神经科学

分类

    现刊
    Optimized Immunostaining of Embryonic and Early Postnatal Mouse Brain Sections
    胚胎和产后早期小鼠脑切片的优化免疫染色
    作者:Kawssar Harb, Michele Bertacchi and Michèle Studer日期:01/05/2021,浏览量:1551,Q&A: 0
    [Abstract]

    The mammalian neocortex, the outer layer of the cerebrum and most recently evolved brain region, is characterized by its unique areal and laminar organization. Distinct cortical layers and areas can be identified by the protein expression of graded transcription factors and molecular determinants that define the identity of different projection

    ...
    Staining and Quantitative Analysis of Myelinating Oligodendrocytes in the Mouse Grey Matter
    小鼠灰质髓鞘少突胶质细胞的染色及定量分析
    作者:Matthew Swire and Charles ffrench-Constant日期:10/20/2020,浏览量:1353,Q&A: 0
    [Abstract] Oligodendrocytes generate distinct patterns of myelination throughout the CNS. Variations in myelination along axons may enable neurons to fine-tune conduction velocities and alter signal synchronisation. Here we outline a staining protocol permitting the assessment of the number and length of myelin sheaths formed by oligodendrocyte in the mouse ...
    Dissection and Staining of Mouse Brain Ventricular Wall for the Analysis of Ependymal Cell Cilia Organization
    小鼠脑室管膜细胞纤毛组织分析:脑室的解剖和染色
    作者:Paul Labedan, Cédric Matthews, Laurent Kodjabachian, Harold Cremer, Fadel Tissir and Camille Boutin日期:03/20/2016,浏览量:11571,Q&A: 0
    [Abstract] In the developing and mature central nervous system (CNS) the ventricular lumen is lined by the neuroepithelium and ependymal, respectively. These ventricular epithelia perform important functions related to the development, morphogenesis and physiology of the brain. In the mature CNS, ependyma constitutes a barrier between brain parenchyma and ...
    Combined in situ Hybridization/Immunohistochemistry (ISH/IH) on Free-floating Vibratome Tissue Sections
    自由浮动振动切片机中组织切片的组合原位杂交/免疫组织化学法(ISH/IH)
    作者:Manuel E. Lopez日期:09/20/2014,浏览量:12298,Q&A: 0
    [Abstract] In situ hybridization and immunostaining are common techniques for localizing gene expression, the mRNA and protein respectively, within tissues. Both techniques can be applied to tissue sections to achieve similar goals, but in some cases, it is necessary to use them together. For example, complement C1q is a secreted protein complex ...
    Mouse Cochlear Whole Mount Immunofluorescence
    小鼠耳蜗整装包埋免疫荧光法
    作者:Omar Akil and Lawrence R. Lustig日期:03/05/2013,浏览量:27467,Q&A: 2
    [Abstract] This protocol comprises the entire process of immunofluorescence staining on mouse cochlea whole mount, starting from tissue preparation to the mounting of the tissue. This technique provides “three-dimensional” views of the stained components in order to determine the localization of a protein of interest in the tissue in its natural state and ...
    Immunofluorescence Staining on Mouse Embryonic Brain Sections
    小鼠胚胎脑切片的免疫荧光染色
    作者:Xuecai Ge日期:06/05/2012,浏览量:21266,Q&A: 0
    [Abstract] This protocol comprises the entire process of immunofluorescence staining on mouse embryonic brains, starting from tissue preparation to mounting of the tissue sections.