神经科学

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    现刊
    An ex vivo Approach to Assess Mitochondrial ROS by Flow Cytometry in AAV-tagged Astrocytes in Adult Mice
    一种利用流式细胞术进行成年大鼠腺相关病毒标记星型胶质细胞中线粒体ROS水平的体外检测方法
    作者:Carlos Vicente-Gutierrez and Juan P. Bolaños日期:03/20/2020,浏览量:2787,Q&A: 0
    [Abstract] Mitochondrial reactive oxygen species (mROS) are naturally produced signalling molecules extremely relevant for understanding both health- and disease-associated biological processes. The study of mROS in the brain is currently underway to decipher their physiopathological roles and contributions in neurological diseases. Recent advances in this ...
    Analysis of the Mitochondrial Membrane Potential Using the Cationic JC-1 Dye as a Sensitive Fluorescent Probe
    用阳离子JC-1染料作为敏感荧光探针进行线粒体膜电势分析
    作者:Farzane Sivandzade, Aditya Bhalerao and Luca Cucullo日期:01/05/2019,浏览量:15941,Q&A: 0
    [Abstract] In recent years, fluorescent dyes have been frequently used for monitoring mitochondrial membrane potential to evaluate mitochondrial viability and function. However, the reproducibility of the results across laboratories strongly depends upon following well validated and reliable protocols along with the appropriate controls. Herein, we provide a ...
    Image-Based Analysis of Mitochondrial Area and Counting from Adult Mouse Dopaminergic Neurites
    基于图像分析成熟小鼠多巴胺能神经元突起中线粒体的面积和数量
    作者:Nadee Nissanka, Carlos T Moraes and Mlena Pinto日期:08/20/2018,浏览量:6436,Q&A: 0
    [Abstract] Mitochondria form dynamic cytoplasmic networks which undergo morphological changes in order to adapt to cellular stresses and signals. These changes can include alterations in size and number within a given cell. Analysis of the whole network can be a useful metric to assess overall mitochondrial health, particularly in neurons, which are highly ...
    Non-radioactive in vitro PINK1 Kinase Assays Using Ubiquitin or Parkin as Substrate
    采用泛素蛋白或酵素作为底物的非放射性体外PINK1激酶实验
    作者:Fabienne C. Fiesel, Roman Hudec and Wolfdieter Springer日期:10/05/2016,浏览量:6591,Q&A: 0
    [Abstract] This protocol describes the in vitro phosphorylation of ubiquitin and Parkin by the kinase PINK1 using recombinant proteins. Both substrates, ubiquitin and Parkin, are phosphorylated at the conserved serine 65 residue (pS65-ubiquitin and pS65-Parkin). The protocol also includes the use of monomeric and K48- and K63-linked poly-ubiquitin ...
    Isolating Brain Mitochondria by Differential Centrifugation
    差速离心法分离脑线粒体
    作者:Ignacio Amigo, Javier Traba and Carlos B. Rueda日期:05/20/2016,浏览量:14001,Q&A: 3
    [Abstract] In addition to methods aimed at the study of mitochondrial function in-situ, a full understanding of mitochondrial function requires their purification from cells or tissues under specific physiological or pathological conditions. This protocol illustrates a sequential procedure to obtain functional mitochondria with high yield from mice ...
    Mitochondrial Isolation and Purification from Mouse Spinal Cord
    小鼠脊髓的线粒体分离和纯化
    作者:Philippe A. Parone, Sandrine Da Cruz and Don W. Cleveland日期:11/05/2013,浏览量:10118,Q&A: 0
    [Abstract] Mitochondria are eukaryotic organelles that play a crucial role in several cellular processes, including energy production, β-oxidation of fatty acids and regulation of calcium homeostasis. In the last 20 years there has been a hightened interest in the study of mitochondria following the discoveries that mitochondria are central to the process of ...