微生物学

分类

    现刊
    Generating Three-dimensional Human Granulomas in vitro to Study Mycobacterium tuberculosis-host Interaction
    通过体外生成三维人体肉芽肿研究结核分枝杆菌与宿主的相互作用
    作者:Ainhoa Arbués, Michael Kammüller and Damien Portevin日期:11/20/2020,浏览量:1569,Q&A: 0
    [Abstract] Granulomas are organized multicellular structures that constitute the hallmark of an infection by the human pathogen Mycobacterium tuberculosis (Mtb). A better understanding of the complex host-Mtb interactions within the granuloma’s environment may lead to new therapeutic or preventive tools to improve the control of the ...
    Candida albicans Agar Invasion Assays
    白念珠菌琼脂侵入实验
    作者:Shamoon Naseem, Lois M. Douglas and James B. Konopka日期:08/20/2020,浏览量:2333,Q&A: 0
    [Abstract] The ability of the human fungal pathogen Candida albicans to disseminate into tissues is promoted by a switch from budding to invasive hyphal growth. This morphological transition is stimulated by multiple environmental factors that can vary at different sites of infection. To identify genes that promote invasive growth, C. albicans ...
    Differential Fractionation of Erythrocytes Infected by Plasmodium berghei
    感染伯氏疟原虫红细胞的差异分离
    作者:Bénédicte Gnangnon, Véronique Peucelle and Christine Pierrot日期:06/05/2020,浏览量:1782,Q&A: 0
    [Abstract] The study of host/pathogen interactions at the cellular level during Plasmodium intra-erythrocytic cycle requires differential extraction techniques aiming to analyze the different compartments of the infected cell. Various protocols have been proposed in the literature to study specific compartments and/or membranes in the infected ...
    Model of Chemotherapy-associated Mucositis and Oral Opportunistic Infections
    化疗相关性粘膜炎与口腔机会性感染模型的建立
    作者:Takanori Sobue, Martinna Bertolini, Angela Thompson and Anna Dongari-Bagtzoglou日期:11/05/2019,浏览量:2551,Q&A: 1
    [Abstract] Oral mucositis is a common complication of cancer chemotherapy treatment. Because of the lack of relevant oral mucositis experimental models, it is not clear how chemotherapeutic agents injure the oral mucosa and if commensal microorganisms accelerate tissue damage. We developed an organotypic oral mucosa model that mimics cellular responses ...
    Intracellular Invasion and Killing Assay to Investigate the Effects of Binge Alcohol Toxicity in Murine Alveolar Macrophages
    研究酗酒酒精毒性对小鼠肺泡巨噬细胞影响的细胞内侵袭和杀伤试验
    作者:Victor Jimenez Jr and Fernando P Monroy日期:01/20/2019,浏览量:4115,Q&A: 0
    [Abstract] Alcohol consumption has diverse and well-documented effects on the human immune system and its ability to defend against infective agents. While pulmonary related infections can occur in healthy humans, binge alcohol use is recognized as a major health risk factor (Nelson et al., 1991). Although binge alcohol consumption has been ...
    High-throughput Microscopic Analysis of Salmonella Invasion of Host Cells
    高通量显微分析沙门氏菌入侵寄主细胞
    作者:Jakub Voznica, Jost Enninga and Virginie Stévenin日期:09/20/2018,浏览量:4314,Q&A: 0
    [Abstract] Salmonella is a Gram-negative bacterium causing a gastro-enteric disease called salmonellosis. During the first phase of infection, Salmonella uses its flagella to swim near the surface of the epithelial cells and to target specific site of infection. In order to study the selection criteria that determine which host cells are ...
    Liposome Flotation Assay for Studying Interactions Between Rubella Virus Particles and Lipid Membranes
    用于风疹病毒颗粒和脂膜相互作用研究的脂质体浮选分析法
    作者:Kyoko Saito, Noriyuki Otsuki, Makoto Takeda and Kentaro Hanada日期:08/20/2018,浏览量:6760,Q&A: 0
    [Abstract] Rubella virus (RuV) is an enveloped, positive-sense single-stranded RNA virus that is pathogenic to humans. RuV binds to the target cell via the viral envelope protein E1, but the specific receptor molecules on the target cell are yet to be fully elucidated. Here, we describe a protocol for liposome flotation assay to study direct interactions ...
    Sleeping Beauty Transposon-based System for Rapid Generation of HBV-replicating Stable Cell Lines
    使用基于睡美人转座子的系统快速生成HBV复制稳定细胞系
    作者:Jin-Wei Zheng, Jia-Li Cao and Quan Yuan 日期:07/05/2018,浏览量:6237,Q&A: 0
    [Abstract] The stable HBV-transfected cell lines, which based on stable integration of replication-competent HBV genome into hepatic cells, are widely used in basic research and antiviral drug evaluation against HBV. However, previous reported strategies to generate HBV-replicating cell lines, which primarily rely on random integration of exogenous DNA by ...
    In-vitro and in-planta Botrytis cinerea Inoculation Assays for Tomato
    番茄离体及活体灰葡萄孢菌接种试验
    作者:Jiajie Lian, Hongyu Han, Jiuhai Zhao and Chuanyou Li日期:04/20/2018,浏览量:10031,Q&A: 0
    [Abstract] Botrytis cinerea (B. cinerea) attacks many crops of economic importance, represents one of the most extensively studied necrotrophic pathogens. Inoculation of B. cinerea and phenotypic analysis of plant resistance are key procedures to investigate the mechanism of plant immunity. Here we describe a protocol for B. ...
    Adhesion of Enteroaggregative E. coli Strains to HEK293 Cells
    肠凝聚型大肠埃希杆菌对HEK293细胞的粘附测定
    作者:Jorge Luis Ayala-Lujan and Fernando Ruiz-Perez日期:04/20/2018,浏览量:4747,Q&A: 0
    [Abstract] Enteroaggregative Escherichia coli (EAEC) is a recognized cause of acute diarrhea among both children and adults worldwide. EAEC strains are characterized by the presence of aggregative adherence fimbriae (AAF), which play a key role in pathogenesis by mediating attachment to the intestinal mucosa and by triggering host inflammatory ...