cianciotto日期:03/05/2021,浏览量:1175,Q&A: 0
    [Abstract]

    Legionella pneumophila, a Gram-negative bacterium and the causative agent of Legionnaires’ disease, exports over 300 effector proteins/virulence factors, through its type II (T2SS) and type IV secretion systems (T4SS). One such T2SS virulence factor, ChiA, not only functions as a chitinase, but also as a novel mucinase, which we believe aids

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    Defined Mutant Library Sequencing (DML-Seq) for Identification of Conditional Essential Genes
    确定突变体文库测序(DML-Seq)用于鉴定的条件必需基因
    作者:Shuai Shao, Lifan Wei, Feng Xia, Yuanxing Zhang and Qiyao Wang日期:03/05/2021,浏览量:701,Q&A: 0
    [Abstract]

    Transposon insertion sequencing (TIS) is an emerging technique which utilizes a massive transposon mutant library to screen specific phenotype and determine the conditional essential genetic requirements for bacterial fitness under distinct conditions combined with high-throughput parallel sequencing technology. Compared with a massive mutant

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    Pea Aphid Rearing, Bacterial Infection and Hemocyte Phagocytosis Assay
    豌豆蚜虫饲养、细菌感染及血细胞吞噬功能测定
    作者:Li Ma, Lu Liu and Zhiqiang Lu日期:12/20/2020,浏览量:923,Q&A: 0
    [Abstract]

    Insects rely on the simple but effective innate immune system to combat infection. Cellular and humoral responses are interconnected and synergistic in insects’ innate immune system. Phagocytosis is one major cellular response. It is difficult to collect clean hemolymph from the small insect like pea aphid. Here, we provide a practicable

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    Isolation and CryoTEM of Phages Infecting Bacterial Wine Spoilers
    感染葡萄酒腐败细菌的噬菌体分离与冷冻电镜观察
    作者:Amel Chaïb, Marion Decossas, Cécile Philippe, Olivier Claisse, Olivier Lambert and Claire Le Marrec日期:11/05/2020,浏览量:1022,Q&A: 0
    [Abstract]

    With the objective to isolate phages infecting wine bacterial spoilers, we designed a method for the isolation and purification of phages infecting grape-associated bacteria. The method proved successful to isolate GC1 tectivirus infecting the acetic acid bacterium Gluconobacter cerinus. The isolated phage represents a new genus within the

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    Murine Acute Pneumonia Model of Pseudomonas aeruginosa Lung Infection
    铜绿假单胞菌肺部感染的小鼠急性肺炎模型
    作者:Xiaolei Pan and Weihui Wu日期:11/05/2020,浏览量:1011,Q&A: 0
    [Abstract] Animal infection models play significant roles in studying bacterial pathogenic mechanisms, host pathogen interaction as well as evaluating drug and vaccine efficacies. We have been utilizing an acute pneumonia model to study bacterial colonization in lungs and assess virulence to the host by determination of bacterial loads and survival assays, ...